Enhanced biocatalysis of phenanthrene in aqueous phase by novel CA-Ca-SBE-laccase biocatalyst: Performance and mechanism

Chen, X; Hu, Z; Xie, H; Ngo, HH; Guo, W; Zhang, J

Enhanced biocatalysis of phenanthrene in aqueous phase by novel CA-Ca-SBE-laccase biocatalyst: Performance and mechanism

Chen, X Hu, Z Xie, H Ngo, HH Guo, W

Zhang, J

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Publisher:: Elsevier BV
Publication Type:: Journal Article
Citation:: Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2021, 611, pp. 125884-125884
Issue Date:: 2021-02-20

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Field	Value	Language
dc.contributor.author	Chen, X
dc.contributor.author	Hu, Z
dc.contributor.author	Xie, H
dc.contributor.author	Ngo, HH
dc.contributor.author	Guo, W https://orcid.org/0000-0001-5542-2858
dc.contributor.author	Zhang, J
dc.date.accessioned	2021-06-22T02:29:10Z
dc.date.available	2021-06-22T02:29:10Z
dc.date.issued	2021-02-20
dc.identifier.citation	Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2021, 611, pp. 125884-125884
dc.identifier.issn	0927-7757
dc.identifier.issn	1873-4359
dc.identifier.uri	http://hdl.handle.net/10453/149701
dc.description.abstract	Enzymes-mediated biocatalysis is believed to be a promising strategy for the degradation of polycyclic aromatic hydrocarbon (e.g., phenanthrene) in aqueous phase, due to its high efficiency and environmental harmfulness. However, the reusability of enzymes-mediated biocatalysis and its requirement for redox mediators limited its application. In this paper, enhanced phenanthrene removal was achieved by using a novel biocatalyst which coencapsulating laccase and natural redox mediator soybean meal extract on calcium modified chitosan-alginate (CA-Ca-SBE-laccase) beads with a network pore structure. High degradation efficiency of 94.4 % was achieved after treating aqueous solutions of 0.5 ppm phenanthrene for 20 min with the CA-Ca-SBE-laccase beads, which was 20–30 % higher than that of free laccase and ordinary immobilized laccase beads. Both the radical pathway of oxygen-containing stable free radicals and the nonradical laccase-SBE combined substrates with higher redox potential generated in CA-Ca-SBE-laccase beads contributed to the degradation of phenanthrene, and achieved the maximum double synergy of mediator and laccase. Benefiting from redox-mediating encapsulated SBE and from Ca modification of chitosan, the biocatalyst maintained fairly stable catalytic activities over a wide range of pH values, and considerable repeatability and storage stability were obtained. This study put forward an efficient laccase, redox mediator and immobilized support cofunctionalization technology, and would provide a new perspective for the increasing application scope of enzymes-mediated biocatalysis.
dc.language	en
dc.publisher	Elsevier BV
dc.relation.ispartof	Colloids and Surfaces A: Physicochemical and Engineering Aspects
dc.relation.isbasedon	10.1016/j.colsurfa.2020.125884
dc.rights	info:eu-repo/semantics/embargoedAccess
dc.subject	02 Physical Sciences, 03 Chemical Sciences, 09 Engineering
dc.subject.classification	Chemical Physics
dc.title	Enhanced biocatalysis of phenanthrene in aqueous phase by novel CA-Ca-SBE-laccase biocatalyst: Performance and mechanism
dc.type	Journal Article
utslib.citation.volume	611
utslib.for	02 Physical Sciences
utslib.for	03 Chemical Sciences
utslib.for	09 Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	open_access	*
pubs.consider-herdc	false
utslib.copyright.embargo	2023-02-20T00:00:00+1000Z
dc.date.updated	2021-06-22T02:29:08Z
pubs.publication-status	Published
pubs.volume	611

Abstract:

Enzymes-mediated biocatalysis is believed to be a promising strategy for the degradation of polycyclic aromatic hydrocarbon (e.g., phenanthrene) in aqueous phase, due to its high efficiency and environmental harmfulness. However, the reusability of enzymes-mediated biocatalysis and its requirement for redox mediators limited its application. In this paper, enhanced phenanthrene removal was achieved by using a novel biocatalyst which coencapsulating laccase and natural redox mediator soybean meal extract on calcium modified chitosan-alginate (CA-Ca-SBE-laccase) beads with a network pore structure. High degradation efficiency of 94.4 % was achieved after treating aqueous solutions of 0.5 ppm phenanthrene for 20 min with the CA-Ca-SBE-laccase beads, which was 20–30 % higher than that of free laccase and ordinary immobilized laccase beads. Both the radical pathway of oxygen-containing stable free radicals and the nonradical laccase-SBE combined substrates with higher redox potential generated in CA-Ca-SBE-laccase beads contributed to the degradation of phenanthrene, and achieved the maximum double synergy of mediator and laccase. Benefiting from redox-mediating encapsulated SBE and from Ca modification of chitosan, the biocatalyst maintained fairly stable catalytic activities over a wide range of pH values, and considerable repeatability and storage stability were obtained. This study put forward an efficient laccase, redox mediator and immobilized support cofunctionalization technology, and would provide a new perspective for the increasing application scope of enzymes-mediated biocatalysis.

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http://hdl.handle.net/10453/149701