Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica

Ruiz-Campillo, MT; Molina-Hernandez, V; Escamilla, A; Stevenson, M; Perez, J; Martinez-Moreno, A; Donnelly, S; Dalton, JP; Cwiklinski, K

Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica

Ruiz-Campillo, MT Molina-Hernandez, V Escamilla, A Stevenson, M Perez, J Martinez-Moreno, A Donnelly, S

Dalton, JP Cwiklinski, K

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Publication Type:: Journal Article
Citation:: Scientific Reports, 2017, 7 (1)
Issue Date:: 2017-12-01

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Field	Value	Language
dc.contributor.author	Ruiz-Campillo, MT	en_US
dc.contributor.author	Molina-Hernandez, V	en_US
dc.contributor.author	Escamilla, A	en_US
dc.contributor.author	Stevenson, M	en_US
dc.contributor.author	Perez, J	en_US
dc.contributor.author	Martinez-Moreno, A	en_US
dc.contributor.author	Donnelly, S https://orcid.org/0000-0003-2005-3698	en_US
dc.contributor.author	Dalton, JP	en_US
dc.contributor.author	Cwiklinski, K	en_US
dc.date.available	2017-04-20	en_US
dc.date.issued	2017-12-01	en_US
dc.identifier.citation	Scientific Reports, 2017, 7 (1)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/124897
dc.description.abstract	© The Author(s) 2017. Immune signatures of sheep acutely-infected with Fasciola hepatica, an important pathogen of livestock and humans were analysed within the peritoneal compartment to investigate early infection. Within the peritoneum, F. hepatica antibodies coincided with an intense innate and adaptive cellular immune response, with infiltrating leukocytes and a marked eosinophilia (49%). However, while cytokine qPCR analysis revealed IL-10, IL-12, IL-13, IL-23 and TGFβ were elevated, these were not statistically different at 18 days post-infection compared to uninfected animals indicating that the immune response is muted and not yet skewed to a Th2 type response that is associated with chronic disease. Proteomic analysis of the peritoneal fluid identified infection-related proteins, including several structural proteins derived from the liver extracellular matrix, connective tissue and epithelium, and proteins related to the immune system. Periostin and vascular cell adhesion protein 1 (VCAM-1), molecules that mediate leukocyte infiltration and are associated with inflammatory disorders involving marked eosinophilia (e.g. asthma), were particularly elevated in the peritoneum. Immuno-histochemical studies indicated that the source of periostin and VCAM-1 was the inflamed sheep liver tissue. This study has revealed previously unknown aspects of the immunology and pathogenesis associated with acute fascioliasis in the peritoneum and liver.	en_US
dc.relation.ispartof	Scientific Reports	en_US
dc.relation.isbasedon	10.1038/s41598-017-03094-0	en_US
dc.subject.mesh	Peritoneum	en_US
dc.subject.mesh	Liver	en_US
dc.subject.mesh	Extracellular Matrix	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Sheep	en_US
dc.subject.mesh	Fasciola hepatica	en_US
dc.subject.mesh	Fascioliasis	en_US
dc.subject.mesh	Sheep Diseases	en_US
dc.subject.mesh	Inflammation Mediators	en_US
dc.subject.mesh	Cytokines	en_US
dc.subject.mesh	Biopsy	en_US
dc.subject.mesh	Leukocyte Count	en_US
dc.subject.mesh	Gene Expression Profiling	en_US
dc.subject.mesh	Biomarkers	en_US
dc.title	Immune signatures of pathogenesis in the peritoneal compartment during early infection of sheep with Fasciola hepatica	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	7	en_US
utslib.for	1107 Immunology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	7	en_US

Abstract:

© The Author(s) 2017. Immune signatures of sheep acutely-infected with Fasciola hepatica, an important pathogen of livestock and humans were analysed within the peritoneal compartment to investigate early infection. Within the peritoneum, F. hepatica antibodies coincided with an intense innate and adaptive cellular immune response, with infiltrating leukocytes and a marked eosinophilia (49%). However, while cytokine qPCR analysis revealed IL-10, IL-12, IL-13, IL-23 and TGFβ were elevated, these were not statistically different at 18 days post-infection compared to uninfected animals indicating that the immune response is muted and not yet skewed to a Th2 type response that is associated with chronic disease. Proteomic analysis of the peritoneal fluid identified infection-related proteins, including several structural proteins derived from the liver extracellular matrix, connective tissue and epithelium, and proteins related to the immune system. Periostin and vascular cell adhesion protein 1 (VCAM-1), molecules that mediate leukocyte infiltration and are associated with inflammatory disorders involving marked eosinophilia (e.g. asthma), were particularly elevated in the peritoneum. Immuno-histochemical studies indicated that the source of periostin and VCAM-1 was the inflamed sheep liver tissue. This study has revealed previously unknown aspects of the immunology and pathogenesis associated with acute fascioliasis in the peritoneum and liver.

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http://hdl.handle.net/10453/124897