Microbial analysis for the ammonium removal from landfill leachate in an aerobic granular sludge sequencing batch reactor.

Wei, Y; Ye, Y; Ji, M; Peng, S; Qin, F; Guo, W; Ngo, HH

Microbial analysis for the ammonium removal from landfill leachate in an aerobic granular sludge sequencing batch reactor.

Wei, Y Ye, Y Ji, M Peng, S Qin, F Guo, W

Ngo, HH

Permalink

Publisher:: Elsevier BV
Publication Type:: Journal Article
Citation:: Bioresource technology, 2021, 324, pp. 124639
Issue Date:: 2021-03

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

The embargo period expires on 1 Mar 2023

Adobe PDF

Download Accepted ManuscriptAdobe PDF (256.23 kB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Wei, Y
dc.contributor.author	Ye, Y
dc.contributor.author	Ji, M
dc.contributor.author	Peng, S
dc.contributor.author	Qin, F
dc.contributor.author	Guo, W https://orcid.org/0000-0001-5542-2858
dc.contributor.author	Ngo, HH
dc.date.accessioned	2021-06-22T02:26:18Z
dc.date.available	2020-12-26
dc.date.available	2021-06-22T02:26:18Z
dc.date.issued	2021-03
dc.identifier.citation	Bioresource technology, 2021, 324, pp. 124639
dc.identifier.issn	0960-8524
dc.identifier.issn	1873-2976
dc.identifier.uri	http://hdl.handle.net/10453/149700
dc.description.abstract	In this study, a laboratory-scale sequencing batch reactor (SBR) equipped with aerobic granular sludge (AGS) technology was continuously operated for 220 days to remove ammonium from an existing landfill leachate. The ammonium removal was characterized by polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) technology. This method helped to analyze the long-term community structural stability of ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB) and denitrifying bacteria (DB) throughout the experiment. Simultaneously, 16S rRNA gene cloning and sequencing analysis identified the dominant species of different microbial species. Experimental results confirmed that ammonium removal was inhibited at the high nitrogen loading rate (NLR) stage while the low NLR stage achieved satisfactory ammonium removal. Moreover, the findings demonstrated that functionally stable wastewater treatment bioreactors facilitated the occurrence of stable microbial community structures.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Elsevier BV
dc.relation.ispartof	Bioresource technology
dc.relation.isbasedon	10.1016/j.biortech.2020.124639
dc.rights	info:eu-repo/semantics/embargoedAccess
dc.subject.classification	Biotechnology
dc.subject.mesh	Nitrogen
dc.subject.mesh	Ammonium Compounds
dc.subject.mesh	RNA, Ribosomal, 16S
dc.subject.mesh	Water Pollutants, Chemical
dc.subject.mesh	Bioreactors
dc.subject.mesh	Sewage
dc.subject.mesh	Ammonium Compounds
dc.subject.mesh	Bioreactors
dc.subject.mesh	Nitrogen
dc.subject.mesh	RNA, Ribosomal, 16S
dc.subject.mesh	Sewage
dc.subject.mesh	Water Pollutants, Chemical
dc.title	Microbial analysis for the ammonium removal from landfill leachate in an aerobic granular sludge sequencing batch reactor.
dc.type	Journal Article
utslib.citation.volume	324
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	open_access	*
utslib.copyright.embargo	2023-03-01T00:00:00+1000Z
dc.date.updated	2021-06-22T02:26:17Z
pubs.publication-status	Published
pubs.volume	324

Abstract:

In this study, a laboratory-scale sequencing batch reactor (SBR) equipped with aerobic granular sludge (AGS) technology was continuously operated for 220 days to remove ammonium from an existing landfill leachate. The ammonium removal was characterized by polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) technology. This method helped to analyze the long-term community structural stability of ammonia-oxidizing bacteria (AOB), nitrite-oxidizing bacteria (NOB) and denitrifying bacteria (DB) throughout the experiment. Simultaneously, 16S rRNA gene cloning and sequencing analysis identified the dominant species of different microbial species. Experimental results confirmed that ammonium removal was inhibited at the high nitrogen loading rate (NLR) stage while the low NLR stage achieved satisfactory ammonium removal. Moreover, the findings demonstrated that functionally stable wastewater treatment bioreactors facilitated the occurrence of stable microbial community structures.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/149700