Spatial distribution of manganese in enamel and coronal dentine of human primary teeth

Arora, M; Hare, D; Austin, C; Smith, DR; Doble, P

Spatial distribution of manganese in enamel and coronal dentine of human primary teeth

Arora, M Hare, D

Austin, C Smith, DR Doble, P

Permalink

Publication Type:: Journal Article
Citation:: Science of the Total Environment, 2011, 409 (7), pp. 1315 - 1319
Issue Date:: 2011-03-01

Closed Access

	Filename	Description	Size
	2010004742OK.pdf		428.32 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Arora, M	en_US
dc.contributor.author	Hare, D https://orcid.org/0000-0002-5922-7643	en_US
dc.contributor.author	Austin, C	en_US
dc.contributor.author	Smith, DR	en_US
dc.contributor.author	Doble, P https://orcid.org/0000-0002-8472-1301	en_US
dc.date.available	2010-12-06	en_US
dc.date.issued	2011-03-01	en_US
dc.identifier.citation	Science of the Total Environment, 2011, 409 (7), pp. 1315 - 1319	en_US
dc.identifier.issn	0048-9697	en_US
dc.identifier.uri	http://hdl.handle.net/10453/14942
dc.description.abstract	Emerging evidence indicates that excessive exposure to manganese (Mn) during the prenatal period and early childhood may result in neurodevelopmental deficits. However, accurate exposure biomarkers are not well established, limiting our understanding of exposure-response relationships over these susceptible periods of development. Naturally shed deciduous teeth are potentially a useful biomarker of environmental exposure to Mn. However, the uptake and distribution of Mn in human teeth has not been studied in detail. Mn distribution was measured at high resolution (~. 20 μm) in eight human primary teeth using laser ablation-inductively coupled plasma-mass spectrometry. A bio-imaging methodology was applied to construct detailed elemental maps of three incisors, and bone meal (NIST SRM 1486) was used to validate the analyses. The distribution of Mn in enamel and coronal dentine showed a distinct and reproducible pattern. In enamel, the 55Mn:43Ca ratio was highest at the outer edge of enamel (range=0.57 to 4.74) for approximately 20-40μm but was substantially lower in deeper layers (range=0.005 to 0.013). The highest levels of Mn were observed in dentine immediately adjacent the pulpal margin (55Mn:43Ca range=2.27 to 6.95). Importantly, a clearly demarcated high Mn zone was observed in dentine at the incisal end of the teeth. Using confocal laser scanning microscopy to visualize the neonatal line, this region was identified as being in the prenatally formed dentine. The high-resolution map of the spatial distribution of Mn in human primary teeth highlighted specific reproducible patterns of Mn distribution in enamel and coronal dentine. © 2010 Elsevier B.V.	en_US
dc.relation.ispartof	Science of the Total Environment	en_US
dc.relation.isbasedon	10.1016/j.scitotenv.2010.12.018	en_US
dc.subject.classification	Environmental Sciences	en_US
dc.subject.mesh	Tooth, Deciduous	en_US
dc.subject.mesh	Dental Enamel	en_US
dc.subject.mesh	Dentin	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Manganese	en_US
dc.subject.mesh	Biological Markers	en_US
dc.subject.mesh	Environmental Pollutants	en_US
dc.subject.mesh	Environmental Monitoring	en_US
dc.subject.mesh	Mass Spectrometry	en_US
dc.subject.mesh	Biomarkers	en_US
dc.title	Spatial distribution of manganese in enamel and coronal dentine of human primary teeth	en_US
dc.type	Journal Article
utslib.citation.volume	7	en_US
utslib.citation.volume	409	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
dc.location.activity	WOS:000287833600012	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
utslib.copyright.status	closed_access
pubs.issue	7	en_US
pubs.publication-status	Published	en_US
pubs.volume	409	en_US

Abstract:

Emerging evidence indicates that excessive exposure to manganese (Mn) during the prenatal period and early childhood may result in neurodevelopmental deficits. However, accurate exposure biomarkers are not well established, limiting our understanding of exposure-response relationships over these susceptible periods of development. Naturally shed deciduous teeth are potentially a useful biomarker of environmental exposure to Mn. However, the uptake and distribution of Mn in human teeth has not been studied in detail. Mn distribution was measured at high resolution (~. 20 μm) in eight human primary teeth using laser ablation-inductively coupled plasma-mass spectrometry. A bio-imaging methodology was applied to construct detailed elemental maps of three incisors, and bone meal (NIST SRM 1486) was used to validate the analyses. The distribution of Mn in enamel and coronal dentine showed a distinct and reproducible pattern. In enamel, the 55Mn:43Ca ratio was highest at the outer edge of enamel (range=0.57 to 4.74) for approximately 20-40μm but was substantially lower in deeper layers (range=0.005 to 0.013). The highest levels of Mn were observed in dentine immediately adjacent the pulpal margin (55Mn:43Ca range=2.27 to 6.95). Importantly, a clearly demarcated high Mn zone was observed in dentine at the incisal end of the teeth. Using confocal laser scanning microscopy to visualize the neonatal line, this region was identified as being in the prenatally formed dentine. The high-resolution map of the spatial distribution of Mn in human primary teeth highlighted specific reproducible patterns of Mn distribution in enamel and coronal dentine. © 2010 Elsevier B.V.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/14942