A Novel Method for Creating a Synthetic L-DOPA Proteome and In Vitro Evidence of Incorporation

Steele, JR; Strange, N; Rodgers, KJ; Padula, MP

A Novel Method for Creating a Synthetic L-DOPA Proteome and In Vitro Evidence of Incorporation

Steele, JR Strange, N

Rodgers, KJ Padula, MP

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Publisher:: MDPI AG
Publication Type:: Journal Article
Citation:: Proteomes, 2021, 9, (2), pp. 1-15
Issue Date:: 2021-05-24

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Field	Value	Language
dc.contributor.author	Steele, JR
dc.contributor.author	Strange, N https://orcid.org/0000-0002-9356-8070
dc.contributor.author	Rodgers, KJ
dc.contributor.author	Padula, MP
dc.date.accessioned	2022-01-12T01:50:59Z
dc.date.available	2021-05-18
dc.date.available	2022-01-12T01:50:59Z
dc.date.issued	2021-05-24
dc.identifier.citation	Proteomes, 2021, 9, (2), pp. 1-15
dc.identifier.issn	2227-7382
dc.identifier.issn	2227-7382
dc.identifier.uri	http://hdl.handle.net/10453/152953
dc.description.abstract	Proteinopathies are protein misfolding diseases that have an underlying factor that affects the conformation of proteoforms. A factor hypothesised to play a role in these diseases is the incorporation of non-protein amino acids into proteins, with a key example being the therapeutic drug levodopa. The presence of levodopa as a protein constituent has been explored in several studies, but it has not been examined in a global proteomic manner. This paper provides a proof-of-concept method for enzymatically creating levodopa-containing proteins using the enzyme tyrosinase and provides spectral evidence of in vitro incorporation in addition to the induction of the unfolded protein response due to levodopa
dc.format	Electronic
dc.language	eng
dc.publisher	MDPI AG
dc.relation.ispartof	Proteomes
dc.relation.isbasedon	10.3390/proteomes9020024
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0601 Biochemistry and Cell Biology
dc.title	A Novel Method for Creating a Synthetic L-DOPA Proteome and In Vitro Evidence of Incorporation
dc.type	Journal Article
utslib.citation.volume	9
utslib.location.activity	Switzerland
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Provost
pubs.organisational-group	/University of Technology Sydney/Provost/Jumbunna
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
pubs.consider-herdc	false
dc.date.updated	2022-01-12T01:50:56Z
pubs.issue	2
pubs.publication-status	Published
pubs.volume	9
utslib.citation.issue	2

Abstract:

Proteinopathies are protein misfolding diseases that have an underlying factor that affects the conformation of proteoforms. A factor hypothesised to play a role in these diseases is the incorporation of non-protein amino acids into proteins, with a key example being the therapeutic drug levodopa. The presence of levodopa as a protein constituent has been explored in several studies, but it has not been examined in a global proteomic manner. This paper provides a proof-of-concept method for enzymatically creating levodopa-containing proteins using the enzyme tyrosinase and provides spectral evidence of in vitro incorporation in addition to the induction of the unfolded protein response due to levodopa

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http://hdl.handle.net/10453/152953