Selenium and spermine alleviate cadmium induced toxicity in the red seaweed Gracilaria dura by regulating antioxidants and DNA methylation

Kumar, M; Bijo, AJ; Baghel, RS; Reddy, CRK; Jha, B

Selenium and spermine alleviate cadmium induced toxicity in the red seaweed Gracilaria dura by regulating antioxidants and DNA methylation

Kumar, M

Bijo, AJ Baghel, RS Reddy, CRK Jha, B

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Publication Type:: Journal Article
Citation:: Plant Physiology and Biochemistry, 2012, 51 pp. 129 - 138
Issue Date:: 2012-02-01

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Field	Value	Language
dc.contributor.author	Kumar, M https://orcid.org/0000-0002-5247-3059	en_US
dc.contributor.author	Bijo, AJ	en_US
dc.contributor.author	Baghel, RS	en_US
dc.contributor.author	Reddy, CRK	en_US
dc.contributor.author	Jha, B	en_US
dc.date.available	2011-10-24	en_US
dc.date.issued	2012-02-01	en_US
dc.identifier.citation	Plant Physiology and Biochemistry, 2012, 51 pp. 129 - 138	en_US
dc.identifier.issn	0981-9428	en_US
dc.identifier.uri	http://hdl.handle.net/10453/114245
dc.description.abstract	The protective role of exogenously supplied selenium (Se) and polyamines (PAs) such as putrescine (Put) and spermine (Spm) in detoxifying the cadmium (Cd) induced toxicity was studied in the marine red alga Gracilaria dura in laboratory conditions. The Cd exposure (0.4 mM) impede the growth of alga while triggering the reactive oxygen species (ROS viz. O 2•- and H 2O 2) generation, inhibition of antioxidant system, and enhancing the lipoxygenase (LOX) activity, malondialdehyde (MDA) level and demethylation of DNA. Additions of Se (50 μM) and/or Spm (1 mM) to the culture medium in contrast to Put, efficiently ameliorated the Cd toxicity by decreasing the accumulation of ROS and MDA contents, while restoring or enhancing the level of enzymatic and nonenzymatic antioxidants and their redox ratio, phycobiliproteins and phytochelatins, over the controls. The isoforms of antioxidant enzymes namely superoxide dismutase (Mn-SOD, ∼ 150 kDa; Fe-SOD ∼120 kDa), glutathione peroxidase (GSH-Px, ∼120 and 140 kDa), glutathione reductase (GR, ∼110 kDa) regulated differentially to Se and/or Spm supplementation. Furthermore, it has also resulted in enhanced levels of endogenous PAs (specially free and bound insoluble Put and Spm) and n-6 PUFAs (C20-3, n-6 and C20-4, n-6). This is for the first time wherein Se and Spm were found to regulate the stabilization of DNA methylation by reducing the events of cytosine demethylation in a mechanism to alleviate the Cd stress in marine alga. The present findings reveal that both Se and Spm play a crucial role in controlling the Cd induced oxidative stress in G. dura. © 2011 Elsevier Masson SAS.	en_US
dc.relation.ispartof	Plant Physiology and Biochemistry	en_US
dc.relation.isbasedon	10.1016/j.plaphy.2011.10.016	en_US
dc.subject.classification	Plant Biology & Botany	en_US
dc.subject.mesh	Gracilaria	en_US
dc.subject.mesh	Hydrogen Peroxide	en_US
dc.subject.mesh	Selenium	en_US
dc.subject.mesh	Cadmium	en_US
dc.subject.mesh	Reactive Oxygen Species	en_US
dc.subject.mesh	Malondialdehyde	en_US
dc.subject.mesh	Spermine	en_US
dc.subject.mesh	Ascorbic Acid	en_US
dc.subject.mesh	Glutathione Reductase	en_US
dc.subject.mesh	Lipoxygenase	en_US
dc.subject.mesh	Glutathione Peroxidase	en_US
dc.subject.mesh	Superoxide Dismutase	en_US
dc.subject.mesh	Antioxidants	en_US
dc.subject.mesh	Culture Media	en_US
dc.subject.mesh	DNA Methylation	en_US
dc.subject.mesh	Enzyme Activation	en_US
dc.subject.mesh	Oxidation-Reduction	en_US
dc.subject.mesh	Lipid Peroxidation	en_US
dc.subject.mesh	Oxidative Stress	en_US
dc.subject.mesh	Solubility	en_US
dc.subject.mesh	Phycobiliproteins	en_US
dc.subject.mesh	Phytochelatins	en_US
dc.subject.mesh	Enzyme Assays	en_US
dc.title	Selenium and spermine alleviate cadmium induced toxicity in the red seaweed Gracilaria dura by regulating antioxidants and DNA methylation	en_US
dc.type	Journal Article
utslib.citation.volume	51	en_US
utslib.for	0503 Soil Sciences	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	0501 Ecological Applications	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	51	en_US

Abstract:

The protective role of exogenously supplied selenium (Se) and polyamines (PAs) such as putrescine (Put) and spermine (Spm) in detoxifying the cadmium (Cd) induced toxicity was studied in the marine red alga Gracilaria dura in laboratory conditions. The Cd exposure (0.4 mM) impede the growth of alga while triggering the reactive oxygen species (ROS viz. O 2•- and H 2O 2) generation, inhibition of antioxidant system, and enhancing the lipoxygenase (LOX) activity, malondialdehyde (MDA) level and demethylation of DNA. Additions of Se (50 μM) and/or Spm (1 mM) to the culture medium in contrast to Put, efficiently ameliorated the Cd toxicity by decreasing the accumulation of ROS and MDA contents, while restoring or enhancing the level of enzymatic and nonenzymatic antioxidants and their redox ratio, phycobiliproteins and phytochelatins, over the controls. The isoforms of antioxidant enzymes namely superoxide dismutase (Mn-SOD, ∼ 150 kDa; Fe-SOD ∼120 kDa), glutathione peroxidase (GSH-Px, ∼120 and 140 kDa), glutathione reductase (GR, ∼110 kDa) regulated differentially to Se and/or Spm supplementation. Furthermore, it has also resulted in enhanced levels of endogenous PAs (specially free and bound insoluble Put and Spm) and n-6 PUFAs (C20-3, n-6 and C20-4, n-6). This is for the first time wherein Se and Spm were found to regulate the stabilization of DNA methylation by reducing the events of cytosine demethylation in a mechanism to alleviate the Cd stress in marine alga. The present findings reveal that both Se and Spm play a crucial role in controlling the Cd induced oxidative stress in G. dura. © 2011 Elsevier Masson SAS.

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http://hdl.handle.net/10453/114245