Small RNA asymmetry in RNAi: Function in RISC assembly and gene regulation

Hutvagner, G

Small RNA asymmetry in RNAi: Function in RISC assembly and gene regulation

Hutvagner, G

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Publication Type:: Journal Article
Citation:: FEBS Letters, 2005, 579 (26), pp. 5850 - 5857
Issue Date:: 2005-10-31

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Field	Value	Language
dc.contributor.author	Hutvagner, G https://orcid.org/0000-0002-7231-9446	en_US
dc.date.available	2005-08-31	en_US
dc.date.issued	2005-10-31	en_US
dc.identifier.citation	FEBS Letters, 2005, 579 (26), pp. 5850 - 5857	en_US
dc.identifier.issn	0014-5793	en_US
dc.identifier.uri	http://hdl.handle.net/10453/15313
dc.description.abstract	RNAi is a conserved gene-specific regulatory mechanism, which silences target gene expression transcriptionally and post-transcriptionally. The RNAi machinery converts the sequence specific information of a long double stranded RNAs (dsRNAs) into small 21-22 nt long dsRNAs (siRNAs, miRNAs) which assemble into an effector complex, the RNA induced silencing complex (RISC). RISC assembly is asymmetric; one strand of an siRNA or a miRNA preferentially incorporates into the RNA-protein complex. Here, I review the rules of the asymmetric RISC formation and discuss their possible regulatory function in several steps in RNAi. © 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.	en_US
dc.relation.ispartof	FEBS Letters	en_US
dc.relation.isbasedon	10.1016/j.febslet.2005.08.071	en_US
dc.subject.classification	Biochemistry & Molecular Biology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	RNA-Induced Silencing Complex	en_US
dc.subject.mesh	MicroRNAs	en_US
dc.subject.mesh	RNA, Small Interfering	en_US
dc.subject.mesh	RNA, Double-Stranded	en_US
dc.subject.mesh	Biophysics	en_US
dc.subject.mesh	Transcription, Genetic	en_US
dc.subject.mesh	Gene Expression Regulation	en_US
dc.subject.mesh	RNA Interference	en_US
dc.subject.mesh	RNA Processing, Post-Transcriptional	en_US
dc.subject.mesh	Base Sequence	en_US
dc.subject.mesh	Thermodynamics	en_US
dc.subject.mesh	Models, Genetic	en_US
dc.subject.mesh	Models, Chemical	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.subject.mesh	Biophysical Phenomena	en_US
dc.title	Small RNA asymmetry in RNAi: Function in RISC assembly and gene regulation	en_US
dc.type	Journal Article
utslib.citation.volume	26	en_US
utslib.citation.volume	579	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
utslib.for	0903 Biomedical Engineering	en_US
utslib.for	0304 Medicinal and Biomolecular Chemistry	en_US
utslib.for	0603 Evolutionary Biology	en_US
dc.location.activity	ISI:000233002500005	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.issue	26	en_US
pubs.publication-status	Published	en_US
pubs.volume	579	en_US

Abstract:

RNAi is a conserved gene-specific regulatory mechanism, which silences target gene expression transcriptionally and post-transcriptionally. The RNAi machinery converts the sequence specific information of a long double stranded RNAs (dsRNAs) into small 21-22 nt long dsRNAs (siRNAs, miRNAs) which assemble into an effector complex, the RNA induced silencing complex (RISC). RISC assembly is asymmetric; one strand of an siRNA or a miRNA preferentially incorporates into the RNA-protein complex. Here, I review the rules of the asymmetric RISC formation and discuss their possible regulatory function in several steps in RNAi. © 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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http://hdl.handle.net/10453/15313