Improved granulocyte colony-stimulating factor mobilization of hemopoietic progenitors using cytokine combinations in primates

Larsen, SR; Chng, K; Battah, F; Martiniello-Wilks, R; Rasko, JEJ

Improved granulocyte colony-stimulating factor mobilization of hemopoietic progenitors using cytokine combinations in primates

Larsen, SR Chng, K Battah, F Martiniello-Wilks, R

Rasko, JEJ

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Publication Type:: Journal Article
Citation:: Stem Cells, 2008, 26 (11), pp. 2974 - 2980
Issue Date:: 2008-11-01

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Field	Value	Language
dc.contributor.author	Larsen, SR	en_US
dc.contributor.author	Chng, K	en_US
dc.contributor.author	Battah, F	en_US
dc.contributor.author	Martiniello-Wilks, R https://orcid.org/0000-0002-0038-3430	en_US
dc.contributor.author	Rasko, JEJ	en_US
dc.date.issued	2008-11-01	en_US
dc.identifier.citation	Stem Cells, 2008, 26 (11), pp. 2974 - 2980	en_US
dc.identifier.issn	1066-5099	en_US
dc.identifier.uri	http://hdl.handle.net/10453/14969
dc.description.abstract	Peripheral blood stem cells (PBSCs), usually mobilized with granulocyte colony-stimulating factor (G-CSF) alone or in combination with chemotherapy, are the preferred source of cells for hemopoietic stem cell transplantation. Up to 25% of otherwise eligible transplant recipients fail to harvest adequate PBSCs. Therefore it is important to investigate existing and novel reagents to improve PBSC mobilization. Because of marked interindividual variation in humans, we developed a robust nonhuman primate model that allows the direct comparison of the efficacy of two PBSC mobilization regimens within the same animal. Using this model, we compared pegylated G-CSF (pegG-CSF) with standard G-CSF and compared the combination of G-CSF and pegylated megakaryocyte growth and development factor (pegMGDF) with G-CSF plus stem cell factor (SCF) by measuring the levels of CD34+ cells, colony-forming cells (CFCs), and SCID repopulating cells (SRCs) before and after cytokine administration. Mobilization of CD34+ cells, CFCs and SRCs using pegG-CSF achieved similar levels to those resulting from 5 days of standard G-CSF. The combination of G-CSF+pegMGDF mobilized progenitors to levels similar to G-CSF+SCF but greater than standard G-CSF for CD34+ cells and CFC. This first direct comparison of PBSC mobilization in individual primates demonstrates that peg-G-CSF is equivalent to daily G-CSF and that the addition of pegMGDF to G-CSF improves mobilization. In light of the development of new thrombopoietin agonists, these data offer the potential for improved stem cell mobilization strategies. ©AlphaMed Press.	en_US
dc.relation.ispartof	Stem Cells	en_US
dc.relation.isbasedon	10.1634/stemcells.2008-0560	en_US
dc.subject.classification	Immunology	en_US
dc.subject.mesh	Hematopoietic Stem Cells	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Papio	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Polyethylene Glycols	en_US
dc.subject.mesh	Granulocyte Colony-Stimulating Factor	en_US
dc.subject.mesh	Thrombopoietin	en_US
dc.subject.mesh	Antigens, CD34	en_US
dc.subject.mesh	Cytokines	en_US
dc.subject.mesh	Hematopoietic Stem Cell Mobilization	en_US
dc.subject.mesh	Female	en_US
dc.subject.mesh	Male	en_US
dc.title	Improved granulocyte colony-stimulating factor mobilization of hemopoietic progenitors using cytokine combinations in primates	en_US
dc.type	Journal Article
utslib.citation.volume	11	en_US
utslib.citation.volume	26	en_US
utslib.for	0699 Other Biological Sciences	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	10 Technology	en_US
utslib.for	11 Medical and Health Sciences	en_US
dc.location.activity	ISI:000261156500028	en_US
dc.location.activity	London, UK
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.issue	11	en_US
pubs.publication-status	Published	en_US
pubs.volume	26	en_US

Abstract:

Peripheral blood stem cells (PBSCs), usually mobilized with granulocyte colony-stimulating factor (G-CSF) alone or in combination with chemotherapy, are the preferred source of cells for hemopoietic stem cell transplantation. Up to 25% of otherwise eligible transplant recipients fail to harvest adequate PBSCs. Therefore it is important to investigate existing and novel reagents to improve PBSC mobilization. Because of marked interindividual variation in humans, we developed a robust nonhuman primate model that allows the direct comparison of the efficacy of two PBSC mobilization regimens within the same animal. Using this model, we compared pegylated G-CSF (pegG-CSF) with standard G-CSF and compared the combination of G-CSF and pegylated megakaryocyte growth and development factor (pegMGDF) with G-CSF plus stem cell factor (SCF) by measuring the levels of CD34+ cells, colony-forming cells (CFCs), and SCID repopulating cells (SRCs) before and after cytokine administration. Mobilization of CD34+ cells, CFCs and SRCs using pegG-CSF achieved similar levels to those resulting from 5 days of standard G-CSF. The combination of G-CSF+pegMGDF mobilized progenitors to levels similar to G-CSF+SCF but greater than standard G-CSF for CD34+ cells and CFC. This first direct comparison of PBSC mobilization in individual primates demonstrates that peg-G-CSF is equivalent to daily G-CSF and that the addition of pegMGDF to G-CSF improves mobilization. In light of the development of new thrombopoietin agonists, these data offer the potential for improved stem cell mobilization strategies. ©AlphaMed Press.

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http://hdl.handle.net/10453/14969