Analytical description of high-aperture STED resolution with 0-2π vortex phase modulation

Xie, H; Liu, Y; Jin, D; Santangelo, PJ; Xi, P

Analytical description of high-aperture STED resolution with 0-2π vortex phase modulation

Xie, H Liu, Y Jin, D

Santangelo, PJ Xi, P

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Publication Type:: Journal Article
Citation:: Journal of the Optical Society of America A: Optics and Image Science, and Vision, 2013, 30 (8), pp. 1640 - 1645
Issue Date:: 2013-01-01

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Field	Value	Language
dc.contributor.author	Xie, H	en_US
dc.contributor.author	Liu, Y	en_US
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666	en_US
dc.contributor.author	Santangelo, PJ	en_US
dc.contributor.author	Xi, P	en_US
dc.date.issued	2013-01-01	en_US
dc.identifier.citation	Journal of the Optical Society of America A: Optics and Image Science, and Vision, 2013, 30 (8), pp. 1640 - 1645	en_US
dc.identifier.issn	1084-7529	en_US
dc.identifier.uri	http://hdl.handle.net/10453/118004
dc.description.abstract	Stimulated emission depletion (STED) can achieve optical superresolution, with the optical diffraction limit broken by the suppression on the periphery of the fluorescent focal spot. Previously, it is generally experimentally accepted that there exists an inverse square root relationship with the STED power and the resolution, but with arbitrary coefficients in expression. In this paper, we have removed the arbitrary coefficients by exploring the relationship between the STED power and the achievable resolution from vector optical theory for the widely used 0-2π vortex phase modulation. Electromagnetic fields of the focal region of a high numerical aperture objective are calculated and approximated into polynomials of radius in the focal plane, and analytical expression of resolution as a function of the STED intensity has been derived. As a result, the resolution can be estimated directly from the measurement of the saturation power of the dye and the STED power applied in the region of high STED power. © 2013 Optical Society of America.	en_US
dc.relation.ispartof	Journal of the Optical Society of America A: Optics and Image Science, and Vision	en_US
dc.relation.isbasedon	10.1364/JOSAA.30.001640	en_US
dc.rights	© 2013 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved.	en_US
dc.subject.classification	Optics	en_US
dc.subject.mesh	Image Interpretation, Computer-Assisted	en_US
dc.subject.mesh	Microscopy, Fluorescence	en_US
dc.subject.mesh	Image Enhancement	en_US
dc.subject.mesh	Sensitivity and Specificity	en_US
dc.subject.mesh	Reproducibility of Results	en_US
dc.subject.mesh	Algorithms	en_US
dc.title	Analytical description of high-aperture STED resolution with 0-2π vortex phase modulation	en_US
dc.type	Journal Article
utslib.citation.volume	8	en_US
utslib.citation.volume	30	en_US
utslib.for	0205 Optical Physics	en_US
utslib.for	0906 Electrical and Electronic Engineering	en_US
utslib.for	1113 Opthalmology and Optometry	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access
pubs.issue	8	en_US
pubs.publication-status	Published	en_US
pubs.volume	30	en_US

Abstract:

Stimulated emission depletion (STED) can achieve optical superresolution, with the optical diffraction limit broken by the suppression on the periphery of the fluorescent focal spot. Previously, it is generally experimentally accepted that there exists an inverse square root relationship with the STED power and the resolution, but with arbitrary coefficients in expression. In this paper, we have removed the arbitrary coefficients by exploring the relationship between the STED power and the achievable resolution from vector optical theory for the widely used 0-2π vortex phase modulation. Electromagnetic fields of the focal region of a high numerical aperture objective are calculated and approximated into polynomials of radius in the focal plane, and analytical expression of resolution as a function of the STED intensity has been derived. As a result, the resolution can be estimated directly from the measurement of the saturation power of the dye and the STED power applied in the region of high STED power. © 2013 Optical Society of America.

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http://hdl.handle.net/10453/118004