Different bacterial communities associated with the roots and bulk sediment of the seagrass Zostera marina

Jensen, SI; Kühl, M; Priemé, A

Different bacterial communities associated with the roots and bulk sediment of the seagrass Zostera marina

Jensen, SI Kühl, M

Priemé, A

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Publication Type:: Journal Article
Citation:: FEMS Microbiology Ecology, 2007, 62 (1), pp. 108 - 117
Issue Date:: 2007-10-01

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Field	Value	Language
dc.contributor.author	Jensen, SI	en_US
dc.contributor.author	Kühl, M https://orcid.org/0000-0002-1792-4790	en_US
dc.contributor.author	Priemé, A	en_US
dc.date.issued	2007-10-01	en_US
dc.identifier.citation	FEMS Microbiology Ecology, 2007, 62 (1), pp. 108 - 117	en_US
dc.identifier.issn	0168-6496	en_US
dc.identifier.uri	http://hdl.handle.net/10453/13307
dc.description.abstract	The bacterial community of Zostera marina-inhabited bulk sediment vs. root-associated bacteria was investigated by terminal restriction fragment length polymorphism and sequencing, and the spatial extension of the oxygen loss from roots was determined by oxygen microsensors. Extensive oxygen loss was found in the tip region of the youngest roots, and most of the rhizoplane of Z. marina roots was thus anoxic. A significant difference between the bacterial communities associated with the roots and bulk sediment was found. No significant differences were found between differently aged root-bundles. Terminal restriction fragments (TRFs) assigned to sulfate-reducing Deltaproteobacteria showed a relative mean distribution of 12% and 23% of the PCR-amplified bacterial community in the bulk-sediment at the two sites, but only contributed <2% to the root-associated communities. TRFs assigned to Epsilonproteobacteria showed a relative mean distribution of between 5% and 11% in the root-associated communities of the youngest root bundle, in contrast to the bulk-sediment where this TRF only contributed <1.3%. TRFs assigned to Actinobacteria and Gammaproteobacteria also seemed important first root-colonizers, whereas TRFs assigned to Deltaproteobacteria became increasingly important in the root-associated community of the older root bundles. The presence of the roots thus apparently selects for a distinct bacterial community, stimulating the growth of potential symbiotic Epsilon- and Gammaproteobacteria and/or inhibiting the growth of sulfate-reducing Deltaproteobacteria. © 2007 Federation of European Microbiological Societies.	en_US
dc.relation.ispartof	FEMS Microbiology Ecology	en_US
dc.relation.isbasedon	10.1111/j.1574-6941.2007.00373.x	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Bacteria	en_US
dc.subject.mesh	Zosteraceae	en_US
dc.subject.mesh	Plant Roots	en_US
dc.subject.mesh	Oxygen	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	DNA, Ribosomal	en_US
dc.subject.mesh	RNA, Ribosomal, 16S	en_US
dc.subject.mesh	DNA Fingerprinting	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Ecosystem	en_US
dc.subject.mesh	Biodiversity	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Polymorphism, Restriction Fragment Length	en_US
dc.subject.mesh	Geologic Sediments	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.title	Different bacterial communities associated with the roots and bulk sediment of the seagrass Zostera marina	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	62	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	62	en_US

Abstract:

The bacterial community of Zostera marina-inhabited bulk sediment vs. root-associated bacteria was investigated by terminal restriction fragment length polymorphism and sequencing, and the spatial extension of the oxygen loss from roots was determined by oxygen microsensors. Extensive oxygen loss was found in the tip region of the youngest roots, and most of the rhizoplane of Z. marina roots was thus anoxic. A significant difference between the bacterial communities associated with the roots and bulk sediment was found. No significant differences were found between differently aged root-bundles. Terminal restriction fragments (TRFs) assigned to sulfate-reducing Deltaproteobacteria showed a relative mean distribution of 12% and 23% of the PCR-amplified bacterial community in the bulk-sediment at the two sites, but only contributed <2% to the root-associated communities. TRFs assigned to Epsilonproteobacteria showed a relative mean distribution of between 5% and 11% in the root-associated communities of the youngest root bundle, in contrast to the bulk-sediment where this TRF only contributed <1.3%. TRFs assigned to Actinobacteria and Gammaproteobacteria also seemed important first root-colonizers, whereas TRFs assigned to Deltaproteobacteria became increasingly important in the root-associated community of the older root bundles. The presence of the roots thus apparently selects for a distinct bacterial community, stimulating the growth of potential symbiotic Epsilon- and Gammaproteobacteria and/or inhibiting the growth of sulfate-reducing Deltaproteobacteria. © 2007 Federation of European Microbiological Societies.

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http://hdl.handle.net/10453/13307