Nanoparticle Tracking Analysis-Based in Vitro Detection of Critical Biomarkers

Zhang, W; Liang, J; Lu, X; Ren, W; Liu, C

Nanoparticle Tracking Analysis-Based in Vitro Detection of Critical Biomarkers

Zhang, W Liang, J Lu, X Ren, W

Liu, C

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Publisher:: AMER CHEMICAL SOC
Publication Type:: Journal Article
Citation:: ACS Applied Nano Materials, 2020, 3, (3), pp. 2881-2888
Issue Date:: 2020-01-01

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Field	Value	Language
dc.contributor.author	Zhang, W
dc.contributor.author	Liang, J
dc.contributor.author	Lu, X
dc.contributor.author	Ren, W https://orcid.org/0000-0002-6537-6039
dc.contributor.author	Liu, C
dc.date.accessioned	2021-01-08T02:28:11Z
dc.date.available	2021-01-08T02:28:11Z
dc.date.issued	2020-01-01
dc.identifier.citation	ACS Applied Nano Materials, 2020, 3, (3), pp. 2881-2888
dc.identifier.issn	2574-0970
dc.identifier.issn	2574-0970
dc.identifier.uri	http://hdl.handle.net/10453/145212
dc.description.abstract	© 2020 American Chemical Society. Nanoparticles emerge to be powerful probes for the sensing of critical biomarkers because of their characteristic optical/electrochemical/magnetic properties. However, the versatility and prevalence of nanoparticle-based assays remain hampered because each kind of nanoprobe has designated properties, which requires different instruments for the readout of the signals. Herein, a versatile nanoparticle tracking analysis (NTA)-based strategy is innovatively proposed for the in vitro detection of critical biomarkers without demanding specific properties of the nanoparticles. Based on the sandwich-type immunoreaction and the duplex-specific nuclease-assisted cycling nucleic acid cleavage, the quantitative relationships are rationally built up between the level of both protein and microRNA biomarkers and the nanoparticles' number variation. Under proper conditions, NTA will provide the absolute number count and accurate size distribution of the nanoparticles in the solution phase in a real-time manner to facilely determine the level of the protein and microRNA targets. Benefiting from the intrinsic ability of NTA for grouping the nanoparticles by size and composition, the multiplexed assay of biomolecules is also achieved. Compared with the conventional nanoparticle-based biosensing systems, NTA can directly count the nanoparticles in aqueous solution, without depending on the nanoparticles' specific properties. This work remarkably improves the versatility and convenience of nanoparticle counting-based bioanalysis, inspiring the future application of NTA in biosensing.
dc.language	English
dc.publisher	AMER CHEMICAL SOC
dc.relation.ispartof	ACS Applied Nano Materials
dc.relation.isbasedon	10.1021/acsanm.0c00154
dc.rights	info:eu-repo/semantics/closedAccess
dc.title	Nanoparticle Tracking Analysis-Based in Vitro Detection of Critical Biomarkers
dc.type	Journal Article
utslib.citation.volume	3
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2021-01-08T02:27:51Z
pubs.issue	3
pubs.publication-status	Published
pubs.volume	3
utslib.citation.issue	3

Abstract:

© 2020 American Chemical Society. Nanoparticles emerge to be powerful probes for the sensing of critical biomarkers because of their characteristic optical/electrochemical/magnetic properties. However, the versatility and prevalence of nanoparticle-based assays remain hampered because each kind of nanoprobe has designated properties, which requires different instruments for the readout of the signals. Herein, a versatile nanoparticle tracking analysis (NTA)-based strategy is innovatively proposed for the in vitro detection of critical biomarkers without demanding specific properties of the nanoparticles. Based on the sandwich-type immunoreaction and the duplex-specific nuclease-assisted cycling nucleic acid cleavage, the quantitative relationships are rationally built up between the level of both protein and microRNA biomarkers and the nanoparticles' number variation. Under proper conditions, NTA will provide the absolute number count and accurate size distribution of the nanoparticles in the solution phase in a real-time manner to facilely determine the level of the protein and microRNA targets. Benefiting from the intrinsic ability of NTA for grouping the nanoparticles by size and composition, the multiplexed assay of biomolecules is also achieved. Compared with the conventional nanoparticle-based biosensing systems, NTA can directly count the nanoparticles in aqueous solution, without depending on the nanoparticles' specific properties. This work remarkably improves the versatility and convenience of nanoparticle counting-based bioanalysis, inspiring the future application of NTA in biosensing.

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http://hdl.handle.net/10453/145212