Use of a Synthetic Oligonucleotide to Identify a Chromosomal Gene for Chloramphenicol Acetyltransferase in a Plasmid-bearing Flavobacterium

Beschle, HG; Charles, IG; Shaw, WV

Use of a Synthetic Oligonucleotide to Identify a Chromosomal Gene for Chloramphenicol Acetyltransferase in a Plasmid-bearing Flavobacterium

Beschle, HG Charles, IG Shaw, WV

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Publisher:: Soc General Microbiology
Publication Type:: Journal Article
Citation:: Journal of General Microbiology, 1984, 130 (NA), pp. 3335 - 3338
Issue Date:: 1984-01

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Field	Value	Language
dc.contributor.author	Beschle, HG	en_US
dc.contributor.author	Charles, IG	en_US
dc.contributor.author	Shaw, WV	en_US
dc.date.issued	1984-01	en_US
dc.identifier.citation	Journal of General Microbiology, 1984, 130 (NA), pp. 3335 - 3338	en_US
dc.identifier.issn	0022-1287	en_US
dc.identifier.uri	http://hdl.handle.net/10453/13410
dc.description.abstract	A micro-organism previously designated Flavobacterium sp. CB60 is resistant to chloramphenicol as a consequence of antibiotic acetylation by the enzyme chloramphenicol acetyltransferase and subsequent degradation of the acetylated product by co-metabolism. Although a 15.6 kb plasmid (pCB60) was demonstrated in this Flavobacterium strain, it did not appear to play a role in chloramphenicol acetylation. DNA hybridization was used to identify a fragment of DNA presumptively carrying the cat gene. The sequence of the synthetic probe was based upon known nucleotide sequences corresponding to the highly-conserved active site region of several chloramphenicol acetyltransferase variants. The structural gene for the enzyme in strain CB60 appeared to be chromosomal since the radioactive probe hybridized with a unique restriction fragment from chromosomal DNA but failed to do so with the DNA of plasmid pCB60.	en_US
dc.publisher	Soc General Microbiology	en_US
dc.relation.ispartof	Journal of General Microbiology	en_US
dc.title	Use of a Synthetic Oligonucleotide to Identify a Chromosomal Gene for Chloramphenicol Acetyltransferase in a Plasmid-bearing Flavobacterium	en_US
dc.type	Journal Article
utslib.citation.volume	NA	en_US
utslib.citation.volume	130	en_US
utslib.for	0605 Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.consider-herdc	false	en_US
pubs.issue	NA	en_US
pubs.volume	130	en_US

Abstract:

A micro-organism previously designated Flavobacterium sp. CB60 is resistant to chloramphenicol as a consequence of antibiotic acetylation by the enzyme chloramphenicol acetyltransferase and subsequent degradation of the acetylated product by co-metabolism. Although a 15.6 kb plasmid (pCB60) was demonstrated in this Flavobacterium strain, it did not appear to play a role in chloramphenicol acetylation. DNA hybridization was used to identify a fragment of DNA presumptively carrying the cat gene. The sequence of the synthetic probe was based upon known nucleotide sequences corresponding to the highly-conserved active site region of several chloramphenicol acetyltransferase variants. The structural gene for the enzyme in strain CB60 appeared to be chromosomal since the radioactive probe hybridized with a unique restriction fragment from chromosomal DNA but failed to do so with the DNA of plasmid pCB60.

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http://hdl.handle.net/10453/13410