Mechanical control of mitotic progression in single animal cells.
- Publisher:
- NATL ACAD SCIENCES
- Publication Type:
- Journal Article
- Citation:
- Proceedings of the National Academy of Sciences of the United States of America, 2015, 112, (36), pp. 11258-11263
- Issue Date:
- 2015-09
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Cattin, CJ | |
dc.contributor.author | Düggelin, M | |
dc.contributor.author | Martinez-Martin, D | |
dc.contributor.author | Gerber, C | |
dc.contributor.author | Müller, DJ | |
dc.contributor.author | Stewart, MP | |
dc.date.accessioned | 2020-11-25T07:44:55Z | |
dc.date.available | 2020-11-25T07:44:55Z | |
dc.date.issued | 2015-09 | |
dc.identifier.citation | Proceedings of the National Academy of Sciences of the United States of America, 2015, 112, (36), pp. 11258-11263 | |
dc.identifier.issn | 0027-8424 | |
dc.identifier.issn | 1091-6490 | |
dc.identifier.uri | http://hdl.handle.net/10453/144346 | |
dc.description.abstract | Despite the importance of mitotic cell rounding in tissue development and cell proliferation, there remains a paucity of approaches to investigate the mechanical robustness of cell rounding. Here we introduce ion beam-sculpted microcantilevers that enable precise force-feedback-controlled confinement of single cells while characterizing their progression through mitosis. We identify three force regimes according to the cell response: small forces (∼5 nN) that accelerate mitotic progression, intermediate forces where cells resist confinement (50-100 nN), and yield forces (>100 nN) where a significant decline in cell height impinges on microtubule spindle function, thereby inhibiting mitotic progression. Yield forces are coincident with a nonlinear drop in cell height potentiated by persistent blebbing and loss of cortical F-actin homogeneity. Our results suggest that a buildup of actomyosin-dependent cortical tension and intracellular pressure precedes mechanical failure, or herniation, of the cell cortex at the yield force. Thus, we reveal how the mechanical properties of mitotic cells and their response to external forces are linked to mitotic progression under conditions of mechanical confinement. | |
dc.format | Print-Electronic | |
dc.language | eng | |
dc.publisher | NATL ACAD SCIENCES | |
dc.relation.ispartof | Proceedings of the National Academy of Sciences of the United States of America | |
dc.relation.isbasedon | 10.1073/pnas.1502029112 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject.mesh | Hela Cells | |
dc.subject.mesh | Microtubules | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Actomyosin | |
dc.subject.mesh | Myosin Heavy Chains | |
dc.subject.mesh | Luminescent Proteins | |
dc.subject.mesh | Histones | |
dc.subject.mesh | Microscopy, Electron, Scanning | |
dc.subject.mesh | Microscopy, Atomic Force | |
dc.subject.mesh | Reproducibility of Results | |
dc.subject.mesh | Mitosis | |
dc.subject.mesh | Cell Shape | |
dc.subject.mesh | Molecular Motor Proteins | |
dc.subject.mesh | Single-Cell Analysis | |
dc.subject.mesh | Spindle Apparatus | |
dc.subject.mesh | Actomyosin | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Cell Shape | |
dc.subject.mesh | HeLa Cells | |
dc.subject.mesh | Histones | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Luminescent Proteins | |
dc.subject.mesh | Microscopy, Atomic Force | |
dc.subject.mesh | Microscopy, Electron, Scanning | |
dc.subject.mesh | Microtubules | |
dc.subject.mesh | Mitosis | |
dc.subject.mesh | Molecular Motor Proteins | |
dc.subject.mesh | Myosin Heavy Chains | |
dc.subject.mesh | Reproducibility of Results | |
dc.subject.mesh | Single-Cell Analysis | |
dc.subject.mesh | Spindle Apparatus | |
dc.title | Mechanical control of mitotic progression in single animal cells. | |
dc.type | Journal Article | |
utslib.citation.volume | 112 | |
utslib.location.activity | United States | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | /University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science/School of Life Sciences | |
pubs.organisational-group | /University of Technology Sydney | |
utslib.copyright.status | open_access | * |
dc.date.updated | 2020-11-25T07:44:51Z | |
pubs.issue | 36 | |
pubs.publication-status | Published | |
pubs.volume | 112 | |
utslib.citation.issue | 36 |
Abstract:
Despite the importance of mitotic cell rounding in tissue development and cell proliferation, there remains a paucity of approaches to investigate the mechanical robustness of cell rounding. Here we introduce ion beam-sculpted microcantilevers that enable precise force-feedback-controlled confinement of single cells while characterizing their progression through mitosis. We identify three force regimes according to the cell response: small forces (∼5 nN) that accelerate mitotic progression, intermediate forces where cells resist confinement (50-100 nN), and yield forces (>100 nN) where a significant decline in cell height impinges on microtubule spindle function, thereby inhibiting mitotic progression. Yield forces are coincident with a nonlinear drop in cell height potentiated by persistent blebbing and loss of cortical F-actin homogeneity. Our results suggest that a buildup of actomyosin-dependent cortical tension and intracellular pressure precedes mechanical failure, or herniation, of the cell cortex at the yield force. Thus, we reveal how the mechanical properties of mitotic cells and their response to external forces are linked to mitotic progression under conditions of mechanical confinement.
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