Misincorporation Proteomics Technologies: A Review.

Steele, JR; Italiano, CJ; Phillips, CR; Violi, JP; Pu, L; Rodgers, KJ; Padula, MP

Misincorporation Proteomics Technologies: A Review.

Steele, JR Italiano, CJ Phillips, CR Violi, JP Pu, L Rodgers, KJ Padula, MP

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Publisher:: MDPI AG
Publication Type:: Journal Article
Citation:: Proteomes, 2021, 9, (1), pp. 1-20
Issue Date:: 2021-01-21

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Field	Value	Language
dc.contributor.author	Steele, JR
dc.contributor.author	Italiano, CJ
dc.contributor.author	Phillips, CR
dc.contributor.author	Violi, JP
dc.contributor.author	Pu, L
dc.contributor.author	Rodgers, KJ
dc.contributor.author	Padula, MP
dc.date.accessioned	2021-03-01T03:10:45Z
dc.date.available	2021-01-18
dc.date.available	2021-03-01T03:10:45Z
dc.date.issued	2021-01-21
dc.identifier.citation	Proteomes, 2021, 9, (1), pp. 1-20
dc.identifier.issn	2227-7382
dc.identifier.issn	2227-7382
dc.identifier.uri	http://hdl.handle.net/10453/146553
dc.description.abstract	Proteinopathies are diseases caused by factors that affect proteoform conformation. As such, a prevalent hypothesis is that the misincorporation of noncanonical amino acids into a proteoform results in detrimental structures. However, this hypothesis is missing proteomic evidence, specifically the detection of a noncanonical amino acid in a peptide sequence. This review aims to outline the current state of technology that can be used to investigate mistranslations and misincorporations whilst framing the pursuit as Misincorporation Proteomics (MiP). The current availability of technologies explored herein is mass spectrometry, sample enrichment/preparation, data analysis techniques, and the hyphenation of approaches. While many of these technologies show potential, our review reveals a need for further development and refinement of approaches is still required.
dc.format	Electronic
dc.language	eng
dc.publisher	MDPI AG
dc.relation.ispartof	Proteomes
dc.relation.isbasedon	10.3390/proteomes9010002
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0601 Biochemistry and Cell Biology
dc.title	Misincorporation Proteomics Technologies: A Review.
dc.type	Journal Article
utslib.citation.volume	9
utslib.location.activity	Switzerland
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney/Provost
pubs.organisational-group	/University of Technology Sydney/Provost/Jumbunna
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access	*
dc.date.updated	2021-03-01T03:10:37Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	9
utslib.citation.issue	1

Abstract:

Proteinopathies are diseases caused by factors that affect proteoform conformation. As such, a prevalent hypothesis is that the misincorporation of noncanonical amino acids into a proteoform results in detrimental structures. However, this hypothesis is missing proteomic evidence, specifically the detection of a noncanonical amino acid in a peptide sequence. This review aims to outline the current state of technology that can be used to investigate mistranslations and misincorporations whilst framing the pursuit as Misincorporation Proteomics (MiP). The current availability of technologies explored herein is mass spectrometry, sample enrichment/preparation, data analysis techniques, and the hyphenation of approaches. While many of these technologies show potential, our review reveals a need for further development and refinement of approaches is still required.

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http://hdl.handle.net/10453/146553