Mitochondrial dynamics quantitatively revealed by STED nanoscopy with an enhanced squaraine variant probe.
- Publisher:
- NATURE PUBLISHING GROUP
- Publication Type:
- Journal Article
- Citation:
- Nature communications, 2020, 11, (1)
- Issue Date:
- 2020-07-24
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Yang, X | |
dc.contributor.author | Yang, Z | |
dc.contributor.author | Wu, Z | |
dc.contributor.author | He, Y | |
dc.contributor.author | Shan, C | |
dc.contributor.author | Chai, P | |
dc.contributor.author | Ma, C | |
dc.contributor.author | Tian, M | |
dc.contributor.author | Teng, J | |
dc.contributor.author |
Jin, D https://orcid.org/0000-0003-1046-2666 |
|
dc.contributor.author | Yan, W | |
dc.contributor.author | Das, P | |
dc.contributor.author | Qu, J | |
dc.contributor.author | Xi, P | |
dc.date.accessioned | 2021-01-07T22:09:03Z | |
dc.date.available | 2020-07-03 | |
dc.date.available | 2021-01-07T22:09:03Z | |
dc.date.issued | 2020-07-24 | |
dc.identifier.citation | Nature communications, 2020, 11, (1) | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | http://hdl.handle.net/10453/145195 | |
dc.description.abstract | Mitochondria play a critical role in generating energy to support the entire lifecycle of biological cells, yet it is still unclear how their morphological structures evolve to regulate their functionality. Conventional fluorescence microscopy can only provide ~300 nm resolution, which is insufficient to visualize mitochondrial cristae. Here, we developed an enhanced squaraine variant dye (MitoESq-635) to study the dynamic structures of mitochondrial cristae in live cells with a superresolution technique. The low saturation intensity and high photostability of MitoESq-635 make it ideal for long-term, high-resolution (stimulated emission depletion) STED nanoscopy. We performed time-lapse imaging of the mitochondrial inner membrane over 50 min (3.9 s per frame, with 71.5 s dark recovery) in living HeLa cells with a resolution of 35.2 nm. The forms of the cristae during mitochondrial fusion and fission can be clearly observed. Our study demonstrates the emerging capability of optical STED nanoscopy to investigate intracellular physiological processes with nanoscale resolution for an extended period of time. | |
dc.format | Electronic | |
dc.language | eng | |
dc.publisher | NATURE PUBLISHING GROUP | |
dc.relation.ispartof | Nature communications | |
dc.relation.isbasedon | 10.1038/s41467-020-17546-1 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject.mesh | Cell Line | |
dc.subject.mesh | Hela Cells | |
dc.subject.mesh | Mitochondria | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Cyclobutanes | |
dc.subject.mesh | Phenols | |
dc.subject.mesh | Fluorescent Dyes | |
dc.subject.mesh | Microscopy, Fluorescence | |
dc.subject.mesh | Staining and Labeling | |
dc.subject.mesh | Nanotechnology | |
dc.subject.mesh | Mitochondrial Membranes | |
dc.subject.mesh | Mitochondrial Dynamics | |
dc.subject.mesh | Cell Line | |
dc.subject.mesh | Hela Cells | |
dc.subject.mesh | Mitochondria | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Cyclobutanes | |
dc.subject.mesh | Phenols | |
dc.subject.mesh | Fluorescent Dyes | |
dc.subject.mesh | Microscopy, Fluorescence | |
dc.subject.mesh | Staining and Labeling | |
dc.subject.mesh | Nanotechnology | |
dc.subject.mesh | Mitochondrial Membranes | |
dc.subject.mesh | Mitochondrial Dynamics | |
dc.subject.mesh | Cell Line | |
dc.subject.mesh | Cyclobutanes | |
dc.subject.mesh | Fluorescent Dyes | |
dc.subject.mesh | HeLa Cells | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Microscopy, Fluorescence | |
dc.subject.mesh | Mitochondria | |
dc.subject.mesh | Mitochondrial Dynamics | |
dc.subject.mesh | Mitochondrial Membranes | |
dc.subject.mesh | Nanotechnology | |
dc.subject.mesh | Phenols | |
dc.subject.mesh | Staining and Labeling | |
dc.title | Mitochondrial dynamics quantitatively revealed by STED nanoscopy with an enhanced squaraine variant probe. | |
dc.type | Journal Article | |
utslib.citation.volume | 11 | |
utslib.location.activity | England | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | /University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences | |
pubs.organisational-group | /University of Technology Sydney | |
utslib.copyright.status | open_access | * |
pubs.consider-herdc | false | |
dc.date.updated | 2021-01-07T22:08:42Z | |
pubs.issue | 1 | |
pubs.publication-status | Published | |
pubs.volume | 11 | |
utslib.citation.issue | 1 |
Abstract:
Mitochondria play a critical role in generating energy to support the entire lifecycle of biological cells, yet it is still unclear how their morphological structures evolve to regulate their functionality. Conventional fluorescence microscopy can only provide ~300 nm resolution, which is insufficient to visualize mitochondrial cristae. Here, we developed an enhanced squaraine variant dye (MitoESq-635) to study the dynamic structures of mitochondrial cristae in live cells with a superresolution technique. The low saturation intensity and high photostability of MitoESq-635 make it ideal for long-term, high-resolution (stimulated emission depletion) STED nanoscopy. We performed time-lapse imaging of the mitochondrial inner membrane over 50 min (3.9 s per frame, with 71.5 s dark recovery) in living HeLa cells with a resolution of 35.2 nm. The forms of the cristae during mitochondrial fusion and fission can be clearly observed. Our study demonstrates the emerging capability of optical STED nanoscopy to investigate intracellular physiological processes with nanoscale resolution for an extended period of time.
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