Brillouin microscopy, what is it really measuring?

Wu, P-J; Kabakova, I; Ruberti, J; Sherwood, JM; Dunlop, IE; Paterson, C; Török, P; Overby, DR

Brillouin microscopy, what is it really measuring?

Wu, P-J Kabakova, I

Ruberti, J Sherwood, JM Dunlop, IE Paterson, C Török, P Overby, DR

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Field	Value	Language
dc.contributor.author	Wu, P-J	en_US
dc.contributor.author	Kabakova, I https://orcid.org/0000-0002-6831-9478	en_US
dc.contributor.author	Ruberti, J	en_US
dc.contributor.author	Sherwood, JM	en_US
dc.contributor.author	Dunlop, IE	en_US
dc.contributor.author	Paterson, C	en_US
dc.contributor.author	Török, P	en_US
dc.contributor.author	Overby, DR	en_US
dc.identifier.uri	http://hdl.handle.net/10453/122051
dc.description.abstract	Brillouin microscopy measures compressibility, but is being increasingly used to assess stiffness of cells and tissues. Using hydrogels with tunable properties, we demonstrate that Brillouin microscopy is insensitive to stiffness of hydrated materials, but depends strongly on water content, consistent with a theoretical model of biphasic compressibility. Empirical correlations between Brillouin measurements and stiffness arise due to their mutual dependence on water content, but correlations vanish once hydration is controlled.	en_US
dc.subject.classification	Developmental Biology	en_US
dc.title	Brillouin microscopy, what is it really measuring?	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	10 Technology	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	open_access

Abstract:

Brillouin microscopy measures compressibility, but is being increasingly used to assess stiffness of cells and tissues. Using hydrogels with tunable properties, we demonstrate that Brillouin microscopy is insensitive to stiffness of hydrated materials, but depends strongly on water content, consistent with a theoretical model of biphasic compressibility. Empirical correlations between Brillouin measurements and stiffness arise due to their mutual dependence on water content, but correlations vanish once hydration is controlled.

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http://hdl.handle.net/10453/122051