A microRNA in a multiple-turnover RNAi enzyme complex
- Publication Type:
- Journal Article
- Citation:
- Science, 2002, 297 (5589), pp. 2056 - 2060
- Issue Date:
- 2002-09-20
Closed Access
Filename | Description | Size | |||
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2010003462OK.pdf | 126.89 kB | Adobe PDF |
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author |
Hutvágner, G https://orcid.org/0000-0002-7231-9446 |
en_US |
dc.contributor.author | Zamore, PD | en_US |
dc.date.issued | 2002-09-20 | en_US |
dc.identifier.citation | Science, 2002, 297 (5589), pp. 2056 - 2060 | en_US |
dc.identifier.issn | 0036-8075 | en_US |
dc.identifier.uri | http://hdl.handle.net/10453/14926 | |
dc.description.abstract | In animals, the double-stranded RNA-specific endonuclease Dicer produces two classes of functionally distinct, tiny RNAs: microRNAs (miRNAs) and small interfering RNAs (siRNAs). miRNAs regulate mRNA translation, whereas siRNAs direct RNA destruction via the RNA interference (RNAi) pathway. Here we show that, in human cell extracts, the miRNA let-7 naturally enters the RNAi pathway, which suggests that only the degree of complementarity between a miRNA and its RNA target determines its function. Human let-7 is a component of a previously identified, miRNA-containing ribonucleoprotein particle, which we show is an RNAi enzyme complex. Each let-7-containing complex directs multiple rounds of RNA cleavage, which explains the remarkable efficiency of the RNAi pathway in human cells. | en_US |
dc.relation.ispartof | Science | en_US |
dc.relation.isbasedon | 10.1126/science.1073827 | en_US |
dc.subject.classification | General Science & Technology | en_US |
dc.subject.mesh | Hela Cells | en_US |
dc.subject.mesh | Cytoplasm | en_US |
dc.subject.mesh | Animals | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Drosophila melanogaster | en_US |
dc.subject.mesh | Endoribonucleases | en_US |
dc.subject.mesh | RNA-Induced Silencing Complex | en_US |
dc.subject.mesh | Ribonuclease III | en_US |
dc.subject.mesh | RNA Helicases | en_US |
dc.subject.mesh | Ribonucleoproteins | en_US |
dc.subject.mesh | Ribonucleoproteins, Small Nuclear | en_US |
dc.subject.mesh | Nuclear Proteins | en_US |
dc.subject.mesh | Peptide Initiation Factors | en_US |
dc.subject.mesh | Eukaryotic Initiation Factors | en_US |
dc.subject.mesh | Eukaryotic Initiation Factor-2 | en_US |
dc.subject.mesh | RNA, Antisense | en_US |
dc.subject.mesh | MicroRNAs | en_US |
dc.subject.mesh | RNA, Small Interfering | en_US |
dc.subject.mesh | RNA, Double-Stranded | en_US |
dc.subject.mesh | RNA, Messenger | en_US |
dc.subject.mesh | RNA, Untranslated | en_US |
dc.subject.mesh | Adenosine Triphosphate | en_US |
dc.subject.mesh | Cell Extracts | en_US |
dc.subject.mesh | Minor Histocompatibility Antigens | en_US |
dc.subject.mesh | Protein Biosynthesis | en_US |
dc.subject.mesh | Gene Silencing | en_US |
dc.subject.mesh | Base Sequence | en_US |
dc.subject.mesh | Base Pairing | en_US |
dc.subject.mesh | Models, Genetic | en_US |
dc.subject.mesh | DEAD-box RNA Helicases | en_US |
dc.subject.mesh | DEAD Box Protein 20 | en_US |
dc.subject.mesh | Argonaute Proteins | en_US |
dc.subject.mesh | HeLa Cells | en_US |
dc.title | A microRNA in a multiple-turnover RNAi enzyme complex | en_US |
dc.type | Journal Article | |
utslib.citation.volume | 5589 | en_US |
utslib.citation.volume | 297 | en_US |
utslib.for | 060107 Enzymes | en_US |
dc.location.activity | ISI:000178078500048 | en_US |
pubs.embargo.period | Not known | en_US |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering | |
pubs.organisational-group | /University of Technology Sydney/Strength - CHT - Health Technologies | |
utslib.copyright.status | closed_access | |
pubs.issue | 5589 | en_US |
pubs.publication-status | Published | en_US |
pubs.volume | 297 | en_US |
Abstract:
In animals, the double-stranded RNA-specific endonuclease Dicer produces two classes of functionally distinct, tiny RNAs: microRNAs (miRNAs) and small interfering RNAs (siRNAs). miRNAs regulate mRNA translation, whereas siRNAs direct RNA destruction via the RNA interference (RNAi) pathway. Here we show that, in human cell extracts, the miRNA let-7 naturally enters the RNAi pathway, which suggests that only the degree of complementarity between a miRNA and its RNA target determines its function. Human let-7 is a component of a previously identified, miRNA-containing ribonucleoprotein particle, which we show is an RNAi enzyme complex. Each let-7-containing complex directs multiple rounds of RNA cleavage, which explains the remarkable efficiency of the RNAi pathway in human cells.
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