A guide to Open-JIP, a low-cost open-source chlorophyll fluorometer

Bates, H; Zavafer, A; Szabó, M; Ralph, PJ

A guide to Open-JIP, a low-cost open-source chlorophyll fluorometer

Bates, H Zavafer, A

Szabó, M

Ralph, PJ

Permalink

Publication Type:: Journal Article
Citation:: Photosynthesis Research, 2019, 142 (3), pp. 361 - 368
Issue Date:: 2019-12-01

Recently Added

	Filename	Size
	s11120-019-00673-2.pdf	2.73 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is new to OPUS and is not currently available.

Full metadata record

Field	Value	Language
dc.contributor.author	Bates, H	en_US
dc.contributor.author	Zavafer, A https://orcid.org/0000-0002-8905-1618	en_US
dc.contributor.author	Szabó, M https://orcid.org/0000-0001-6235-2894	en_US
dc.contributor.author	Ralph, PJ https://orcid.org/0000-0002-3103-7346	en_US
dc.date.accessioned	2020-06-11T06:50:44Z
dc.date.accessioned	2020-06-11T06:50:45Z
dc.date.available	2019-09-09	en_US
dc.date.available	2020-06-11T06:50:44Z
dc.date.available	2020-06-11T06:50:45Z
dc.date.issued	2019-12-01	en_US
dc.identifier.citation	Photosynthesis Research, 2019, 142 (3), pp. 361 - 368	en_US
dc.identifier.issn	0166-8595	en_US
dc.identifier.uri	http://hdl.handle.net/10453/141319
dc.description.abstract	© 2019, Springer Nature B.V. Chlorophyll a fluorescence is the most widely used method to study photosynthesis and plant stress. While several commercial fluorometers are available, there is a need for a low-cost and highly customisable chlorophyll fluorometer. Such a device would aid in performing high-throughput assessment of photosynthesis, as these instruments can be mass-produced. Novel investigations into photosynthesis can also be performed as a result of the user’s ability to modify the devices functionality for their specific needs. Motivated by this, we present an open-source chlorophyll fluorometer based on the Kautsky induction curve (OJIP). The instrument consists of low-cost, easy-to-acquire electrical components and an open-source microcontroller (Arduino Mega) whose performance is equivalent to that of commercial instruments. Two 3D printable Open-JIP configurations are presented, one for higher plants and the other for microalgae cells in suspension. Directions for its construction are presented and the instrument is benchmarked against widely used commercial chlorophyll fluorometers.	en_US
dc.relation.ispartof	Photosynthesis Research	en_US
dc.relation.isbasedon	10.1007/s11120-019-00673-2	en_US
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject.classification	Plant Biology & Botany	en_US
dc.title	A guide to Open-JIP, a low-cost open-source chlorophyll fluorometer	en_US
dc.type	Journal Article
utslib.citation.volume	3	en_US
utslib.citation.volume	142	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	0604 Genetics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	recently_added	*
pubs.issue	3	en_US
pubs.publication-status	Published	en_US
pubs.volume	142	en_US

Abstract:

© 2019, Springer Nature B.V. Chlorophyll a fluorescence is the most widely used method to study photosynthesis and plant stress. While several commercial fluorometers are available, there is a need for a low-cost and highly customisable chlorophyll fluorometer. Such a device would aid in performing high-throughput assessment of photosynthesis, as these instruments can be mass-produced. Novel investigations into photosynthesis can also be performed as a result of the user’s ability to modify the devices functionality for their specific needs. Motivated by this, we present an open-source chlorophyll fluorometer based on the Kautsky induction curve (OJIP). The instrument consists of low-cost, easy-to-acquire electrical components and an open-source microcontroller (Arduino Mega) whose performance is equivalent to that of commercial instruments. Two 3D printable Open-JIP configurations are presented, one for higher plants and the other for microalgae cells in suspension. Directions for its construction are presented and the instrument is benchmarked against widely used commercial chlorophyll fluorometers.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/141319