Langmuir-Schaefer Deposition to Create an Asymmetrical Lipopolysaccharide Sparsely Tethered Lipid Bilayer.

Cranfield, CG; Le Brun, AP; Garcia, A; Cornell, BA; Holt, SA

Langmuir-Schaefer Deposition to Create an Asymmetrical Lipopolysaccharide Sparsely Tethered Lipid Bilayer.

Cranfield, CG Le Brun, AP Garcia, A

Cornell, BA Holt, SA

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Publisher:: Springer US
Publication Type:: Chapter
Citation:: Methods in Molecular Biology, 2022, 2402, pp. 21-30
Issue Date:: 2022

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Field	Value	Language
dc.contributor.author	Cranfield, CG
dc.contributor.author	Le Brun, AP
dc.contributor.author	Garcia, A https://orcid.org/0000-0002-1159-4567
dc.contributor.author	Cornell, BA
dc.contributor.author	Holt, SA
dc.date.accessioned	2022-01-12T06:24:27Z
dc.date.available	2022-01-12T06:24:27Z
dc.date.issued	2022
dc.identifier.citation	Methods in Molecular Biology, 2022, 2402, pp. 21-30
dc.identifier.isbn	9781071618424
dc.identifier.uri	http://hdl.handle.net/10453/152993
dc.description.abstract	Because they are firmly anchored to a noble metal substrate, tethered bilayer lipid membranes (tBLMs) are considerably more robust than supported lipid bilayers such as black lipid membranes (BLMs) (Cranfield et al. Biophys J 106:182-189, 2014). The challenge to rapidly create asymmetrical tBLMs that include a lipopolysaccharide outer leaflet for bacterial model membrane research can be overcome by the use of a Langmuir-Schaefer deposition protocol. Here, we describe the procedures required to assemble and test asymmetric lipopolysaccharide (LPS) tethered lipid bilayers.
dc.format	Print
dc.language	en
dc.publisher	Springer US
dc.relation.ispartof	Methods in Molecular Biology
dc.relation.hasversion	Submitted version	en
dc.relation.isbasedon	10.1007/978-1-0716-1843-1_3
dc.rights	info:eu-repo/semantics/embargoedAccess
dc.rights	This is a post-peer-review, pre-copyedit version of a chapter published in Membrane Lipids. The final authenticated version is available online at: https://doi.org/10.1007/978-1-0716-1843-1_3	en
dc.subject	0399 Other Chemical Sciences, 0601 Biochemistry and Cell Biology
dc.subject.classification	Developmental Biology
dc.title	Langmuir-Schaefer Deposition to Create an Asymmetrical Lipopolysaccharide Sparsely Tethered Lipid Bilayer.
dc.type	Chapter
utslib.citation.volume	2402
utslib.for	0399 Other Chemical Sciences
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Centre for Health Technologies (CHT)
utslib.copyright.status	open_access	*
utslib.copyright.embargo	2023-12-02T00:00:00+1000Z
dc.date.updated	2022-01-12T06:24:26Z
pubs.publication-status	Published
pubs.volume	2402

Abstract:

Because they are firmly anchored to a noble metal substrate, tethered bilayer lipid membranes (tBLMs) are considerably more robust than supported lipid bilayers such as black lipid membranes (BLMs) (Cranfield et al. Biophys J 106:182-189, 2014). The challenge to rapidly create asymmetrical tBLMs that include a lipopolysaccharide outer leaflet for bacterial model membrane research can be overcome by the use of a Langmuir-Schaefer deposition protocol. Here, we describe the procedures required to assemble and test asymmetric lipopolysaccharide (LPS) tethered lipid bilayers.

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http://hdl.handle.net/10453/152993