Regional iron distribution and soluble ferroprotein profiles in the healthy human brain.
- Publisher:
- PERGAMON-ELSEVIER SCIENCE LTD
- Publication Type:
- Journal Article
- Citation:
- Progress in neurobiology, 2020, 186, pp. 101744
- Issue Date:
- 2020-03
Closed Access
Filename | Description | Size | |||
---|---|---|---|---|---|
1-s2.0-S0301008219303624-main.pdf | Published version | 1.57 MB | Adobe PDF |
Copyright Clearance Process
- Recently Added
- In Progress
- Closed Access
This item is closed access and not available.
Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | McAllum, EJ | |
dc.contributor.author | Hare, DJ | |
dc.contributor.author | Volitakis, I | |
dc.contributor.author | McLean, CA | |
dc.contributor.author | Bush, AI | |
dc.contributor.author | Finkelstein, DI | |
dc.contributor.author | Roberts, BR | |
dc.date.accessioned | 2021-04-03T03:19:59Z | |
dc.date.available | 2019-12-18 | |
dc.date.available | 2021-04-03T03:19:59Z | |
dc.date.issued | 2020-03 | |
dc.identifier.citation | Progress in neurobiology, 2020, 186, pp. 101744 | |
dc.identifier.issn | 0301-0082 | |
dc.identifier.issn | 1873-5118 | |
dc.identifier.uri | http://hdl.handle.net/10453/147816 | |
dc.description.abstract | Iron is essential for brain development and health where its redox properties are used for a number of neurological processes. However, iron is also a major driver of oxidative stress if not properly controlled. Brain iron distribution is highly compartmentalised and regulated by a number of proteins and small biomolecules. Here, we examine heterogeneity in regional iron levels in 10 anatomical structures from seven post-mortem human brains with no apparent neuropathology. Putamen contained the highest levels, and most case-to-case variability, of iron compared with the other regions examined. Partitioning of iron between cytosolic and membrane-bound iron was generally consistent in each region, with a slightly higher proportion (55 %) in the 'insoluble' phase. We expand on this using the Allen Human Brain Atlas to examine patterns between iron levels and transcriptomic expression of iron regulatory proteins and using quantitative size exclusion chromatography-inductively coupled plasma-mass spectrometry to assess regional differences in the molecular masses to which cytosolic iron predominantly binds. Approximately 60 % was associated with ferritin, equating to approximately 25 % of total tissue iron essentially in storage. This study is the first of its kind in human brain tissue, providing a valuable resource and new insight for iron biologists and neuroscientists, alike. | |
dc.format | Print-Electronic | |
dc.language | eng | |
dc.publisher | PERGAMON-ELSEVIER SCIENCE LTD | |
dc.relation.ispartof | Progress in neurobiology | |
dc.relation.isbasedon | 10.1016/j.pneurobio.2019.101744 | |
dc.rights | info:eu-repo/semantics/closedAccess | |
dc.subject | 1109 Neurosciences, 1701 Psychology, 1702 Cognitive Sciences | |
dc.subject.classification | Neurology & Neurosurgery | |
dc.subject.mesh | Brain | |
dc.subject.mesh | Cell Membrane | |
dc.subject.mesh | Cytosol | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Iron | |
dc.subject.mesh | RNA, Messenger | |
dc.subject.mesh | Autopsy | |
dc.subject.mesh | Spectrophotometry, Atomic | |
dc.subject.mesh | Aged | |
dc.subject.mesh | Aged, 80 and over | |
dc.subject.mesh | Middle Aged | |
dc.subject.mesh | Tissue Banks | |
dc.subject.mesh | Female | |
dc.subject.mesh | Male | |
dc.subject.mesh | Ferritins | |
dc.subject.mesh | Transcriptome | |
dc.subject.mesh | Cervical Cord | |
dc.subject.mesh | Aged | |
dc.subject.mesh | Aged, 80 and over | |
dc.subject.mesh | Autopsy | |
dc.subject.mesh | Brain | |
dc.subject.mesh | Cell Membrane | |
dc.subject.mesh | Cervical Cord | |
dc.subject.mesh | Cytosol | |
dc.subject.mesh | Female | |
dc.subject.mesh | Ferritins | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Iron | |
dc.subject.mesh | Male | |
dc.subject.mesh | Middle Aged | |
dc.subject.mesh | RNA, Messenger | |
dc.subject.mesh | Spectrophotometry, Atomic | |
dc.subject.mesh | Tissue Banks | |
dc.subject.mesh | Transcriptome | |
dc.title | Regional iron distribution and soluble ferroprotein profiles in the healthy human brain. | |
dc.type | Journal Article | |
utslib.citation.volume | 186 | |
utslib.location.activity | England | |
utslib.for | 1109 Neurosciences | |
utslib.for | 1109 Neurosciences | |
utslib.for | 1701 Psychology | |
utslib.for | 1702 Cognitive Sciences | |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences | |
utslib.copyright.status | closed_access | * |
dc.date.updated | 2021-04-03T03:19:57Z | |
pubs.publication-status | Published | |
pubs.volume | 186 |
Abstract:
Iron is essential for brain development and health where its redox properties are used for a number of neurological processes. However, iron is also a major driver of oxidative stress if not properly controlled. Brain iron distribution is highly compartmentalised and regulated by a number of proteins and small biomolecules. Here, we examine heterogeneity in regional iron levels in 10 anatomical structures from seven post-mortem human brains with no apparent neuropathology. Putamen contained the highest levels, and most case-to-case variability, of iron compared with the other regions examined. Partitioning of iron between cytosolic and membrane-bound iron was generally consistent in each region, with a slightly higher proportion (55 %) in the 'insoluble' phase. We expand on this using the Allen Human Brain Atlas to examine patterns between iron levels and transcriptomic expression of iron regulatory proteins and using quantitative size exclusion chromatography-inductively coupled plasma-mass spectrometry to assess regional differences in the molecular masses to which cytosolic iron predominantly binds. Approximately 60 % was associated with ferritin, equating to approximately 25 % of total tissue iron essentially in storage. This study is the first of its kind in human brain tissue, providing a valuable resource and new insight for iron biologists and neuroscientists, alike.
Please use this identifier to cite or link to this item:
Download statistics for the last 12 months
Not enough data to produce graph