Analysis of barotactic and chemotactic guidance cues on directional decision-making of Dictyostelium discoideum cells in confined environments.

Belotti, Y; McGloin, D; Weijer, CJ

Analysis of barotactic and chemotactic guidance cues on directional decision-making of Dictyostelium discoideum cells in confined environments.

Belotti, Y McGloin, D

Weijer, CJ

Permalink

Publisher:: NATL ACAD SCIENCES
Publication Type:: Journal Article
Citation:: Proceedings of the National Academy of Sciences of the United States of America, 2020, 117, (41), pp. 25553-25559
Issue Date:: 2020-10

Closed Access

	Filename	Description	Size
	25553.full.pdf	Published version	1.11 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Belotti, Y
dc.contributor.author	McGloin, D https://orcid.org/0000-0002-0075-4481
dc.contributor.author	Weijer, CJ
dc.date.accessioned	2020-11-21T05:57:07Z
dc.date.available	2020-11-21T05:57:07Z
dc.date.issued	2020-10
dc.identifier.citation	Proceedings of the National Academy of Sciences of the United States of America, 2020, 117, (41), pp. 25553-25559
dc.identifier.issn	0027-8424
dc.identifier.issn	1091-6490
dc.identifier.uri	http://hdl.handle.net/10453/144206
dc.description.abstract	Neutrophils and dendritic cells when migrating in confined environments have been shown to actuate a directional choice toward paths of least hydraulic resistance (barotaxis), in some cases overriding chemotactic responses. Here, we investigate whether this barotactic response is conserved in the more primitive model organism Dictyostelium discoideum using a microfluidic chip design. This design allowed us to monitor the behavior of single cells via live imaging when confronted with bifurcating microchannels, presenting different combinations of hydraulic and chemical stimuli. Under the conditions employed we find no evidence in support of a barotactic response; the cells base their directional choices on the chemotactic cues. When the cells are confronted by a microchannel bifurcation, they often split their leading edge and start moving into both channels, before a decision is made to move into one and retract from the other channel. Analysis of this decision-making process has shown that cells in steeper nonhydrolyzable adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer (cAMPS) gradients move faster and split more readily. Furthermore, there exists a highly significant strong correlation between the velocity of the pseudopod moving up the cAMPS gradient to the total velocity of the pseudopods moving up and down the gradient over a large range of velocities. This suggests a role for a critical cortical tension gradient in the directional decision-making process.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	NATL ACAD SCIENCES
dc.relation.ispartof	Proceedings of the National Academy of Sciences of the United States of America
dc.relation.isbasedon	10.1073/pnas.2000686117
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.title	Analysis of barotactic and chemotactic guidance cues on directional decision-making of Dictyostelium discoideum cells in confined environments.
dc.type	Journal Article
utslib.citation.volume	117
utslib.location.activity	United States
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2020-11-21T05:57:03Z
pubs.issue	41
pubs.publication-status	Published
pubs.volume	117
utslib.citation.issue	41

Abstract:

Neutrophils and dendritic cells when migrating in confined environments have been shown to actuate a directional choice toward paths of least hydraulic resistance (barotaxis), in some cases overriding chemotactic responses. Here, we investigate whether this barotactic response is conserved in the more primitive model organism Dictyostelium discoideum using a microfluidic chip design. This design allowed us to monitor the behavior of single cells via live imaging when confronted with bifurcating microchannels, presenting different combinations of hydraulic and chemical stimuli. Under the conditions employed we find no evidence in support of a barotactic response; the cells base their directional choices on the chemotactic cues. When the cells are confronted by a microchannel bifurcation, they often split their leading edge and start moving into both channels, before a decision is made to move into one and retract from the other channel. Analysis of this decision-making process has shown that cells in steeper nonhydrolyzable adenosine- 3', 5'- cyclic monophosphorothioate, Sp- isomer (cAMPS) gradients move faster and split more readily. Furthermore, there exists a highly significant strong correlation between the velocity of the pseudopod moving up the cAMPS gradient to the total velocity of the pseudopods moving up and down the gradient over a large range of velocities. This suggests a role for a critical cortical tension gradient in the directional decision-making process.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/144206