Emerging technologies for profiling extracellular vesicle heterogeneity.
- Publisher:
- ROYAL SOC CHEMISTRY
- Publication Type:
- Journal Article
- Citation:
- Lab on a chip, 2020, 20, (14), pp. 2423-2437
- Issue Date:
- 2020-07
Closed Access
Filename | Description | Size | |||
---|---|---|---|---|---|
authorreprints.pdf | 6.64 MB |
Copyright Clearance Process
- Recently Added
- In Progress
- Closed Access
This item is closed access and not available.
Extracellular vesicles (EVs) are membrane-bound vesicles secreted by most cell types and exist in virtually all bodily fluids. They carry on a wealth of proteomic and genetic information including proteins, lipids, miRNAs, mRNA, non-coding RNA and other molecules from parental cells. Increasing evidence shows that within populations of EVs, their biogenesis, physical characteristics (e.g. size, density, morphology) and cargos (e.g. protein, lipid content, nucleic acids) may vary substantially, which accordingly change their biological properties. To fully exploit the potential of EVs, it requires qualified methods to profile EV heterogeneity. In this review, we survey recent approaches for EV isolation with innovative discoveries in heterogeneity. The main challenges in EV heterogeneity research are identified, and the roles of single cell EV profiling and single EV imaging are highlighted. We further discuss promising opportunities for resolving the underlying complexity of EV heterogeneity.
Please use this identifier to cite or link to this item: