Analysis by proteomics reveals unique circulatory proteins in idiopathic pulmonary fibrosis

Moodley, YP; Corte, TJ; Oliver, BG; Glaspole, IN; Livk, A; Ito, J; Peters, K; Lipscombe, R; Casey, T; Tan, DBA

Analysis by proteomics reveals unique circulatory proteins in idiopathic pulmonary fibrosis

Moodley, YP Corte, TJ Oliver, BG

Glaspole, IN Livk, A Ito, J Peters, K Lipscombe, R Casey, T Tan, DBA

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Publication Type:: Journal Article
Citation:: Respirology, 2019, 24 (11), pp. 1111 - 1114
Issue Date:: 2019-11-01

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Field	Value	Language
dc.contributor.author	Moodley, YP	en_US
dc.contributor.author	Corte, TJ	en_US
dc.contributor.author	Oliver, BG https://orcid.org/0000-0002-7122-9262	en_US
dc.contributor.author	Glaspole, IN	en_US
dc.contributor.author	Livk, A	en_US
dc.contributor.author	Ito, J	en_US
dc.contributor.author	Peters, K	en_US
dc.contributor.author	Lipscombe, R	en_US
dc.contributor.author	Casey, T	en_US
dc.contributor.author	Tan, DBA	en_US
dc.date.accessioned	2020-06-11T06:50:52Z
dc.date.accessioned	2020-06-11T06:50:52Z
dc.date.available	2019-06-04	en_US
dc.date.available	2020-06-11T06:50:52Z
dc.date.available	2020-06-11T06:50:52Z
dc.date.issued	2019-11-01	en_US
dc.identifier.citation	Respirology, 2019, 24 (11), pp. 1111 - 1114	en_US
dc.identifier.issn	1323-7799	en_US
dc.identifier.uri	http://hdl.handle.net/10453/141325
dc.description.abstract	© 2019 Asian Pacific Society of Respirology Background and objective: Idiopathic pulmonary fibrosis (IPF) is a progressive fibrotic disease that has a poor 3-year median survival rate with unclear pathophysiology. Radiological features include bibasal, subpleural fibrosis and honeycombing while its pathology is characterized by fibroblastic foci and honeycombing. Proteomic analysis of circulating molecules in plasma may identify factors that characterize IPF and may assist in the diagnosis, prognostication and determination of pathogenic pathways in this condition. Methods: Two independent quantitative proteomic techniques were used, isobaric tags for relative and absolute quantitation (iTRAQ) and multiple reaction monitoring (MRM), to identify differentially expressed plasma proteins in a group of IPF patients in comparison to healthy controls with normal lung function matched for age and gender. Results: Five proteins were identified to be differentially expressed in IPF compared to healthy controls (upregulation of platelet basic protein and downregulation of actin, cytoplasmic 2, antithrombin-III, extracellular matrix protein-1 and fibronectin). Conclusion: This study further validates the combinational use of non-targeted discovery proteomics (iTRAQ) with targeted quantitation by mass spectrometry (MRM) of soluble biomarkers to identify potentially important molecules and pathways for pulmonary diseases such as IPF.	en_US
dc.relation.ispartof	Respirology	en_US
dc.relation.isbasedon	10.1111/resp.13668	en_US
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject.classification	Respiratory System	en_US
dc.title	Analysis by proteomics reveals unique circulatory proteins in idiopathic pulmonary fibrosis	en_US
dc.type	Journal Article
utslib.citation.volume	11	en_US
utslib.citation.volume	24	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	recently_added	*
pubs.issue	11	en_US
pubs.publication-status	Published	en_US
pubs.volume	24	en_US

Abstract:

© 2019 Asian Pacific Society of Respirology Background and objective: Idiopathic pulmonary fibrosis (IPF) is a progressive fibrotic disease that has a poor 3-year median survival rate with unclear pathophysiology. Radiological features include bibasal, subpleural fibrosis and honeycombing while its pathology is characterized by fibroblastic foci and honeycombing. Proteomic analysis of circulating molecules in plasma may identify factors that characterize IPF and may assist in the diagnosis, prognostication and determination of pathogenic pathways in this condition. Methods: Two independent quantitative proteomic techniques were used, isobaric tags for relative and absolute quantitation (iTRAQ) and multiple reaction monitoring (MRM), to identify differentially expressed plasma proteins in a group of IPF patients in comparison to healthy controls with normal lung function matched for age and gender. Results: Five proteins were identified to be differentially expressed in IPF compared to healthy controls (upregulation of platelet basic protein and downregulation of actin, cytoplasmic 2, antithrombin-III, extracellular matrix protein-1 and fibronectin). Conclusion: This study further validates the combinational use of non-targeted discovery proteomics (iTRAQ) with targeted quantitation by mass spectrometry (MRM) of soluble biomarkers to identify potentially important molecules and pathways for pulmonary diseases such as IPF.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/141325