Dynamic modelling of cell death during biofilm development

Fagerlind, MG; Webb, JS; Barraud, N; McDougald, D; Jansson, A; Nilsson, P; Harlén, M; Kjelleberg, S; Rice, SA

Dynamic modelling of cell death during biofilm development

Fagerlind, MG Webb, JS Barraud, N McDougald, D

Jansson, A Nilsson, P Harlén, M Kjelleberg, S Rice, SA

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Publication Type:: Journal Article
Citation:: Journal of Theoretical Biology, 2012, 295 pp. 23 - 36
Issue Date:: 2012-02-21

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Field	Value	Language
dc.contributor.author	Fagerlind, MG	en_US
dc.contributor.author	Webb, JS	en_US
dc.contributor.author	Barraud, N	en_US
dc.contributor.author	McDougald, D https://orcid.org/0000-0001-5827-8441	en_US
dc.contributor.author	Jansson, A	en_US
dc.contributor.author	Nilsson, P	en_US
dc.contributor.author	Harlén, M	en_US
dc.contributor.author	Kjelleberg, S	en_US
dc.contributor.author	Rice, SA https://orcid.org/0000-0002-9486-2343	en_US
dc.date.available	2011-10-04	en_US
dc.date.issued	2012-02-21	en_US
dc.identifier.citation	Journal of Theoretical Biology, 2012, 295 pp. 23 - 36	en_US
dc.identifier.issn	0022-5193	en_US
dc.identifier.uri	http://hdl.handle.net/10453/114119
dc.description.abstract	Biofilms are currently recognised as the predominant bacterial life-style and it has been suggested that biofilm development is influenced by a number of different processes such as adhesion, detachment, mass transport, quorum sensing, cell death and active dispersal. One of the least understood processes and its effects on biofilm development is cell death. However, experimental studies suggest that bacterial death is an important process during biofilm development and many studies show a relationship between cell death and dispersal in microbial biofilms. We present a model of the process of cell death during biofilm development, with a particular focus on the spatial localisation of cell death or cell damage. Three rules governing cell death or cell damage were evaluated which compared the effects of starvation, damage accumulation, and viability during biofilm development and were also used to design laboratory based experiments to test the model. Results from model simulations show that actively growing biofilms develop steep nutrient gradients within the interior of the biofilm that affect neighbouring microcolonies resulting in cell death and detachment. Two of the rules indicated that high substrate concentrations lead to accelerated cell death, in contrast to the third rule, based on the accumulation of damage, which predicted earlier cell death for biofilms grown with low substrate concentrations. Comparison of the modelling results with experimental results suggests that cell death is favoured under low nutrient conditions and that the accumulation of damage may be the main cause of cell death during biofilm development. © 2011 Elsevier Ltd.	en_US
dc.relation.ispartof	Journal of Theoretical Biology	en_US
dc.relation.isbasedon	10.1016/j.jtbi.2011.10.007	en_US
dc.subject.classification	Evolutionary Biology	en_US
dc.subject.mesh	Bacteria	en_US
dc.subject.mesh	Biofilms	en_US
dc.subject.mesh	Microscopy, Fluorescence	en_US
dc.subject.mesh	Bacterial Adhesion	en_US
dc.subject.mesh	Bacteriolysis	en_US
dc.subject.mesh	Algorithms	en_US
dc.subject.mesh	Models, Biological	en_US
dc.subject.mesh	Time Factors	en_US
dc.title	Dynamic modelling of cell death during biofilm development	en_US
dc.type	Journal Article
utslib.citation.volume	295	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	01 Mathematical Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	08 Information and Computing Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	295	en_US

Abstract:

Biofilms are currently recognised as the predominant bacterial life-style and it has been suggested that biofilm development is influenced by a number of different processes such as adhesion, detachment, mass transport, quorum sensing, cell death and active dispersal. One of the least understood processes and its effects on biofilm development is cell death. However, experimental studies suggest that bacterial death is an important process during biofilm development and many studies show a relationship between cell death and dispersal in microbial biofilms. We present a model of the process of cell death during biofilm development, with a particular focus on the spatial localisation of cell death or cell damage. Three rules governing cell death or cell damage were evaluated which compared the effects of starvation, damage accumulation, and viability during biofilm development and were also used to design laboratory based experiments to test the model. Results from model simulations show that actively growing biofilms develop steep nutrient gradients within the interior of the biofilm that affect neighbouring microcolonies resulting in cell death and detachment. Two of the rules indicated that high substrate concentrations lead to accelerated cell death, in contrast to the third rule, based on the accumulation of damage, which predicted earlier cell death for biofilms grown with low substrate concentrations. Comparison of the modelling results with experimental results suggests that cell death is favoured under low nutrient conditions and that the accumulation of damage may be the main cause of cell death during biofilm development. © 2011 Elsevier Ltd.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/114119