Neurological effects in the offspring after switching from tobacco cigarettes to e-cigarettes during pregnancy in a mouse model.

Nguyen, T; Li, GE; Chen, H; Cranfield, CG; McGrath, KC; Gorrie, CA

Neurological effects in the offspring after switching from tobacco cigarettes to e-cigarettes during pregnancy in a mouse model.

Nguyen, T

Li, GE Chen, H

Cranfield, CG McGrath, KC Gorrie, CA

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Publisher:: Oxford University Press (OUP)
Publication Type:: Journal Article
Citation:: Toxicological Sciences, 2019, 172, (1), pp. 191-200
Issue Date:: 2019-11

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Field	Value	Language
dc.contributor.author	Nguyen, T https://orcid.org/0000-0001-6371-4105
dc.contributor.author	Li, GE
dc.contributor.author	Chen, H https://orcid.org/0000-0001-6883-3752
dc.contributor.author	Cranfield, CG
dc.contributor.author	McGrath, KC
dc.contributor.author	Gorrie, CA
dc.date.accessioned	2020-05-03T21:23:33Z
dc.date.available	2019-08-17
dc.date.available	2020-05-03T21:23:33Z
dc.date.issued	2019-11
dc.identifier.citation	Toxicological Sciences, 2019, 172, (1), pp. 191-200
dc.identifier.issn	1096-0929
dc.identifier.issn	1096-0929
dc.identifier.uri	http://hdl.handle.net/10453/140423
dc.description.abstract	BACKGROUND:Maternal smoking is currently a public health concern and has been associated with a number of complications in the offspring. E-cigarettes are gaining popularity as a 'safer' alternative to tobacco cigarettes during pregnancy, however, there are a limited number of studies to suggest that it is actually 'safe'. STUDY DESIGN:Balb/C female mice were exposed to ambient air (n = 8; Sham), or tobacco cigarette smoke (n = 8; SE) before gestation, during gestation and lactation. A third group was exposed to cigarette smoke before gestation followed by e-cigarette aerosols during gestation and lactation (n = 8; Switch). Male offspring (12-week old, n = 10-14/group) underwent behavioural assessments to investigate short-term memory, anxiety and activity using the novel object recognition (NOR) and elevated plus maze (EPM) tests. Brains were collected at postnatal day (P)1, P20 and Week13 for global DNA methylation, epigenetic gene expression, and neuronal cell counts. RESULTS:The offspring from mothers switching to e-cigarettes exhibited no change in exploration/activity, but showed a decrease in global DNA methylation, Aurora Kinase (Aurk) A and AurkB gene expression and a reduction in neuronal cell numbers in the cornu ammonis 1 region of the dorsal hippocampus compared to the SE group. CONCLUSIONS:Continuous tobacco cigarette smoke exposure during pregnancy resulted in marked neurological deficits in the offspring. Switching to e-cigarettes during pregnancy reduced these neurological deficits compared to cigarette smoke exposure. However, neurological changes were still observed, so we therefore conclude that e-cigarette use during pregnancy is not advised.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Oxford University Press (OUP)
dc.relation.ispartof	Toxicological Sciences
dc.relation.isbasedon	10.1093/toxsci/kfz194
dc.rights	This is a pre-copyedited, author-produced version of an article accepted for publication in [Toxicological Sciences] following peer review. The version of record [Toxicological Sciences, 2019, 172, (1), pp. 191-200] is available online at: http://doi.org/10.1093/toxsci/kfz194
dc.rights	info:eu-repo/semantics/embargoedAccess
dc.subject	1115 Pharmacology and Pharmaceutical Sciences
dc.subject.classification	Toxicology
dc.title	Neurological effects in the offspring after switching from tobacco cigarettes to e-cigarettes during pregnancy in a mouse model.
dc.type	Journal Article
utslib.citation.volume	172
utslib.location.activity	United States
utslib.for	1115 Pharmacology and Pharmaceutical Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	open_access	*
pubs.consider-herdc	true
utslib.copyright.embargo	2021-08-28T00:00:00+1000Z
dc.date.updated	2020-05-03T21:23:30Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	172
utslib.start-page	191
utslib.citation.issue	1

Abstract:

BACKGROUND:Maternal smoking is currently a public health concern and has been associated with a number of complications in the offspring. E-cigarettes are gaining popularity as a 'safer' alternative to tobacco cigarettes during pregnancy, however, there are a limited number of studies to suggest that it is actually 'safe'. STUDY DESIGN:Balb/C female mice were exposed to ambient air (n = 8; Sham), or tobacco cigarette smoke (n = 8; SE) before gestation, during gestation and lactation. A third group was exposed to cigarette smoke before gestation followed by e-cigarette aerosols during gestation and lactation (n = 8; Switch). Male offspring (12-week old, n = 10-14/group) underwent behavioural assessments to investigate short-term memory, anxiety and activity using the novel object recognition (NOR) and elevated plus maze (EPM) tests. Brains were collected at postnatal day (P)1, P20 and Week13 for global DNA methylation, epigenetic gene expression, and neuronal cell counts. RESULTS:The offspring from mothers switching to e-cigarettes exhibited no change in exploration/activity, but showed a decrease in global DNA methylation, Aurora Kinase (Aurk) A and AurkB gene expression and a reduction in neuronal cell numbers in the cornu ammonis 1 region of the dorsal hippocampus compared to the SE group. CONCLUSIONS:Continuous tobacco cigarette smoke exposure during pregnancy resulted in marked neurological deficits in the offspring. Switching to e-cigarettes during pregnancy reduced these neurological deficits compared to cigarette smoke exposure. However, neurological changes were still observed, so we therefore conclude that e-cigarette use during pregnancy is not advised.

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http://hdl.handle.net/10453/140423