Applications of liquid chromatography-inductively coupled plasma-mass spectrometry in the biosciences: A tutorial review and recent developments

Bishop, DP; Hare, DJ; Clases, D; Doble, PA

Applications of liquid chromatography-inductively coupled plasma-mass spectrometry in the biosciences: A tutorial review and recent developments

Bishop, DP

Hare, DJ

Clases, D

Doble, PA

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Publication Type:: Journal Article
Citation:: TrAC - Trends in Analytical Chemistry, 2018, 104 pp. 11 - 21
Issue Date:: 2018-07-01

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Field	Value	Language
dc.contributor.author	Bishop, DP https://orcid.org/0000-0002-6533-4410	en_US
dc.contributor.author	Hare, DJ https://orcid.org/0000-0002-5922-7643	en_US
dc.contributor.author	Clases, D https://orcid.org/0000-0003-3880-9385	en_US
dc.contributor.author	Doble, PA https://orcid.org/0000-0002-8472-1301	en_US
dc.date.issued	2018-07-01	en_US
dc.identifier.citation	TrAC - Trends in Analytical Chemistry, 2018, 104 pp. 11 - 21	en_US
dc.identifier.issn	0165-9936	en_US
dc.identifier.uri	http://hdl.handle.net/10453/128227
dc.description.abstract	© 2017 The biological function of minor and trace elements is ordinarily determined by their association with specific proteins, peptides and other biomolecules. Therefore, measuring the total elemental content of a biological sample provides limited information, particularly when a specific effect is due to an individual metal-protein complex. Speciation of metalloproteins, heteroatom-containing molecules or other compounds tagged with an exogenous metal can be used to overcome this limitation. A range of chromatographic separation techniques with on-line elemental detection using inductively coupled plasma-mass spectrometry (ICP-MS) have been applied to the biosciences, and each technique has intrinsic features that must be considered when designing speciation experiments. This tutorial review provides an overview of speciation in the biosciences, highlighting the unique abilities and limitations encountered. A selection of recent technical advances and new applications, the challenges of sample preparation and implementation of new technical developments are discussed, as well as the future directions of technology that is rapidly gaining a foothold in the contemporary biochemistry laboratory.	en_US
dc.relation	http://purl.org/au-research/grants/arc/LP120200079
dc.relation.ispartof	TrAC - Trends in Analytical Chemistry	en_US
dc.relation.isbasedon	10.1016/j.trac.2017.09.017	en_US
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject.classification	Analytical Chemistry	en_US
dc.title	Applications of liquid chromatography-inductively coupled plasma-mass spectrometry in the biosciences: A tutorial review and recent developments	en_US
dc.type	Journal Article
utslib.citation.volume	104	en_US
utslib.for	0301 Analytical Chemistry	en_US
utslib.for	0399 Other Chemical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
utslib.copyright.status	in_progress	*
pubs.publication-status	Published	en_US
pubs.volume	104	en_US

Abstract:

© 2017 The biological function of minor and trace elements is ordinarily determined by their association with specific proteins, peptides and other biomolecules. Therefore, measuring the total elemental content of a biological sample provides limited information, particularly when a specific effect is due to an individual metal-protein complex. Speciation of metalloproteins, heteroatom-containing molecules or other compounds tagged with an exogenous metal can be used to overcome this limitation. A range of chromatographic separation techniques with on-line elemental detection using inductively coupled plasma-mass spectrometry (ICP-MS) have been applied to the biosciences, and each technique has intrinsic features that must be considered when designing speciation experiments. This tutorial review provides an overview of speciation in the biosciences, highlighting the unique abilities and limitations encountered. A selection of recent technical advances and new applications, the challenges of sample preparation and implementation of new technical developments are discussed, as well as the future directions of technology that is rapidly gaining a foothold in the contemporary biochemistry laboratory.

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http://hdl.handle.net/10453/128227