Antimicrobial activity of immobilized lactoferrin and lactoferricin

Chen, R; Cole, N; Dutta, D; Kumar, N; Willcox, MDP

Antimicrobial activity of immobilized lactoferrin and lactoferricin

Chen, R Cole, N

Dutta, D Kumar, N Willcox, MDP

Permalink

Publication Type:: Journal Article
Citation:: Journal of Biomedical Materials Research - Part B Applied Biomaterials, 2017, 105 (8), pp. 2612 - 2617
Issue Date:: 2017-11-01

Closed Access

	Filename	Description	Size
	Chen_et_al-2016-Journal_of_Biomedical_Materials_Research_Part_B-_Applied_Biomaterials.pdf	Published Version	301.92 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Chen, R	en_US
dc.contributor.author	Cole, N https://orcid.org/0000-0002-9866-4694	en_US
dc.contributor.author	Dutta, D	en_US
dc.contributor.author	Kumar, N	en_US
dc.contributor.author	Willcox, MDP	en_US
dc.date.available	2016-10-08	en_US
dc.date.issued	2017-11-01	en_US
dc.identifier.citation	Journal of Biomedical Materials Research - Part B Applied Biomaterials, 2017, 105 (8), pp. 2612 - 2617	en_US
dc.identifier.issn	1552-4973	en_US
dc.identifier.uri	http://hdl.handle.net/10453/114728
dc.description.abstract	© 2016 Wiley Periodicals, Inc. Lactoferrin and lactoferricin were immobilized on glass surfaces via two linkers, 4-azidobenzoic acid (ABA) or 4-fluoro-3-nitrophenyl azide (FNA). The resulting surfaces were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle measurements. The antimicrobial activity of the surfaces was determined using Pseudomonas aeruginosa and Staphylococcus aureus strains by fluorescence microscopy. Lactoferrin and lactoferricin immobilization was confirmed by XPS showing significant increases (p < 0.05) in nitrogen on the glass surface. The immobilization of both proteins slightly increased the overall hydrophobicity of the glass. Both lactoferrin and lactoferricin immobilized on glass significantly (p < 0.05) reduced the numbers of viable bacterial cells adherent to the glass. For P. aeruginosa, the immobilized proteins consistently increased the percentage of dead cells compared to the total cells adherent to the glass surfaces (p < 0.03). Lactoferrin and lactoferricin were successfully immobilized on glass surfaces and showed promising antimicrobial activity against pathogenic bacteria. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2612–2617, 2017.	en_US
dc.relation.ispartof	Journal of Biomedical Materials Research - Part B Applied Biomaterials	en_US
dc.relation.isbasedon	10.1002/jbm.b.33804	en_US
dc.subject.classification	Biomedical Engineering	en_US
dc.subject.mesh	Pseudomonas aeruginosa	en_US
dc.subject.mesh	Staphylococcus aureus	en_US
dc.subject.mesh	Glass	en_US
dc.subject.mesh	Lactoferrin	en_US
dc.subject.mesh	Anti-Bacterial Agents	en_US
dc.subject.mesh	Immobilized Proteins	en_US
dc.title	Antimicrobial activity of immobilized lactoferrin and lactoferricin	en_US
dc.type	Journal Article
utslib.citation.volume	8	en_US
utslib.citation.volume	105	en_US
utslib.for	0903 Biomedical Engineering	en_US
utslib.for	0912 Materials Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	closed_access
pubs.issue	8	en_US
pubs.publication-status	Published	en_US
pubs.volume	105	en_US

Abstract:

© 2016 Wiley Periodicals, Inc. Lactoferrin and lactoferricin were immobilized on glass surfaces via two linkers, 4-azidobenzoic acid (ABA) or 4-fluoro-3-nitrophenyl azide (FNA). The resulting surfaces were characterized by X-ray photoelectron spectroscopy (XPS) and contact angle measurements. The antimicrobial activity of the surfaces was determined using Pseudomonas aeruginosa and Staphylococcus aureus strains by fluorescence microscopy. Lactoferrin and lactoferricin immobilization was confirmed by XPS showing significant increases (p < 0.05) in nitrogen on the glass surface. The immobilization of both proteins slightly increased the overall hydrophobicity of the glass. Both lactoferrin and lactoferricin immobilized on glass significantly (p < 0.05) reduced the numbers of viable bacterial cells adherent to the glass. For P. aeruginosa, the immobilized proteins consistently increased the percentage of dead cells compared to the total cells adherent to the glass surfaces (p < 0.03). Lactoferrin and lactoferricin were successfully immobilized on glass surfaces and showed promising antimicrobial activity against pathogenic bacteria. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2612–2617, 2017.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/114728