MKPV (aka MuCPV) and related chapparvoviruses are nephro-tropic and encode novel accessory proteins p15 and NS2

Jolly, C; Lee, Q; Padula, M; Pinello, N; Williams, S; O’Rourke, M; Fumagalli, MJ; Orkin, J; Shaban, B; Brenner, O; Weninger, W; de Souza, WM; Melin, A; Wong, JJ-L; Crim, M; Monette, S; Roediger, B

MKPV (aka MuCPV) and related chapparvoviruses are nephro-tropic and encode novel accessory proteins p15 and NS2

Jolly, C Lee, Q Padula, M

Pinello, N Williams, S O’Rourke, M Fumagalli, MJ Orkin, J Shaban, B Brenner, O Weninger, W de Souza, WM Melin, A Wong, JJ-L Crim, M Monette, S Roediger, B

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Publisher:: Cold Spring Harbor Laboratory
Publication Type:: Journal Article
Citation:: 2020
Issue Date:: 2020

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Field	Value	Language
dc.contributor.author	Jolly, C
dc.contributor.author	Lee, Q
dc.contributor.author	Padula, M https://orcid.org/0000-0002-8283-0643
dc.contributor.author	Pinello, N
dc.contributor.author	Williams, S
dc.contributor.author	O’Rourke, M
dc.contributor.author	Fumagalli, MJ
dc.contributor.author	Orkin, J
dc.contributor.author	Shaban, B
dc.contributor.author	Brenner, O
dc.contributor.author	Weninger, W
dc.contributor.author	de Souza, WM
dc.contributor.author	Melin, A
dc.contributor.author	Wong, JJ-L
dc.contributor.author	Crim, M
dc.contributor.author	Monette, S
dc.contributor.author	Roediger, B
dc.date.accessioned	2021-01-07T22:12:10Z
dc.date.available	2021-01-07T22:12:10Z
dc.date.issued	2020
dc.identifier.citation	2020
dc.identifier.uri	http://hdl.handle.net/10453/145196
dc.description.abstract	Abstract Mouse kidney parvovirus (MKPV) is a member of the provisional Chapparvovirus genus that causes renal disease in immune-compromised mice, with a disease course reminiscent of polyomavirus-associated nephropathy in immune-suppressed kidney transplant patients. Here we map four MKPV transcripts, created by alternative splicing, to a common transcription initiation region, and use mass spectrometry to identify “p10” and “p15” as novel chapparvovirus accessory proteins produced in MKPV-infected kidneys. p15 and a splicing-dependent putative accessory protein NS2 are conserved in all near-complete tetrapod chapparvovirus genomes currently available (from mammals, birds and a reptile). In contrast, p10 may be encoded only by viruses with >60% amino acid identity to MKPV. We show that MKPV is kidney-tropic and that the bat chapparvovirus DrPV-1 and a non-human primate chapparvovirus, CKPV, are also found in the kidneys of their hosts. We propose, therefore, that chapparvoviruses with >60% VP1 amino acid identity to MKPV be classified into a genus dubbed Nephroparvovirus , which is consistent with nomenclature for the genus Erythroparvovirus .
dc.language	en
dc.publisher	Cold Spring Harbor Laboratory
dc.relation.isbasedon	10.1101/732537
dc.rights	info:eu-repo/semantics/openAccess
dc.title	MKPV (aka MuCPV) and related chapparvoviruses are nephro-tropic and encode novel accessory proteins p15 and NS2
dc.type	Journal Article
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	open_access	*
pubs.consider-herdc	true
dc.date.updated	2021-01-07T22:11:52Z
pubs.publication-status	In preparation

Abstract:

Abstract Mouse kidney parvovirus (MKPV) is a member of the provisional Chapparvovirus genus that causes renal disease in immune-compromised mice, with a disease course reminiscent of polyomavirus-associated nephropathy in immune-suppressed kidney transplant patients. Here we map four MKPV transcripts, created by alternative splicing, to a common transcription initiation region, and use mass spectrometry to identify “p10” and “p15” as novel chapparvovirus accessory proteins produced in MKPV-infected kidneys. p15 and a splicing-dependent putative accessory protein NS2 are conserved in all near-complete tetrapod chapparvovirus genomes currently available (from mammals, birds and a reptile). In contrast, p10 may be encoded only by viruses with >60% amino acid identity to MKPV. We show that MKPV is kidney-tropic and that the bat chapparvovirus DrPV-1 and a non-human primate chapparvovirus, CKPV, are also found in the kidneys of their hosts. We propose, therefore, that chapparvoviruses with >60% VP1 amino acid identity to MKPV be classified into a genus dubbed Nephroparvovirus , which is consistent with nomenclature for the genus Erythroparvovirus .

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http://hdl.handle.net/10453/145196