Laser oblique scanning optical microscopy (LOSOM) for phase relief imaging

Ding, Y; Xie, H; Peng, T; Lu, Y; Jin, D; Teng, J; Ren, Q; Xi, P

Laser oblique scanning optical microscopy (LOSOM) for phase relief imaging

Ding, Y Xie, H Peng, T Lu, Y Jin, D

Teng, J Ren, Q Xi, P

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Publication Type:: Journal Article
Citation:: Optics Express, 2012, 20 (13), pp. 14100 - 14108
Issue Date:: 2012-06-18

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Field	Value	Language
dc.contributor.author	Ding, Y	en_US
dc.contributor.author	Xie, H	en_US
dc.contributor.author	Peng, T	en_US
dc.contributor.author	Lu, Y	en_US
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666	en_US
dc.contributor.author	Teng, J	en_US
dc.contributor.author	Ren, Q	en_US
dc.contributor.author	Xi, P	en_US
dc.date.issued	2012-06-18	en_US
dc.identifier.citation	Optics Express, 2012, 20 (13), pp. 14100 - 14108	en_US
dc.identifier.uri	http://hdl.handle.net/10453/114471
dc.description.abstract	The visualization of optical phase can provide abundant information when imaging transparent specimen. We present a novel phase sensitive imaging design capable of obtaining phase contours of transparent biological cells through laser oblique scanning optical microscope (LOSOM). LOSOM is based on the introduction of a fluorescent medium behind the specimen to generate a differential phase-sensitive image, thus, the complicated phase retardation alignment procedure associated with differential interference contrast (DIC) microscopy can be eliminated. Moreover, multi-modality fluorescence and phase relief imaging can be attained in a single system with fluorescently labeled specimens. © 2012 Optical Society of America.	en_US
dc.relation.ispartof	Optics Express	en_US
dc.relation.isbasedon	10.1364/OE.20.014100	en_US
dc.rights	© 2012 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.	en_US
dc.subject.classification	Optics	en_US
dc.subject.mesh	Microscopy, Confocal	en_US
dc.subject.mesh	Microscopy, Phase-Contrast	en_US
dc.subject.mesh	Sensitivity and Specificity	en_US
dc.subject.mesh	Reproducibility of Results	en_US
dc.subject.mesh	Equipment Design	en_US
dc.subject.mesh	Equipment Failure Analysis	en_US
dc.title	Laser oblique scanning optical microscopy (LOSOM) for phase relief imaging	en_US
dc.type	Journal Article
utslib.citation.volume	13	en_US
utslib.citation.volume	20	en_US
utslib.for	0205 Optical Physics	en_US
utslib.for	0906 Electrical and Electronic Engineering	en_US
utslib.for	1005 Communications Technologies	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access
pubs.issue	13	en_US
pubs.publication-status	Published	en_US
pubs.volume	20	en_US

Abstract:

The visualization of optical phase can provide abundant information when imaging transparent specimen. We present a novel phase sensitive imaging design capable of obtaining phase contours of transparent biological cells through laser oblique scanning optical microscope (LOSOM). LOSOM is based on the introduction of a fluorescent medium behind the specimen to generate a differential phase-sensitive image, thus, the complicated phase retardation alignment procedure associated with differential interference contrast (DIC) microscopy can be eliminated. Moreover, multi-modality fluorescence and phase relief imaging can be attained in a single system with fluorescently labeled specimens. © 2012 Optical Society of America.

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http://hdl.handle.net/10453/114471