The effects of soaking for DNA recovery on the striation patterns of fired cartridge cases

Prasad, E; Van der Walt, L; Cole, A; van Oorschot, RAH; Barash, M; Gunn, P; Raymond, J

The effects of soaking for DNA recovery on the striation patterns of fired cartridge cases

Prasad, E Van der Walt, L Cole, A van Oorschot, RAH Barash, M

Gunn, P

Raymond, J

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Publication Type:: Journal Article
Citation:: Australian Journal of Forensic Sciences, 2019, 51 (sup1), pp. S35 - S38
Issue Date:: 2019-07-29

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Field	Value	Language
dc.contributor.author	Prasad, E	en_US
dc.contributor.author	Van der Walt, L	en_US
dc.contributor.author	Cole, A	en_US
dc.contributor.author	van Oorschot, RAH	en_US
dc.contributor.author	Barash, M https://orcid.org/0000-0002-5445-6316	en_US
dc.contributor.author	Gunn, P https://orcid.org/0000-0002-3987-0447	en_US
dc.contributor.author	Raymond, J	en_US
dc.date.available	2020-05-25T19:08:33Z
dc.date.issued	2019-07-29	en_US
dc.identifier.citation	Australian Journal of Forensic Sciences, 2019, 51 (sup1), pp. S35 - S38	en_US
dc.identifier.issn	0045-0618	en_US
dc.identifier.uri	http://hdl.handle.net/10453/130535
dc.description.abstract	© 2019, © 2019 Australian Academy of Forensic Sciences. The recovery of trace DNA from fired cartridge cases has recently gained increased interest throughout the literature, with a variety of methods currently being explored. Soaking fired cartridge cases in a lysis buffer holds potential in producing meaningful DNA profiles; however, chemical interactions between the lysis buffer and brass cartridge cases may limit the efficacy of this method. This preliminary study examines the effects of soaking on the microscopic striation detail of brass and nickel 9 mm Parabellum (9 mmP) calibre and.22 Long Rifle (.22LR) calibre fired cartridge cases. Headstamp and coarse striation patterns on 9 mmP fired cartridge cases and finer striation patterns along the outer wall of.22LR fired cartridge cases were microscopically examined prior to and following soaking. Soaking was performed by submerging the fired cartridge cases in 380 µl of ATL buffer (Qiagen, Germany) for 20 minutes. Microscopic analysis of brass and nickel 9 mmP and.22LR fired cartridge cases showed that coarse and fine striation detail remain unaffected following soaking. These results indicate that comparative ballistics examinations may be performed following DNA recovery using the soaking method.	en_US
dc.relation.ispartof	Australian Journal of Forensic Sciences	en_US
dc.relation.isbasedon	10.1080/00450618.2019.1569144	en_US
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.classification	Legal & Forensic Medicine	en_US
dc.title	The effects of soaking for DNA recovery on the striation patterns of fired cartridge cases	en_US
dc.type	Journal Article
utslib.citation.volume	sup1	en_US
utslib.citation.volume	51	en_US
utslib.for	0699 Other Biological Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	open_access	*
pubs.issue	sup1	en_US
pubs.publication-status	Published	en_US
pubs.volume	51	en_US

Abstract:

© 2019, © 2019 Australian Academy of Forensic Sciences. The recovery of trace DNA from fired cartridge cases has recently gained increased interest throughout the literature, with a variety of methods currently being explored. Soaking fired cartridge cases in a lysis buffer holds potential in producing meaningful DNA profiles; however, chemical interactions between the lysis buffer and brass cartridge cases may limit the efficacy of this method. This preliminary study examines the effects of soaking on the microscopic striation detail of brass and nickel 9 mm Parabellum (9 mmP) calibre and.22 Long Rifle (.22LR) calibre fired cartridge cases. Headstamp and coarse striation patterns on 9 mmP fired cartridge cases and finer striation patterns along the outer wall of.22LR fired cartridge cases were microscopically examined prior to and following soaking. Soaking was performed by submerging the fired cartridge cases in 380 µl of ATL buffer (Qiagen, Germany) for 20 minutes. Microscopic analysis of brass and nickel 9 mmP and.22LR fired cartridge cases showed that coarse and fine striation detail remain unaffected following soaking. These results indicate that comparative ballistics examinations may be performed following DNA recovery using the soaking method.

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http://hdl.handle.net/10453/130535