Bioengineering a Light-Responsive Encapsulin Nanoreactor: A Potential Tool for <i>In Vitro</i> Photodynamic Therapy.

Diaz, D; Vidal, X; Sunna, A; Care, A

Bioengineering a Light-Responsive Encapsulin Nanoreactor: A Potential Tool for <i>In Vitro</i> Photodynamic Therapy.

Diaz, D Vidal, X Sunna, A Care, A

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Publisher:: AMER CHEMICAL SOC
Publication Type:: Journal Article
Citation:: ACS applied materials & interfaces, 2021, 13, (7), pp. 7977-7986
Issue Date:: 2021-02-15

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Field	Value	Language
dc.contributor.author	Diaz, D
dc.contributor.author	Vidal, X
dc.contributor.author	Sunna, A
dc.contributor.author	Care, A https://orcid.org/0000-0002-0035-7961
dc.date.accessioned	2021-09-15T22:41:01Z
dc.date.available	2021-09-15T22:41:01Z
dc.date.issued	2021-02-15
dc.identifier.citation	ACS applied materials & interfaces, 2021, 13, (7), pp. 7977-7986
dc.identifier.issn	1944-8244
dc.identifier.issn	1944-8252
dc.identifier.uri	http://hdl.handle.net/10453/150570
dc.description.abstract	Encapsulins, a prokaryotic class of self-assembling protein nanocompartments, are being re-engineered to serve as "nanoreactors" for the augmentation or creation of key biochemical reactions. However, approaches that allow encapsulin nanoreactors to be functionally activated with spatial and temporal precision are lacking. We report the construction of a light-responsive encapsulin nanoreactor for "on demand" production of reactive oxygen species (ROS). Herein, encapsulins were loaded with the fluorescent flavoprotein mini-singlet oxygen generator (miniSOG), a biological photosensitizer that is activated by blue light to generate ROS, primarily singlet oxygen (<sup>1</sup>O<sub>2</sub>). We established that the nanocompartments stably encased miniSOG and in response to blue light were able to mediate the photoconversion of molecular oxygen into ROS. Using an <i>in vitro</i> model of lung cancer, we showed that ROS generated by the nanoreactor triggered photosensitized oxidation reactions which exerted a toxic effect on tumor cells, suggesting utility in photodynamic therapy. This encapsulin nanoreactor thus represents a platform for the light-controlled initiation and/or modulation of ROS-driven processes in biomedicine and biotechnology.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	AMER CHEMICAL SOC
dc.relation	http://purl.org/au-research/grants/arc/CE140100003
dc.relation.ispartof	ACS applied materials & interfaces
dc.relation.isbasedon	10.1021/acsami.0c21141
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	03 Chemical Sciences, 09 Engineering
dc.subject.classification	Nanoscience & Nanotechnology
dc.subject.mesh	Humans
dc.subject.mesh	Lung Neoplasms
dc.subject.mesh	Singlet Oxygen
dc.subject.mesh	Reactive Oxygen Species
dc.subject.mesh	Flavoproteins
dc.subject.mesh	Antineoplastic Agents
dc.subject.mesh	Photosensitizing Agents
dc.subject.mesh	Fluorescent Dyes
dc.subject.mesh	Photochemotherapy
dc.subject.mesh	Spectrometry, Fluorescence
dc.subject.mesh	Drug Screening Assays, Antitumor
dc.subject.mesh	Biomedical Engineering
dc.subject.mesh	Cell Proliferation
dc.subject.mesh	Cell Survival
dc.subject.mesh	Particle Size
dc.subject.mesh	Surface Properties
dc.subject.mesh	Light
dc.subject.mesh	Nanocomposites
dc.subject.mesh	A549 Cells
dc.subject.mesh	A549 Cells
dc.subject.mesh	Antineoplastic Agents
dc.subject.mesh	Biomedical Engineering
dc.subject.mesh	Cell Proliferation
dc.subject.mesh	Cell Survival
dc.subject.mesh	Drug Screening Assays, Antitumor
dc.subject.mesh	Flavoproteins
dc.subject.mesh	Fluorescent Dyes
dc.subject.mesh	Humans
dc.subject.mesh	Light
dc.subject.mesh	Lung Neoplasms
dc.subject.mesh	Nanocomposites
dc.subject.mesh	Particle Size
dc.subject.mesh	Photochemotherapy
dc.subject.mesh	Photosensitizing Agents
dc.subject.mesh	Reactive Oxygen Species
dc.subject.mesh	Singlet Oxygen
dc.subject.mesh	Spectrometry, Fluorescence
dc.subject.mesh	Surface Properties
dc.title	Bioengineering a Light-Responsive Encapsulin Nanoreactor: A Potential Tool for <i>In Vitro</i> Photodynamic Therapy.
dc.type	Journal Article
utslib.citation.volume	13
utslib.location.activity	United States
utslib.for	03 Chemical Sciences
utslib.for	09 Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access	*
dc.date.updated	2021-09-15T22:40:58Z
pubs.issue	7
pubs.publication-status	Published
pubs.volume	13
utslib.citation.issue	7

Abstract:

Encapsulins, a prokaryotic class of self-assembling protein nanocompartments, are being re-engineered to serve as "nanoreactors" for the augmentation or creation of key biochemical reactions. However, approaches that allow encapsulin nanoreactors to be functionally activated with spatial and temporal precision are lacking. We report the construction of a light-responsive encapsulin nanoreactor for "on demand" production of reactive oxygen species (ROS). Herein, encapsulins were loaded with the fluorescent flavoprotein mini-singlet oxygen generator (miniSOG), a biological photosensitizer that is activated by blue light to generate ROS, primarily singlet oxygen (¹O₂). We established that the nanocompartments stably encased miniSOG and in response to blue light were able to mediate the photoconversion of molecular oxygen into ROS. Using an in vitro model of lung cancer, we showed that ROS generated by the nanoreactor triggered photosensitized oxidation reactions which exerted a toxic effect on tumor cells, suggesting utility in photodynamic therapy. This encapsulin nanoreactor thus represents a platform for the light-controlled initiation and/or modulation of ROS-driven processes in biomedicine and biotechnology.

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http://hdl.handle.net/10453/150570