Fast field-cycling magnetic resonance detection of intracellular ultra-small iron oxide particles in vitro: Proof-of-concept.

Abbas, H; Broche, LM; Ezdoglian, A; Li, D; Yuecel, R; James Ross, P; Cheyne, L; Wilson, HM; Lurie, DJ; Dawson, DK

Fast field-cycling magnetic resonance detection of intracellular ultra-small iron oxide particles in vitro: Proof-of-concept.

Abbas, H Broche, LM Ezdoglian, A Li, D

Yuecel, R James Ross, P Cheyne, L Wilson, HM Lurie, DJ Dawson, DK

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Publisher:: ACADEMIC PRESS INC ELSEVIER SCIENCE
Publication Type:: Journal Article
Citation:: Journal of magnetic resonance (San Diego, Calif. : 1997), 2020, 313, pp. 106722
Issue Date:: 2020-04

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Field	Value	Language
dc.contributor.author	Abbas, H
dc.contributor.author	Broche, LM
dc.contributor.author	Ezdoglian, A
dc.contributor.author	Li, D https://orcid.org/0000-0002-7535-8184
dc.contributor.author	Yuecel, R
dc.contributor.author	James Ross, P
dc.contributor.author	Cheyne, L
dc.contributor.author	Wilson, HM
dc.contributor.author	Lurie, DJ
dc.contributor.author	Dawson, DK
dc.date.accessioned	2021-03-31T09:10:15Z
dc.date.available	2020-03-20
dc.date.available	2021-03-31T09:10:15Z
dc.date.issued	2020-04
dc.identifier.citation	Journal of magnetic resonance (San Diego, Calif. : 1997), 2020, 313, pp. 106722
dc.identifier.issn	1090-7807
dc.identifier.issn	1096-0856
dc.identifier.uri	http://hdl.handle.net/10453/147731
dc.description.abstract	<h4>Purpose</h4>Inflammation is central in disease pathophysiology and accurate methods for its detection and quantification are increasingly required to guide diagnosis and therapy. Here we explored the ability of Fast Field-Cycling Magnetic Resonance (FFC-MR) in quantifying the signal of ultra-small superparamagnetic iron oxide particles (USPIO) phagocytosed by J774 macrophage-like cells as a proof-of-principle.<h4>Methods</h4>Relaxation rates were measured in suspensions of J774 macrophage-like cells loaded with USPIO (0-200 μg/ml Fe as ferumoxytol), using a 0.25 T FFC benchtop relaxometer and a human whole-body, in-house built 0.2 T FFC-MR prototype system with a custom test tube coil. Identical non-imaging, saturation recovery pulse sequence with 90° flip angle and 20 different evolution fields selected logarithmically between 80 μT and 0.2 T (3.4 kHz and 8.51 MHz proton Larmor frequency [PLF] respectively). Results were compared with imaging flow cytometry quantification of side scatter intensity and USPIO-occupied cell area. A reference colorimetric iron assay was used.<h4>Results</h4>The T<sub>1</sub> dispersion curves derived from FFC-MR were excellent in detecting USPIO at all concentrations examined (0-200 μg/ml Fe as ferumoxytol) vs. control cells, p ≤ 0.001. FFC-NMR was capable of reliably detecting cellular iron content as low as 1.12 ng/µg cell protein, validated using a colorimetric assay. FFC-MR was comparable to imaging flow cytometry quantification of side scatter intensity but superior to USPIO-occupied cell area, the latter being only sensitive at exposures ≥ 10 µg/ml USPIO.<h4>Conclusions</h4>We demonstrated for the first time that FFC-MR is capable of quantitative assessment of intra-cellular iron which will have important implications for the use of USPIO in a variety of biological applications, including the study of inflammation.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ACADEMIC PRESS INC ELSEVIER SCIENCE
dc.relation.ispartof	Journal of magnetic resonance (San Diego, Calif. : 1997)
dc.relation.isbasedon	10.1016/j.jmr.2020.106722
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	02 Physical Sciences, 09 Engineering
dc.subject.classification	Biophysics
dc.title	Fast field-cycling magnetic resonance detection of intracellular ultra-small iron oxide particles in vitro: Proof-of-concept.
dc.type	Journal Article
utslib.citation.volume	313
utslib.location.activity	United States
utslib.for	029903 Medical Physics
utslib.for	0903 Biomedical Engineering
utslib.for	02 Physical Sciences
utslib.for	09 Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2021-03-31T09:10:11Z
pubs.publication-status	Published
pubs.volume	313

Abstract:

Purpose

Inflammation is central in disease pathophysiology and accurate methods for its detection and quantification are increasingly required to guide diagnosis and therapy. Here we explored the ability of Fast Field-Cycling Magnetic Resonance (FFC-MR) in quantifying the signal of ultra-small superparamagnetic iron oxide particles (USPIO) phagocytosed by J774 macrophage-like cells as a proof-of-principle.

Methods

Relaxation rates were measured in suspensions of J774 macrophage-like cells loaded with USPIO (0-200 μg/ml Fe as ferumoxytol), using a 0.25 T FFC benchtop relaxometer and a human whole-body, in-house built 0.2 T FFC-MR prototype system with a custom test tube coil. Identical non-imaging, saturation recovery pulse sequence with 90° flip angle and 20 different evolution fields selected logarithmically between 80 μT and 0.2 T (3.4 kHz and 8.51 MHz proton Larmor frequency [PLF] respectively). Results were compared with imaging flow cytometry quantification of side scatter intensity and USPIO-occupied cell area. A reference colorimetric iron assay was used.

Results

The T₁ dispersion curves derived from FFC-MR were excellent in detecting USPIO at all concentrations examined (0-200 μg/ml Fe as ferumoxytol) vs. control cells, p ≤ 0.001. FFC-NMR was capable of reliably detecting cellular iron content as low as 1.12 ng/µg cell protein, validated using a colorimetric assay. FFC-MR was comparable to imaging flow cytometry quantification of side scatter intensity but superior to USPIO-occupied cell area, the latter being only sensitive at exposures ≥ 10 µg/ml USPIO.

Conclusions

We demonstrated for the first time that FFC-MR is capable of quantitative assessment of intra-cellular iron which will have important implications for the use of USPIO in a variety of biological applications, including the study of inflammation.

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http://hdl.handle.net/10453/147731