Pandoraea fibrosis sp. Nov., a novel pandoraea species isolated from clinical respiratory samples

See-Too, WS; Ambrose, M; Malley, R; Ee, R; Mulcahy, E; Manche, E; Lazenby, J; McEwan, B; Pagnon, J; Chen, JW; Chan, KG; Turnbull, L; Whitchurch, CB; Roddam, LF

Pandoraea fibrosis sp. Nov., a novel pandoraea species isolated from clinical respiratory samples

See-Too, WS Ambrose, M Malley, R Ee, R Mulcahy, E Manche, E Lazenby, J

McEwan, B Pagnon, J Chen, JW Chan, KG Turnbull, L

Whitchurch, CB

Roddam, LF

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Publication Type:: Journal Article
Citation:: International Journal of Systematic and Evolutionary Microbiology, 2019, 69 (3), pp. 645 - 651
Issue Date:: 2019-03-01

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Field	Value	Language
dc.contributor.author	See-Too, WS	en_US
dc.contributor.author	Ambrose, M	en_US
dc.contributor.author	Malley, R	en_US
dc.contributor.author	Ee, R	en_US
dc.contributor.author	Mulcahy, E	en_US
dc.contributor.author	Manche, E	en_US
dc.contributor.author	Lazenby, J https://orcid.org/0000-0001-6198-2640	en_US
dc.contributor.author	McEwan, B	en_US
dc.contributor.author	Pagnon, J	en_US
dc.contributor.author	Chen, JW	en_US
dc.contributor.author	Chan, KG	en_US
dc.contributor.author	Turnbull, L https://orcid.org/0000-0002-9255-9033	en_US
dc.contributor.author	Whitchurch, CB https://orcid.org/0000-0003-2296-3791	en_US
dc.contributor.author	Roddam, LF	en_US
dc.date.issued	2019-03-01	en_US
dc.identifier.citation	International Journal of Systematic and Evolutionary Microbiology, 2019, 69 (3), pp. 645 - 651	en_US
dc.identifier.issn	1466-5026	en_US
dc.identifier.uri	http://hdl.handle.net/10453/130653
dc.description.abstract	© 2019 IUMS. Pandoraea species have been isolated from diverse environmental samples and are emerging important respiratory pathogens, particularly in people with cystic fibrosis (CF). In the present study, two bacterial isolates initially recovered from consecutive sputum samples collected from a CF patient and identified as Pandoraea pnomenusa underwent a polyphasic taxonomic analysis. The isolates were found to be Gram-negative, facultative anaerobic motile bacilli and subsequently designated as strains 6399 T (=LMG29626 T =DSM103228 T ) and 7641 (=LMG29627=DSM103229), respectively. Phylogenetic analysis based on 16S rRNA and gyrB gene sequences revealed that 6399 T and 7641 formed a distinct phylogenetic lineage within the genus Pandoraea. Genome sequence comparison analysis indicated that strains 6399 T and 7641 are clonal and share 100 % similarity, however, similarity to other type strains (ANIb 73.2–88.8 %, ANIm 83.5–89.9 % and OrthoANI 83.2– 89.3 %) indicates that 6399 T and 7641 do not belong to any of the reported type species. The major cellular fatty acids of 6399 T were C 16: 0 (32.1 %) C 17: 0 cyclo (18.7 %) and C 18: 1 !7c (14.5 %), while Q-8 was the only respiratory quinone detected. The major polar lipids identified were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G+C content of 6399 T was 62.9 (mol%). Strain 6399 T can be differentiated from other members of Pandoraea by the absence of C 19: 0 !8c cyclo and by the presence of C 17: 0 !8c cyclo. Together our data show that the bacterial strains 6399 T and 7641 represent a novel species of the genus Pandoraea, for which the name Pandoraea fibrosis sp. nov. is proposed (type strain 6399 T ).	en_US
dc.relation.ispartof	International Journal of Systematic and Evolutionary Microbiology	en_US
dc.relation.isbasedon	10.1099/ijsem.0.003147	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Sputum	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Burkholderiaceae	en_US
dc.subject.mesh	Cystic Fibrosis	en_US
dc.subject.mesh	Ubiquinone	en_US
dc.subject.mesh	Fatty Acids	en_US
dc.subject.mesh	Phospholipids	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	RNA, Ribosomal, 16S	en_US
dc.subject.mesh	Bacterial Typing Techniques	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Base Composition	en_US
dc.subject.mesh	Genes, Bacterial	en_US
dc.subject.mesh	Tasmania	en_US
dc.title	Pandoraea fibrosis sp. Nov., a novel pandoraea species isolated from clinical respiratory samples	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	69	en_US
utslib.for	0603 Evolutionary Biology	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	1108 Medical Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	69	en_US

Abstract:

© 2019 IUMS. Pandoraea species have been isolated from diverse environmental samples and are emerging important respiratory pathogens, particularly in people with cystic fibrosis (CF). In the present study, two bacterial isolates initially recovered from consecutive sputum samples collected from a CF patient and identified as Pandoraea pnomenusa underwent a polyphasic taxonomic analysis. The isolates were found to be Gram-negative, facultative anaerobic motile bacilli and subsequently designated as strains 6399 T (=LMG29626 T =DSM103228 T ) and 7641 (=LMG29627=DSM103229), respectively. Phylogenetic analysis based on 16S rRNA and gyrB gene sequences revealed that 6399 T and 7641 formed a distinct phylogenetic lineage within the genus Pandoraea. Genome sequence comparison analysis indicated that strains 6399 T and 7641 are clonal and share 100 % similarity, however, similarity to other type strains (ANIb 73.2–88.8 %, ANIm 83.5–89.9 % and OrthoANI 83.2– 89.3 %) indicates that 6399 T and 7641 do not belong to any of the reported type species. The major cellular fatty acids of 6399 T were C 16: 0 (32.1 %) C 17: 0 cyclo (18.7 %) and C 18: 1 !7c (14.5 %), while Q-8 was the only respiratory quinone detected. The major polar lipids identified were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G+C content of 6399 T was 62.9 (mol%). Strain 6399 T can be differentiated from other members of Pandoraea by the absence of C 19: 0 !8c cyclo and by the presence of C 17: 0 !8c cyclo. Together our data show that the bacterial strains 6399 T and 7641 represent a novel species of the genus Pandoraea, for which the name Pandoraea fibrosis sp. nov. is proposed (type strain 6399 T ).

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http://hdl.handle.net/10453/130653