Cell shape-independent FtsZ dynamics in synthetically remodeled bacterial cells.

Söderström, B; Badrutdinov, A; Chan, H; Skoglund, U

Cell shape-independent FtsZ dynamics in synthetically remodeled bacterial cells.

Söderström, B Badrutdinov, A Chan, H

Skoglund, U

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Publisher:: NATURE PUBLISHING GROUP
Publication Type:: Journal Article
Citation:: Nature communications, 2018, 9, (1), pp. 4323
Issue Date:: 2018-10-18

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Field	Value	Language
dc.contributor.author	Söderström, B
dc.contributor.author	Badrutdinov, A
dc.contributor.author	Chan, H https://orcid.org/0000-0002-5194-0006
dc.contributor.author	Skoglund, U
dc.date.accessioned	2021-04-02T11:20:14Z
dc.date.available	2018-10-03
dc.date.available	2021-04-02T11:20:14Z
dc.date.issued	2018-10-18
dc.identifier.citation	Nature communications, 2018, 9, (1), pp. 4323
dc.identifier.issn	2041-1723
dc.identifier.issn	2041-1723
dc.identifier.uri	http://hdl.handle.net/10453/147813
dc.description.abstract	FtsZ is the main regulator of bacterial cell division. It has been implicated in acting as a scaffolding protein for other division proteins, a force generator during constriction, and more recently, as an active regulator of septal cell wall production. FtsZ assembles into a heterogeneous structure coined the Z-ring due to its resemblance to a ring confined by the midcell geometry. Here, to establish a framework for examining geometrical influences on proper Z-ring assembly and dynamics, we sculpted Escherichia coli cells into unnatural shapes using division- and cell wall-specific inhibitors in a micro-fabrication scheme. This approach allowed us to examine FtsZ behavior in engineered Z-squares and Z-hearts. We use stimulated emission depletion (STED) nanoscopy to show that FtsZ clusters in sculpted cells maintain the same dimensions as their wild-type counterparts. Based on our results, we propose that the underlying membrane geometry is not a deciding factor for FtsZ cluster maintenance and dynamics in vivo.
dc.format	Electronic
dc.language	eng
dc.publisher	NATURE PUBLISHING GROUP
dc.relation.ispartof	Nature communications
dc.relation.isbasedon	10.1038/s41467-018-06887-7
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Bacterial Proteins
dc.subject.mesh	Cytoskeletal Proteins
dc.subject.mesh	Fluorescence
dc.subject.mesh	Artificial Cells
dc.subject.mesh	Artificial Cells
dc.subject.mesh	Bacterial Proteins
dc.subject.mesh	Cytoskeletal Proteins
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Fluorescence
dc.title	Cell shape-independent FtsZ dynamics in synthetically remodeled bacterial cells.
dc.type	Journal Article
utslib.citation.volume	9
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access	*
dc.date.updated	2021-04-02T11:20:09Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	9
utslib.citation.issue	1

Abstract:

FtsZ is the main regulator of bacterial cell division. It has been implicated in acting as a scaffolding protein for other division proteins, a force generator during constriction, and more recently, as an active regulator of septal cell wall production. FtsZ assembles into a heterogeneous structure coined the Z-ring due to its resemblance to a ring confined by the midcell geometry. Here, to establish a framework for examining geometrical influences on proper Z-ring assembly and dynamics, we sculpted Escherichia coli cells into unnatural shapes using division- and cell wall-specific inhibitors in a micro-fabrication scheme. This approach allowed us to examine FtsZ behavior in engineered Z-squares and Z-hearts. We use stimulated emission depletion (STED) nanoscopy to show that FtsZ clusters in sculpted cells maintain the same dimensions as their wild-type counterparts. Based on our results, we propose that the underlying membrane geometry is not a deciding factor for FtsZ cluster maintenance and dynamics in vivo.

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http://hdl.handle.net/10453/147813