Heavy metal staining, a comparative assessment of gadolinium chloride and osmium tetroxide for inner ear labyrinthine contrast enhancement using X-ray microtomography

Wong, CC; Curthoys, IS; O'leary, SJ; Jones, AS

Heavy metal staining, a comparative assessment of gadolinium chloride and osmium tetroxide for inner ear labyrinthine contrast enhancement using X-ray microtomography

Wong, CC Curthoys, IS O'leary, SJ Jones, AS

Permalink

Publication Type:: Journal Article
Citation:: Acta Oto-Laryngologica, 2013, 133 (1), pp. 22 - 27
Issue Date:: 2013-01-01

Closed Access

	Filename	Description	Size
	Heavy metal staining a comparative assessment of gadolinium chloride and osmium tetroxide for inner ear labyrinthine contrast enhancement using X ray.pdf	Published Version	471.67 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Wong, CC	en_US
dc.contributor.author	Curthoys, IS	en_US
dc.contributor.author	O'leary, SJ	en_US
dc.contributor.author	Jones, AS https://orcid.org/0000-0002-3522-6371	en_US
dc.date.issued	2013-01-01	en_US
dc.identifier.citation	Acta Oto-Laryngologica, 2013, 133 (1), pp. 22 - 27	en_US
dc.identifier.issn	0001-6489	en_US
dc.identifier.uri	http://hdl.handle.net/10453/117395
dc.description.abstract	Conclusion: The use of both gadolinium chloride (GdCl3) and osmium tetroxide (OsO4) allowed for the visualization of the membranous labyrinth and other intralabyrinthine structures, at different intensities, as compared with the control sample. This initial comparison shows the advantages of GdCl3 in radiological assessments and OsO4 in more detailed anatomical studies and pathways of labyrinthine pathogenesis using X-ray microtomography (microCT). Objective: To assess an improved OsO4 staining protocol and compare the staining affinities against GdCl3. Methods: Guinea pig temporal bones were stained with either GdCl3 (2% w/v) for 7 days or OsO4 (2% w/v) for 3 days, and scanned in a microCT system. The post-scanned datasets were then assessed in a 3D rendering program. Results: The enhanced soft tissue contrast as presented in the temporal bones stained with either GdCl3 or OsO4 allowed for the membranous labyrinth to be visualized throughout the whole specimen. GdCl3-stained specimens presented more defined contours of the bone profile in the radiographs, while OsO4-stained specimens provided more anatomical detail of individual intralabyrinthine structures, hence allowing spatial relationships to be visualized with ease in a 3D rendering context and 2D axial slice images. © 2013 Informa Healthcare.	en_US
dc.relation.ispartof	Acta Oto-Laryngologica	en_US
dc.relation.isbasedon	10.3109/00016489.2012.715751	en_US
dc.subject.classification	Public Health	en_US
dc.subject.classification	Otorhinolaryngology	en_US
dc.subject.mesh	Temporal Bone	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Guinea Pigs	en_US
dc.subject.mesh	Gadolinium	en_US
dc.subject.mesh	Osmium Tetroxide	en_US
dc.subject.mesh	Contrast Media	en_US
dc.subject.mesh	Imaging, Three-Dimensional	en_US
dc.subject.mesh	Staining and Labeling	en_US
dc.subject.mesh	Ear, Inner	en_US
dc.subject.mesh	X-Ray Microtomography	en_US
dc.title	Heavy metal staining, a comparative assessment of gadolinium chloride and osmium tetroxide for inner ear labyrinthine contrast enhancement using X-ray microtomography	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	133	en_US
utslib.for	1101 Medical Biochemistry and Metabolomics	en_US
utslib.for	1103 Clinical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	133	en_US

Abstract:

Conclusion: The use of both gadolinium chloride (GdCl3) and osmium tetroxide (OsO4) allowed for the visualization of the membranous labyrinth and other intralabyrinthine structures, at different intensities, as compared with the control sample. This initial comparison shows the advantages of GdCl3 in radiological assessments and OsO4 in more detailed anatomical studies and pathways of labyrinthine pathogenesis using X-ray microtomography (microCT). Objective: To assess an improved OsO4 staining protocol and compare the staining affinities against GdCl3. Methods: Guinea pig temporal bones were stained with either GdCl3 (2% w/v) for 7 days or OsO4 (2% w/v) for 3 days, and scanned in a microCT system. The post-scanned datasets were then assessed in a 3D rendering program. Results: The enhanced soft tissue contrast as presented in the temporal bones stained with either GdCl3 or OsO4 allowed for the membranous labyrinth to be visualized throughout the whole specimen. GdCl3-stained specimens presented more defined contours of the bone profile in the radiographs, while OsO4-stained specimens provided more anatomical detail of individual intralabyrinthine structures, hence allowing spatial relationships to be visualized with ease in a 3D rendering context and 2D axial slice images. © 2013 Informa Healthcare.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/117395