Cysteine biosynthesis contributes to β-methylamino-l-alanine tolerance in Escherichia coli.

Italiano, CJ; Pu, L; Violi, JP; Duggin, IG; Rodgers, KJ

Cysteine biosynthesis contributes to β-methylamino-l-alanine tolerance in Escherichia coli.

Italiano, CJ Pu, L Violi, JP Duggin, IG Rodgers, KJ

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Publisher:: Elsevier BV
Publication Type:: Journal Article
Citation:: Research in microbiology, 2021, 172, (6), pp. 103852
Issue Date:: 2021-07-08

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Field	Value	Language
dc.contributor.author	Italiano, CJ
dc.contributor.author	Pu, L
dc.contributor.author	Violi, JP
dc.contributor.author	Duggin, IG
dc.contributor.author	Rodgers, KJ
dc.date.accessioned	2022-01-04T02:17:21Z
dc.date.available	2021-06-29
dc.date.available	2022-01-04T02:17:21Z
dc.date.issued	2021-07-08
dc.identifier.citation	Research in microbiology, 2021, 172, (6), pp. 103852
dc.identifier.issn	0923-2508
dc.identifier.issn	1769-7123
dc.identifier.uri	http://hdl.handle.net/10453/152640
dc.description.abstract	In contrast to mammalian cells, bacteria such as Escherichia coli have been shown to display tolerance towards the neurotoxin β-methylamino-l-alanine (BMAA) suggesting that these prokaryotes possess a way to metabolise BMAA or its products, resulting in their export, degradation, or detoxification. Single gene deletion mutants of E. coli K-12 with inactivated amino acid biosynthesis pathways were treated with 500 μg/ml BMAA and the resulting growth was monitored. Wild type E. coli and most of the gene deletion mutants displayed unaltered growth in the presence of BMAA over 12 h. Conversely, deletion of genes in the cysteine biosynthesis pathway, cysE, cysK or cysM resulted in a BMAA dose-dependent growth delay in minimal medium. Through further studies of the ΔcysE strain, we observed increased susceptibility to oxidative stress from H<sub>2</sub>O<sub>2</sub> in minimal medium, and disruptions in glutathione levels and oxidation state. The cysteine biosynthesis pathway is therefore linked to the tolerance of BMAA and oxidative stress in E. coli, which potentially represents a mechanism of BMAA detoxification.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Elsevier BV
dc.relation	http://purl.org/au-research/grants/arc/DP160105005
dc.relation	http://purl.org/au-research/grants/arc/FT160100010
dc.relation.ispartof	Research in microbiology
dc.relation.hasversion	Accepted version	en
dc.relation.isbasedon	10.1016/j.resmic.2021.103852
dc.rights	info:eu-repo/semantics/embargoedAccess
dc.subject	0605 Microbiology, 1108 Medical Microbiology
dc.subject.classification	Microbiology
dc.subject.mesh	Amino Acids, Diamino
dc.subject.mesh	Culture Media
dc.subject.mesh	Cyanobacteria Toxins
dc.subject.mesh	Cysteine
dc.subject.mesh	Cysteine Synthase
dc.subject.mesh	Drug Tolerance
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Escherichia coli Proteins
dc.subject.mesh	Gene Deletion
dc.subject.mesh	Glutathione
dc.subject.mesh	Hydrogen Peroxide
dc.subject.mesh	Metabolic Networks and Pathways
dc.subject.mesh	Oxidation-Reduction
dc.subject.mesh	Oxidative Stress
dc.subject.mesh	Serine O-Acetyltransferase
dc.title	Cysteine biosynthesis contributes to β-methylamino-l-alanine tolerance in Escherichia coli.
dc.type	Journal Article
utslib.citation.volume	172
utslib.location.activity	France
utslib.for	0605 Microbiology
utslib.for	1108 Medical Microbiology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
utslib.copyright.embargo	2022-10-21T00:00:00+1000Z
dc.date.updated	2022-01-04T02:17:13Z
pubs.issue	6
pubs.publication-status	Published
pubs.volume	172
utslib.citation.issue	6

Abstract:

In contrast to mammalian cells, bacteria such as Escherichia coli have been shown to display tolerance towards the neurotoxin β-methylamino-l-alanine (BMAA) suggesting that these prokaryotes possess a way to metabolise BMAA or its products, resulting in their export, degradation, or detoxification. Single gene deletion mutants of E. coli K-12 with inactivated amino acid biosynthesis pathways were treated with 500 μg/ml BMAA and the resulting growth was monitored. Wild type E. coli and most of the gene deletion mutants displayed unaltered growth in the presence of BMAA over 12 h. Conversely, deletion of genes in the cysteine biosynthesis pathway, cysE, cysK or cysM resulted in a BMAA dose-dependent growth delay in minimal medium. Through further studies of the ΔcysE strain, we observed increased susceptibility to oxidative stress from H₂O₂ in minimal medium, and disruptions in glutathione levels and oxidation state. The cysteine biosynthesis pathway is therefore linked to the tolerance of BMAA and oxidative stress in E. coli, which potentially represents a mechanism of BMAA detoxification.

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http://hdl.handle.net/10453/152640