Partial characterization of sulfohydrolase from Gracilaria dura and evaluation of its potential application in improvement of the agar quality

Shukla, MK; Kumar, M; Prasad, K; Reddy, CRK; Jha, B

Partial characterization of sulfohydrolase from Gracilaria dura and evaluation of its potential application in improvement of the agar quality

Shukla, MK Kumar, M

Prasad, K Reddy, CRK Jha, B

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Publication Type:: Journal Article
Citation:: Carbohydrate Polymers, 2011, 85 (1), pp. 157 - 163
Issue Date:: 2011-04-22

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Field	Value	Language
dc.contributor.author	Shukla, MK	en_US
dc.contributor.author	Kumar, M https://orcid.org/0000-0002-5247-3059	en_US
dc.contributor.author	Prasad, K	en_US
dc.contributor.author	Reddy, CRK	en_US
dc.contributor.author	Jha, B	en_US
dc.date.issued	2011-04-22	en_US
dc.identifier.citation	Carbohydrate Polymers, 2011, 85 (1), pp. 157 - 163	en_US
dc.identifier.issn	0144-8617	en_US
dc.identifier.uri	http://hdl.handle.net/10453/110162
dc.description.abstract	Sulfohydrolase extracted from Gracilaria dura was purified to homogeneity and investigated for improving the quality of commercial agar. The purified enzyme (50 kDa) showed optimum activity at pH 8.0 and temperature 35 °C. The agar treated with ∼50 U of purified enzyme exhibited 1.66-fold increase in 3,6-AG content with 60% sulfate removal and also resulted an increase of ≥2-fold in viscosity and gel strength with a recovery of 90% agar. Further, the gelling and melting temperatures were markedly decreased to 31 °C and 82 °C respectively over the control values of 39 °C and 90 °C. The scanning electron microscopy revealed higher cross-linking and rigidity in the treated agar while FT-IR spectral analysis confirmed the increased 3,6-AG content with decreased sulfate. Therefore, the possibility for cloning of sulfohydrolase encoding gene(s) for its commercial production and exploitation in desulfation of agar could be an eco-friendly and alternative method to alkali treatment. © 2011 Elsevier Ltd. All rights reserved.	en_US
dc.relation.ispartof	Carbohydrate Polymers	en_US
dc.relation.isbasedon	10.1016/j.carbpol.2011.02.009	en_US
dc.subject.classification	Polymers	en_US
dc.title	Partial characterization of sulfohydrolase from Gracilaria dura and evaluation of its potential application in improvement of the agar quality	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	85	en_US
utslib.for	0908 Food Sciences	en_US
utslib.for	0303 Macromolecular and Materials Chemistry	en_US
utslib.for	0305 Organic Chemistry	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	85	en_US

Abstract:

Sulfohydrolase extracted from Gracilaria dura was purified to homogeneity and investigated for improving the quality of commercial agar. The purified enzyme (50 kDa) showed optimum activity at pH 8.0 and temperature 35 °C. The agar treated with ∼50 U of purified enzyme exhibited 1.66-fold increase in 3,6-AG content with 60% sulfate removal and also resulted an increase of ≥2-fold in viscosity and gel strength with a recovery of 90% agar. Further, the gelling and melting temperatures were markedly decreased to 31 °C and 82 °C respectively over the control values of 39 °C and 90 °C. The scanning electron microscopy revealed higher cross-linking and rigidity in the treated agar while FT-IR spectral analysis confirmed the increased 3,6-AG content with decreased sulfate. Therefore, the possibility for cloning of sulfohydrolase encoding gene(s) for its commercial production and exploitation in desulfation of agar could be an eco-friendly and alternative method to alkali treatment. © 2011 Elsevier Ltd. All rights reserved.

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http://hdl.handle.net/10453/110162