Effect of biomimetic zinc-containing tricalcium phosphate (Zn-TCP) on the growth and osteogenic differentiation of mesenchymal stem cells

Chou, J; Hao, J; Hatoyama, H; Ben-Nissan, B; Milthorpe, B; Otsuka, M

Effect of biomimetic zinc-containing tricalcium phosphate (Zn-TCP) on the growth and osteogenic differentiation of mesenchymal stem cells

Chou, J

Hao, J Hatoyama, H Ben-Nissan, B Milthorpe, B

Otsuka, M

Permalink

Publication Type:: Journal Article
Citation:: Journal of Tissue Engineering and Regenerative Medicine, 2015, 9 (7), pp. 852 - 858
Issue Date:: 2015-07-01

Closed Access

	Filename	Description	Size
	chou2014-1.pdf	Published Version	1.33 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Chou, J https://orcid.org/0000-0002-7472-8016	en_US
dc.contributor.author	Hao, J	en_US
dc.contributor.author	Hatoyama, H	en_US
dc.contributor.author	Ben-Nissan, B	en_US
dc.contributor.author	Milthorpe, B https://orcid.org/0000-0002-0867-9586	en_US
dc.contributor.author	Otsuka, M	en_US
dc.date.available	2014-03-17	en_US
dc.date.issued	2015-07-01	en_US
dc.identifier.citation	Journal of Tissue Engineering and Regenerative Medicine, 2015, 9 (7), pp. 852 - 858	en_US
dc.identifier.issn	1932-6254	en_US
dc.identifier.uri	http://hdl.handle.net/10453/120310
dc.description.abstract	© 2014 John Wiley & Sons, Ltd. Several studies have shown the effectiveness of zinc-tricalcium phosphate (Zn-TCP) for bone tissue engineering. In this study, marine calcareous foraminifera possessing uniform pore size distribution were hydrothermally converted to Zn-TCP. The ability of a scaffold to combine effectively with mesenchymal stem cells (MSCs) is a key tissue-engineering aim. In order to demonstrate the osteogenic ability of MSCs with Zn-TCP, the scaffolds were cultured in an osteogenic induction medium to elicit an osteoblastic response. The physicochemical properties of Zn-TCP were characterized by XRD, FT-IR and ICP-MS. MSCs were aspirated from rat femurs and cultured for 3 days before indirectly placing four samples into each respective well. After culture for 7, 10 and 14 days, osteoblastic differentiation was evaluated using alizarin red S stain, measurement of alkaline phosphatase (ALP) levels, cell numbers and cell viability. XRD and FT-IR patterns both showed the replacement of CO32- with PO43-. Chemical analysis showed zinc incorporation of 5 mol%. Significant increases in cell numbers were observed at 10 and 14 days in the Zn-TCP group, while maintaining high levels of cell viability (> 90%). ALP activity in the Zn-TCP group was statistically higher at 10 days. Alizarin red S staining also showed significantly higher levels of calcium mineralization in Zn-TCP compared with the control groups. This study showed that MSCs in the presence of biomimetically derived Zn-TCP can accelerate their differentiation to osteoblasts and could potentially be useful as a scaffold for bone tissue engineering.	en_US
dc.relation.ispartof	Journal of Tissue Engineering and Regenerative Medicine	en_US
dc.relation.isbasedon	10.1002/term.1901	en_US
dc.subject.classification	Biomedical Engineering	en_US
dc.subject.mesh	Cells, Cultured	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Rats	en_US
dc.subject.mesh	Rats, Wistar	en_US
dc.subject.mesh	Calcium Phosphates	en_US
dc.subject.mesh	Zinc	en_US
dc.subject.mesh	Tissue Engineering	en_US
dc.subject.mesh	Cell Differentiation	en_US
dc.subject.mesh	Osteogenesis	en_US
dc.subject.mesh	Biomimetic Materials	en_US
dc.subject.mesh	Male	en_US
dc.subject.mesh	Mesenchymal Stromal Cells	en_US
dc.subject.mesh	Mesenchymal Stem Cells	en_US
dc.title	Effect of biomimetic zinc-containing tricalcium phosphate (Zn-TCP) on the growth and osteogenic differentiation of mesenchymal stem cells	en_US
dc.type	Journal Article
utslib.citation.volume	7	en_US
utslib.citation.volume	9	en_US
utslib.for	1116 Medical Physiology	en_US
utslib.for	0903 Biomedical Engineering	en_US
utslib.for	1103 Clinical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.issue	7	en_US
pubs.publication-status	Published	en_US
pubs.volume	9	en_US

Abstract:

© 2014 John Wiley & Sons, Ltd. Several studies have shown the effectiveness of zinc-tricalcium phosphate (Zn-TCP) for bone tissue engineering. In this study, marine calcareous foraminifera possessing uniform pore size distribution were hydrothermally converted to Zn-TCP. The ability of a scaffold to combine effectively with mesenchymal stem cells (MSCs) is a key tissue-engineering aim. In order to demonstrate the osteogenic ability of MSCs with Zn-TCP, the scaffolds were cultured in an osteogenic induction medium to elicit an osteoblastic response. The physicochemical properties of Zn-TCP were characterized by XRD, FT-IR and ICP-MS. MSCs were aspirated from rat femurs and cultured for 3 days before indirectly placing four samples into each respective well. After culture for 7, 10 and 14 days, osteoblastic differentiation was evaluated using alizarin red S stain, measurement of alkaline phosphatase (ALP) levels, cell numbers and cell viability. XRD and FT-IR patterns both showed the replacement of CO32- with PO43-. Chemical analysis showed zinc incorporation of 5 mol%. Significant increases in cell numbers were observed at 10 and 14 days in the Zn-TCP group, while maintaining high levels of cell viability (> 90%). ALP activity in the Zn-TCP group was statistically higher at 10 days. Alizarin red S staining also showed significantly higher levels of calcium mineralization in Zn-TCP compared with the control groups. This study showed that MSCs in the presence of biomimetically derived Zn-TCP can accelerate their differentiation to osteoblasts and could potentially be useful as a scaffold for bone tissue engineering.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/120310