Alteromonas macleodii 0444 and Neptunomonas sp. 0536, two novel probiotics for hatchery-reared Greenshell™ mussel larvae, Perna canaliculus

Kesarcodi-Watson, A; Kaspar, H; Lategan, MJ; Gibson, L

Alteromonas macleodii 0444 and Neptunomonas sp. 0536, two novel probiotics for hatchery-reared Greenshell™ mussel larvae, Perna canaliculus

Kesarcodi-Watson, A Kaspar, H Lategan, MJ Gibson, L

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Publication Type:: Journal Article
Citation:: Aquaculture, 2010, 309 (1-4), pp. 49 - 55
Issue Date:: 2010-11-22

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Field	Value	Language
dc.contributor.author	Kesarcodi-Watson, A	en_US
dc.contributor.author	Kaspar, H	en_US
dc.contributor.author	Lategan, MJ	en_US
dc.contributor.author	Gibson, L	en_US
dc.date.issued	2010-11-22	en_US
dc.identifier.citation	Aquaculture, 2010, 309 (1-4), pp. 49 - 55	en_US
dc.identifier.issn	0044-8486	en_US
dc.identifier.uri	http://hdl.handle.net/10453/15018
dc.description.abstract	Antibiotic management of aquacultured animals, such as Greenshell™ mussel (GSM) larvae, Perna canaliculus, is undesirable because of health concerns and political pressures; hence, alternatives are needed. Herein, two novel probiotic bacteria were identified and trialled in a GSM larval rearing hatchery. Sequencing of the 16S rRNA gene and phylogenetic analysis identified the strains as Alteromonas macleodii 0444 and Neptunomonas sp. 0536. Both probiotics were evaluated separately at the Glenhaven Aquaculture Centre hatchery facility during routine larval rearing and when the larvae were challenged with both a high (107 and 106CFUml-1) and low (106 and 105CFUml-1) pathogenic dose of Vibrio sp. DO1 and V. splendidus respectively. In all experiments, probiotic application improved larval survival significantly when administered prior to pathogen exposure. Across all experiments, larvae that were exposed to the high and low dosages of pathogens averaged 14% and 36% survival respectively on the fourth day following pathogen exposure. The administration of probiotics prior to pathogen challenge resulted in larval survival of 50% and 66% respectively. Non-inoculated control larvae and larvae administered the probiotic alone demonstrated 67% and 79% survival respectively. Neptunomonas sp. 0536 appeared to suppress naturally occurring vibrios in the culture environment of healthy GSM larvae. This is the first report of A. macleodii and Neptunomonas sp. as probiotic bacteria in a large scale production facility. © 2010 Elsevier B.V.	en_US
dc.relation.ispartof	Aquaculture	en_US
dc.relation.isbasedon	10.1016/j.aquaculture.2010.09.019	en_US
dc.subject.classification	Fisheries	en_US
dc.title	Alteromonas macleodii 0444 and Neptunomonas sp. 0536, two novel probiotics for hatchery-reared Greenshell™ mussel larvae, Perna canaliculus	en_US
dc.type	Journal Article
utslib.citation.volume	1-4	en_US
utslib.citation.volume	309	en_US
utslib.for	0704 Fisheries Sciences	en_US
utslib.for	0608 Zoology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	closed_access
pubs.issue	1-4	en_US
pubs.publication-status	Published	en_US
pubs.volume	309	en_US

Abstract:

Antibiotic management of aquacultured animals, such as Greenshell™ mussel (GSM) larvae, Perna canaliculus, is undesirable because of health concerns and political pressures; hence, alternatives are needed. Herein, two novel probiotic bacteria were identified and trialled in a GSM larval rearing hatchery. Sequencing of the 16S rRNA gene and phylogenetic analysis identified the strains as Alteromonas macleodii 0444 and Neptunomonas sp. 0536. Both probiotics were evaluated separately at the Glenhaven Aquaculture Centre hatchery facility during routine larval rearing and when the larvae were challenged with both a high (107 and 106CFUml-1) and low (106 and 105CFUml-1) pathogenic dose of Vibrio sp. DO1 and V. splendidus respectively. In all experiments, probiotic application improved larval survival significantly when administered prior to pathogen exposure. Across all experiments, larvae that were exposed to the high and low dosages of pathogens averaged 14% and 36% survival respectively on the fourth day following pathogen exposure. The administration of probiotics prior to pathogen challenge resulted in larval survival of 50% and 66% respectively. Non-inoculated control larvae and larvae administered the probiotic alone demonstrated 67% and 79% survival respectively. Neptunomonas sp. 0536 appeared to suppress naturally occurring vibrios in the culture environment of healthy GSM larvae. This is the first report of A. macleodii and Neptunomonas sp. as probiotic bacteria in a large scale production facility. © 2010 Elsevier B.V.

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http://hdl.handle.net/10453/15018