Ovarian Hyperstimulation Reduces Vascular Endothelial Growth Factor-A During Uterine Receptivity

Danastas, K; Whittington, CM; Dowland, SN; Combes, V; Murphy, CR; Lindsay, LA

Ovarian Hyperstimulation Reduces Vascular Endothelial Growth Factor-A During Uterine Receptivity

Danastas, K Whittington, CM Dowland, SN Combes, V

Murphy, CR Lindsay, LA

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Publication Type:: Journal Article
Citation:: Reproductive Sciences, 2019, 26 (2), pp. 259 - 268
Issue Date:: 2019-02-01

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Field	Value	Language
dc.contributor.author	Danastas, K	en_US
dc.contributor.author	Whittington, CM	en_US
dc.contributor.author	Dowland, SN	en_US
dc.contributor.author	Combes, V https://orcid.org/0000-0003-2178-3596	en_US
dc.contributor.author	Murphy, CR	en_US
dc.contributor.author	Lindsay, LA	en_US
dc.date.issued	2019-02-01	en_US
dc.identifier.citation	Reproductive Sciences, 2019, 26 (2), pp. 259 - 268	en_US
dc.identifier.issn	1933-7191	en_US
dc.identifier.uri	http://hdl.handle.net/10453/130772
dc.description.abstract	© The Author(s) 2018. The angiogenic factor vascular endothelial growth factor-A (VEGFA) plays a critical role during early pregnancy in many species including the rat, and any alterations in VEGFA levels can severely impact blastocyst implantation rates. The rat ovarian hyperstimulation (OH) model is useful in studying how the induction of superovulation affects VEGFA levels and endometrial receptivity to blastocyst implantation. The present study shows that the major isoform in the rat uterus, Vegf 188 , is reduced at the time of receptivity in OH compared to normal pregnancy, whereas there is no change in Vegf 164 and Vegf 120 messenger RNA (mRNA). The VEGFA receptor 2 (VEGFR2) protein levels are also reduced at the time of receptivity in OH. Our ovariectomy studies show that Vegf 164 , Vegf 188 , and Vegf 120 are significantly decreased by estrogen, and, to a lesser extent progesterone, when compared to control animals. Although no change in the percentage of endometrial blood vessels was seen across all stages of pregnancy, at the time of receptivity in OH pregnancies, blood vessels were typically larger compared to other stages. The altered progesterone–estrogen ratio seen in OH, taken together with our ovariectomy studies, explains the changes to Vegfa mRNA in OH at the time of receptivity. Since VEGFA is important during implantation, the changes to Vegfa and VEGFR2 levels in the endometrium may help explain the observed lower endometrial receptivity following OH. This study aimed to analyse how ovarian hyperstimulation alters the levels of vascular endothleial growth factor and its major receptor, VEGFR2 in the uterus in a rat model.	en_US
dc.relation.ispartof	Reproductive Sciences	en_US
dc.relation.isbasedon	10.1177/1933719118768703	en_US
dc.subject.classification	Obstetrics & Reproductive Medicine	en_US
dc.subject.mesh	Uterus	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Rats	en_US
dc.subject.mesh	Rats, Wistar	en_US
dc.subject.mesh	Ovarian Hyperstimulation Syndrome	en_US
dc.subject.mesh	Estradiol	en_US
dc.subject.mesh	Progesterone	en_US
dc.subject.mesh	Vascular Endothelial Growth Factor A	en_US
dc.subject.mesh	Ovulation Induction	en_US
dc.subject.mesh	Female	en_US
dc.title	Ovarian Hyperstimulation Reduces Vascular Endothelial Growth Factor-A During Uterine Receptivity	en_US
dc.type	Journal Article
utslib.citation.volume	2	en_US
utslib.citation.volume	26	en_US
utslib.for	1114 Paediatrics and Reproductive Medicine	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	2	en_US
pubs.publication-status	Published	en_US
pubs.volume	26	en_US

Abstract:

© The Author(s) 2018. The angiogenic factor vascular endothelial growth factor-A (VEGFA) plays a critical role during early pregnancy in many species including the rat, and any alterations in VEGFA levels can severely impact blastocyst implantation rates. The rat ovarian hyperstimulation (OH) model is useful in studying how the induction of superovulation affects VEGFA levels and endometrial receptivity to blastocyst implantation. The present study shows that the major isoform in the rat uterus, Vegf 188 , is reduced at the time of receptivity in OH compared to normal pregnancy, whereas there is no change in Vegf 164 and Vegf 120 messenger RNA (mRNA). The VEGFA receptor 2 (VEGFR2) protein levels are also reduced at the time of receptivity in OH. Our ovariectomy studies show that Vegf 164 , Vegf 188 , and Vegf 120 are significantly decreased by estrogen, and, to a lesser extent progesterone, when compared to control animals. Although no change in the percentage of endometrial blood vessels was seen across all stages of pregnancy, at the time of receptivity in OH pregnancies, blood vessels were typically larger compared to other stages. The altered progesterone–estrogen ratio seen in OH, taken together with our ovariectomy studies, explains the changes to Vegfa mRNA in OH at the time of receptivity. Since VEGFA is important during implantation, the changes to Vegfa and VEGFR2 levels in the endometrium may help explain the observed lower endometrial receptivity following OH. This study aimed to analyse how ovarian hyperstimulation alters the levels of vascular endothleial growth factor and its major receptor, VEGFR2 in the uterus in a rat model.

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http://hdl.handle.net/10453/130772