Proteomic and genomic analyses suggest the association of apolipoprotein C1 with abdominal aortic aneurysm

Moxon, JV; Liu, D; Moran, CS; Crossman, DJ; Krishna, SM; Yonglitthipagon, P; Emeto, TI; Morris, DR; Padula, MP; Mulvenna, JP; Rush, CM; Golledge, J

Proteomic and genomic analyses suggest the association of apolipoprotein C1 with abdominal aortic aneurysm

Moxon, JV Liu, D Moran, CS Crossman, DJ Krishna, SM Yonglitthipagon, P Emeto, TI Morris, DR Padula, MP

Mulvenna, JP Rush, CM Golledge, J

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Publication Type:: Journal Article
Citation:: Proteomics - Clinical Applications, 2014, 8 (9-10), pp. 762 - 772
Issue Date:: 2014-10-01

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Field	Value	Language
dc.contributor.author	Moxon, JV	en_US
dc.contributor.author	Liu, D	en_US
dc.contributor.author	Moran, CS	en_US
dc.contributor.author	Crossman, DJ	en_US
dc.contributor.author	Krishna, SM	en_US
dc.contributor.author	Yonglitthipagon, P	en_US
dc.contributor.author	Emeto, TI	en_US
dc.contributor.author	Morris, DR	en_US
dc.contributor.author	Padula, MP https://orcid.org/0000-0002-8283-0643	en_US
dc.contributor.author	Mulvenna, JP	en_US
dc.contributor.author	Rush, CM	en_US
dc.contributor.author	Golledge, J https://orcid.org/0000-0002-5779-8848	en_US
dc.date.available	2014-01-27	en_US
dc.date.issued	2014-10-01	en_US
dc.identifier.citation	Proteomics - Clinical Applications, 2014, 8 (9-10), pp. 762 - 772	en_US
dc.identifier.issn	1862-8346	en_US
dc.identifier.uri	http://hdl.handle.net/10453/115561
dc.description.abstract	© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Purpose: Abdominal aortic aneurysm (AAA) is an important cause of mortality in the elderly. Mouse models are widely used to investigate AAA pathogenesis but their suitability for biomarker discovery is unexplored. Experimental design: We conducted a three-phase study. Phase 1: Aortas from angiotensin-II-infused apolipoprotein E deficient (ApoE-/-) mice with and without AAA were assessed via iTRAQ and analyzed in silico to identify potential circulating markers. Microarray data from ApoE-/- mice and human patients were analyzed in parallel. Phase 2: Putative markers were compared between datasets to shortlist common candidates. Phase 3: The relationship of two shortlisted markers and AAA presence was assessed. Results: iTRAQ identified eight proteins with biomarker potential. Microarray data identified 72 and 96 potential biomarkers from ApoE-/- mice and human patients, respectively. All three datasets suggested apolipoprotein C1 (ApoC1) as a marker for AAA; microarray data identified matrix metalloproteinase 9 (MMP9) as a second potential marker. Plasma ApoC1 and MMP9 concentrations positively correlated with AAA diameter in ApoE-/- mice. Conclusions and clinical relevance: ApoC1 may be a novel biomarker for AAA.	en_US
dc.relation.ispartof	Proteomics - Clinical Applications	en_US
dc.relation.isbasedon	10.1002/prca.201300119	en_US
dc.subject.classification	Biochemistry & Molecular Biology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice, Knockout	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Aortic Aneurysm, Abdominal	en_US
dc.subject.mesh	Apolipoproteins E	en_US
dc.subject.mesh	Biological Markers	en_US
dc.subject.mesh	Microscopy, Confocal	en_US
dc.subject.mesh	Chromatography, Liquid	en_US
dc.subject.mesh	Gene Expression Profiling	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Apolipoprotein C-I	en_US
dc.subject.mesh	Tandem Mass Spectrometry	en_US
dc.subject.mesh	Biomarkers	en_US
dc.title	Proteomic and genomic analyses suggest the association of apolipoprotein C1 with abdominal aortic aneurysm	en_US
dc.type	Journal Article
utslib.citation.volume	9-10	en_US
utslib.citation.volume	8	en_US
utslib.for	1101 Medical Biochemistry and Metabolomics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	9-10	en_US
pubs.publication-status	Published	en_US
pubs.volume	8	en_US

Abstract:

© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Purpose: Abdominal aortic aneurysm (AAA) is an important cause of mortality in the elderly. Mouse models are widely used to investigate AAA pathogenesis but their suitability for biomarker discovery is unexplored. Experimental design: We conducted a three-phase study. Phase 1: Aortas from angiotensin-II-infused apolipoprotein E deficient (ApoE-/-) mice with and without AAA were assessed via iTRAQ and analyzed in silico to identify potential circulating markers. Microarray data from ApoE-/- mice and human patients were analyzed in parallel. Phase 2: Putative markers were compared between datasets to shortlist common candidates. Phase 3: The relationship of two shortlisted markers and AAA presence was assessed. Results: iTRAQ identified eight proteins with biomarker potential. Microarray data identified 72 and 96 potential biomarkers from ApoE-/- mice and human patients, respectively. All three datasets suggested apolipoprotein C1 (ApoC1) as a marker for AAA; microarray data identified matrix metalloproteinase 9 (MMP9) as a second potential marker. Plasma ApoC1 and MMP9 concentrations positively correlated with AAA diameter in ApoE-/- mice. Conclusions and clinical relevance: ApoC1 may be a novel biomarker for AAA.

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http://hdl.handle.net/10453/115561