A diagnostic PCR assay for the detection of an Australian epidemic strain of Pseudomonas aeruginosa

Williams, HL; Turnbull, L; Thomas, SJ; Murphy, A; Stinear, T; Armstrong, DS; Whitchurch, CB

A diagnostic PCR assay for the detection of an Australian epidemic strain of Pseudomonas aeruginosa

Williams, HL Turnbull, L

Thomas, SJ Murphy, A Stinear, T Armstrong, DS Whitchurch, CB

Permalink

Publisher:: BioMed Central Ltd
Publication Type:: Journal Article
Citation:: Annals of Clinical Microbiology and Antimicrobials, 2010, 9 (18), pp. 1 - 8
Issue Date:: 2010-01

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download full textAdobe PDF (709.8 kB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Williams, HL	en_US
dc.contributor.author	Turnbull, L https://orcid.org/0000-0002-9255-9033	en_US
dc.contributor.author	Thomas, SJ	en_US
dc.contributor.author	Murphy, A	en_US
dc.contributor.author	Stinear, T	en_US
dc.contributor.author	Armstrong, DS	en_US
dc.contributor.author	Whitchurch, CB https://orcid.org/0000-0003-2296-3791	en_US
dc.date.issued	2010-01	en_US
dc.identifier.citation	Annals of Clinical Microbiology and Antimicrobials, 2010, 9 (18), pp. 1 - 8	en_US
dc.identifier.issn	1476-0711	en_US
dc.identifier.uri	http://hdl.handle.net/10453/13814
dc.description.abstract	Background Chronic lung infection with the bacterium Pseudomonas aeruginosa is one of the hallmarks of cystic fibrosis (CF) and is associated with worsening lung function, increased hospitalisation and reduced life expectancy. A virulent clonal strain of P. aeruginosa (Australian epidemic strain I; AES-I) has been found to be widespread in CF patients in eastern Australia. Methods Suppression subtractive hybridization (SSH) was employed to identify genetic sequences that are present in the AES-I strain but absent from the sequenced reference strain PAO1. We used PCR to evaluate the distribution of several of the AES-I loci amongst a collection of 188 P. aeruginosa isolates which was comprised of 35 AES-I isolates (as determined by PFGE), 78 non-AES-I CF isolates including other epidemic CF strains as well as 69 P. aeruginosa isolates from other clinical and environmental sources. Results We have identified a unique AES-I genetic locus that is present in all 35 AES-I isolates tested and not present in any of the other 153 P. aeruginosa strains examined. We have used this unique AES-I locus to develop a diagnostic PCR and a real-time PCR assay to detect the presence of P. aeruginosa and AES-I in patient sputum samples.	en_US
dc.publisher	BioMed Central Ltd	en_US
dc.relation.ispartof	Annals of Clinical Microbiology and Antimicrobials	en_US
dc.relation.isbasedon	10.1111/j.1469-0691.2010.03439.x	en_US
dc.subject.classification	Microbiology	en_US
dc.title	A diagnostic PCR assay for the detection of an Australian epidemic strain of Pseudomonas aeruginosa	en_US
dc.type	Journal Article
utslib.citation.volume	18	en_US
utslib.citation.volume	9	en_US
utslib.for	1103 Clinical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.consider-herdc	true	en_US
pubs.issue	18	en_US
pubs.publication-status	Published	en_US
pubs.volume	9	en_US

Abstract:

Background Chronic lung infection with the bacterium Pseudomonas aeruginosa is one of the hallmarks of cystic fibrosis (CF) and is associated with worsening lung function, increased hospitalisation and reduced life expectancy. A virulent clonal strain of P. aeruginosa (Australian epidemic strain I; AES-I) has been found to be widespread in CF patients in eastern Australia. Methods Suppression subtractive hybridization (SSH) was employed to identify genetic sequences that are present in the AES-I strain but absent from the sequenced reference strain PAO1. We used PCR to evaluate the distribution of several of the AES-I loci amongst a collection of 188 P. aeruginosa isolates which was comprised of 35 AES-I isolates (as determined by PFGE), 78 non-AES-I CF isolates including other epidemic CF strains as well as 69 P. aeruginosa isolates from other clinical and environmental sources. Results We have identified a unique AES-I genetic locus that is present in all 35 AES-I isolates tested and not present in any of the other 153 P. aeruginosa strains examined. We have used this unique AES-I locus to develop a diagnostic PCR and a real-time PCR assay to detect the presence of P. aeruginosa and AES-I in patient sputum samples.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/13814