A Comparison of Two Ovine Lumbar Intervertebral Disc Injury Models for the Evaluation and Development of Novel Regenerative Therapies

Daly, CD; Ghosh, P; Badal, T; Shimmon, R; Jenkin, G; Oehme, D; Cooper-White, J; Sher, I; Chandra, RV; Goldschlager, T

A Comparison of Two Ovine Lumbar Intervertebral Disc Injury Models for the Evaluation and Development of Novel Regenerative Therapies

Daly, CD Ghosh, P Badal, T Shimmon, R

Jenkin, G Oehme, D Cooper-White, J Sher, I Chandra, RV Goldschlager, T

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Publication Type:: Journal Article
Citation:: Global Spine Journal, 2018, 8 (8), pp. 847 - 859
Issue Date:: 2018-12-01

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Field	Value	Language
dc.contributor.author	Daly, CD	en_US
dc.contributor.author	Ghosh, P	en_US
dc.contributor.author	Badal, T	en_US
dc.contributor.author	Shimmon, R https://orcid.org/0000-0002-8763-991X	en_US
dc.contributor.author	Jenkin, G	en_US
dc.contributor.author	Oehme, D	en_US
dc.contributor.author	Cooper-White, J	en_US
dc.contributor.author	Sher, I	en_US
dc.contributor.author	Chandra, RV	en_US
dc.contributor.author	Goldschlager, T	en_US
dc.date.issued	2018-12-01	en_US
dc.identifier.citation	Global Spine Journal, 2018, 8 (8), pp. 847 - 859	en_US
dc.identifier.issn	2192-5682	en_US
dc.identifier.uri	http://hdl.handle.net/10453/129125
dc.description.abstract	© The Author(s) 2018. Study Design: Large animal research. Objective: Lumbar discectomy is the most commonly performed spinal surgical procedure. We investigated 2 large animal models of lumbar discectomy in order to study the regenerative capacity of mesenchymal stem cells following disc injury. Methods: Twelve adult ewes underwent baseline 3-T magnetic resonance imaging (MRI) followed by lumbar intervertebral disc injury by either drill bit (n = 6) or annulotomy and partial nucleotomy (APN) (n = 6). Necropsies were performed 6 months later. Lumbar spines underwent 3-T and 9.4-T MRI prior to histological, morphological and biochemical analysis. Results: Drill bit-injured (DBI) and APN-injured discs demonstrated increased Pfirrmann grades relative to uninjured controls (P <.005), with no difference between the 2 models. Disc height index loss was greater in the APN group compared with the DBI group (P <.005). Gross morphology injury scores were higher in APN than DBI discs (P <.05) and both were higher than controls (P <.005). Proteoglycan was reduced in the discs of both injury models relative to controls (P <.005), but lower in the APN group (P <.05). Total collagen of the APN group disc regions was higher than DBI and control discs (P <.05). Histology revealed more matrix degeneration, vascular infiltration, and granulation in the APN model. Conclusion: Although both models produced disc degeneration, the APN model better replicated the pathobiology of human discs postdiscectomy. We therefore concluded that the APN model was a more appropriate model for the investigation of the regenerative capacity of mesenchymal stem cells administered postdiscectomy.	en_US
dc.relation.ispartof	Global Spine Journal	en_US
dc.relation.isbasedon	10.1177/2192568218779988	en_US
dc.title	A Comparison of Two Ovine Lumbar Intervertebral Disc Injury Models for the Evaluation and Development of Novel Regenerative Therapies	en_US
dc.type	Journal Article
utslib.citation.volume	8	en_US
utslib.for	0603 Evolutionary Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CCET - Centre for Clean Energy Technology
utslib.copyright.status	open_access
pubs.issue	8	en_US
pubs.publication-status	Published	en_US
pubs.volume	8	en_US

Abstract:

© The Author(s) 2018. Study Design: Large animal research. Objective: Lumbar discectomy is the most commonly performed spinal surgical procedure. We investigated 2 large animal models of lumbar discectomy in order to study the regenerative capacity of mesenchymal stem cells following disc injury. Methods: Twelve adult ewes underwent baseline 3-T magnetic resonance imaging (MRI) followed by lumbar intervertebral disc injury by either drill bit (n = 6) or annulotomy and partial nucleotomy (APN) (n = 6). Necropsies were performed 6 months later. Lumbar spines underwent 3-T and 9.4-T MRI prior to histological, morphological and biochemical analysis. Results: Drill bit-injured (DBI) and APN-injured discs demonstrated increased Pfirrmann grades relative to uninjured controls (P <.005), with no difference between the 2 models. Disc height index loss was greater in the APN group compared with the DBI group (P <.005). Gross morphology injury scores were higher in APN than DBI discs (P <.05) and both were higher than controls (P <.005). Proteoglycan was reduced in the discs of both injury models relative to controls (P <.005), but lower in the APN group (P <.05). Total collagen of the APN group disc regions was higher than DBI and control discs (P <.05). Histology revealed more matrix degeneration, vascular infiltration, and granulation in the APN model. Conclusion: Although both models produced disc degeneration, the APN model better replicated the pathobiology of human discs postdiscectomy. We therefore concluded that the APN model was a more appropriate model for the investigation of the regenerative capacity of mesenchymal stem cells administered postdiscectomy.

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http://hdl.handle.net/10453/129125