Fabrication and characterization of low-cost, bead-free, durable and hydrophobic electrospun membrane for 3D cell culture
- Publication Type:
- Journal Article
- Citation:
- Biomedical Microdevices, 2017, 19 (4)
- Issue Date:
- 2017-12-01
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| Filename | Description | Size | |||
|---|---|---|---|---|---|
| Moghadas2017_Article_FabricationAndCharacterization.pdf | Published Version | 1.76 MB |
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© 2017, Springer Science+Business Media, LLC. This paper reports the fabrication of electrospun polydimethylsiloxane (PDMS) membranes/scaffolds that are suitable for three-dimensional (3D) cell culture. Through modification the ratio between PDMS and polymethylmethacrylate (PMMA) as carrier polymer, we report the possibility of increasing PDMS weight ratio of up to 6 for electrospinning. Increasing the PDMS content increases the fiber diameter, the pore size, and the hydrophobicity. To our best knowledge, this is the first report describing beads-free, durable and portable electrospun membrane with maximum content of PDMS suitable for cell culture applications. To show the proof-of-concept, we successfully cultured epithelial lung cancer cells on these membranes in a static well plate without surface modification. Surprisingly, due to three-dimensional (3D) and hydrophobic nature of the electrospun fibers, cells aggregated into 3D multicellular spheroids. These easily detachable and cost-effective scaffolds with controllable thicknesses and high tensile strength are good candidates for cell-stretching devices, organ-on-a-chip devices, tissue engineering and studies of non-adherent mammalian cancer stem cells.
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