Isolation and computer-aided characterization of MmeI, a type II restriction endonuclease from Methylophilus methylotrophus.

Boyd, AC; Charles, IG; Keyte, JW; Brammar, WJ

Isolation and computer-aided characterization of MmeI, a type II restriction endonuclease from Methylophilus methylotrophus.

Boyd, AC Charles, IG Keyte, JW Brammar, WJ

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Publication Type:: Journal Article
Citation:: Nucleic Acids Res, 1986, 14 (13), pp. 5255 - 5274
Issue Date:: 1986-07-11

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Field	Value	Language
dc.contributor.author	Boyd, AC	en_US
dc.contributor.author	Charles, IG	en_US
dc.contributor.author	Keyte, JW	en_US
dc.contributor.author	Brammar, WJ	en_US
dc.date.issued	1986-07-11	en_US
dc.identifier.citation	Nucleic Acids Res, 1986, 14 (13), pp. 5255 - 5274	en_US
dc.identifier.issn	0305-1048	en_US
dc.identifier.uri	http://hdl.handle.net/10453/13386
dc.description.abstract	A Type II restriction endonuclease, MmeI, has been purified from the obligate methylotroph, Methylophilus methylotrophus. The enzyme was shown to have the non-palindromic recognition sequence 5'-T C C Pu A C (N)20-3', 3'-A G G Py T G (N)18-5' and to cleave (as indicated) on the 3' side, generating a two nucleotide 3' projection. Determination of the recognition sequence was achieved using two new computer programs; RECOG, which predicts recognition sequences from the pattern of restriction fragments obtained from DNAs of known sequence, and GELSIM, which generates graphical simulations of DNA band patterns obtained by gel electrophoresis of restriction digests of sequenced DNA molecules.	en_US
dc.language	eng	en_US
dc.relation.ispartof	Nucleic Acids Res	en_US
dc.relation.isbasedon	10.1093/nar/14.13.5255	en_US
dc.subject.classification	Developmental Biology	en_US
dc.subject.mesh	Methylococcaceae	en_US
dc.subject.mesh	DNA Restriction Enzymes	en_US
dc.subject.mesh	Deoxyribonucleases, Type II Site-Specific	en_US
dc.subject.mesh	Chromosome Mapping	en_US
dc.subject.mesh	Substrate Specificity	en_US
dc.subject.mesh	Software	en_US
dc.title	Isolation and computer-aided characterization of MmeI, a type II restriction endonuclease from Methylophilus methylotrophus.	en_US
dc.type	Journal Article
utslib.citation.volume	13	en_US
utslib.citation.volume	14	en_US
utslib.location.activity	England	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	08 Information and Computing Sciences	en_US
dc.location.activity	ISI:A1986D269500010	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/DVC (Corporate Services)
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	13	en_US
pubs.publication-status	Published	en_US
pubs.volume	14	en_US

Abstract:

A Type II restriction endonuclease, MmeI, has been purified from the obligate methylotroph, Methylophilus methylotrophus. The enzyme was shown to have the non-palindromic recognition sequence 5'-T C C Pu A C (N)20-3', 3'-A G G Py T G (N)18-5' and to cleave (as indicated) on the 3' side, generating a two nucleotide 3' projection. Determination of the recognition sequence was achieved using two new computer programs; RECOG, which predicts recognition sequences from the pattern of restriction fragments obtained from DNAs of known sequence, and GELSIM, which generates graphical simulations of DNA band patterns obtained by gel electrophoresis of restriction digests of sequenced DNA molecules.

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http://hdl.handle.net/10453/13386