Combined imaging of bacteria and oxygen in biofilms

Kühl, M; Rickelt, LF; Thar, R

Combined imaging of bacteria and oxygen in biofilms

Kühl, M

Rickelt, LF Thar, R

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Publication Type:: Journal Article
Citation:: Applied and Environmental Microbiology, 2007, 73 (19), pp. 6289 - 6295
Issue Date:: 2007-10-01

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Field	Value	Language
dc.contributor.author	Kühl, M https://orcid.org/0000-0002-1792-4790	en_US
dc.contributor.author	Rickelt, LF	en_US
dc.contributor.author	Thar, R	en_US
dc.date.issued	2007-10-01	en_US
dc.identifier.citation	Applied and Environmental Microbiology, 2007, 73 (19), pp. 6289 - 6295	en_US
dc.identifier.issn	0099-2240	en_US
dc.identifier.uri	http://hdl.handle.net/10453/13306
dc.description.abstract	Transparent sensors for microscopic O2 imaging were developed by spin coating an ultrathin (<1- to 2-μM) layer of a luminescent O 2 indicator onto coverslips. The sensors showed (i) an ideal Stern-Volmer quenching behavior of the luminescence lifetime towards O 2 levels, (ii) homogeneous measuring characteristics over the sensor surface, and (iii) a linear decline of luminescence lifetime with increasing temperature. When a batch of such coverslip sensors has been characterized, their use is thus essentially calibration free at a known temperature. The sensors are easy to use in flow chambers and other growth devices used in microbiology. We present the first application for combined imaging of O 2 and bacteria in a biofilm flow chamber mounted on a microscope equipped with a spinning-disk confocal unit and a luminescence lifetime camera system. Copyright © 2007, American Society for Microbiology. All Rights Reserved.	en_US
dc.relation.ispartof	Applied and Environmental Microbiology	en_US
dc.relation.isbasedon	10.1128/AEM.01574-07	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Bacteria	en_US
dc.subject.mesh	Biofilms	en_US
dc.subject.mesh	Oxygen	en_US
dc.subject.mesh	Microscopy, Confocal	en_US
dc.subject.mesh	Luminescent Measurements	en_US
dc.subject.mesh	Biosensing Techniques	en_US
dc.subject.mesh	Electronics	en_US
dc.title	Combined imaging of bacteria and oxygen in biofilms	en_US
dc.type	Journal Article
utslib.citation.volume	19	en_US
utslib.citation.volume	73	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0602 Ecology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	19	en_US
pubs.publication-status	Published	en_US
pubs.volume	73	en_US

Abstract:

Transparent sensors for microscopic O2 imaging were developed by spin coating an ultrathin (<1- to 2-μM) layer of a luminescent O 2 indicator onto coverslips. The sensors showed (i) an ideal Stern-Volmer quenching behavior of the luminescence lifetime towards O 2 levels, (ii) homogeneous measuring characteristics over the sensor surface, and (iii) a linear decline of luminescence lifetime with increasing temperature. When a batch of such coverslip sensors has been characterized, their use is thus essentially calibration free at a known temperature. The sensors are easy to use in flow chambers and other growth devices used in microbiology. We present the first application for combined imaging of O 2 and bacteria in a biofilm flow chamber mounted on a microscope equipped with a spinning-disk confocal unit and a luminescence lifetime camera system. Copyright © 2007, American Society for Microbiology. All Rights Reserved.

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http://hdl.handle.net/10453/13306