Blimp-1-dependent and -independent natural antibody production by B-1 and B-1-derived plasma cells.

Savage, HP; Yenson, VM; Sawhney, SS; Mousseau, BJ; Lund, FE; Baumgarth, N

Blimp-1-dependent and -independent natural antibody production by B-1 and B-1-derived plasma cells.

Savage, HP Yenson, VM Sawhney, SS Mousseau, BJ Lund, FE Baumgarth, N

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Publisher:: ROCKEFELLER UNIV PRESS
Publication Type:: Journal Article
Citation:: J Exp Med, 2017, 214, (9), pp. 2777-2794
Issue Date:: 2017-09-04

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Field	Value	Language
dc.contributor.author	Savage, HP
dc.contributor.author	Yenson, VM
dc.contributor.author	Sawhney, SS
dc.contributor.author	Mousseau, BJ
dc.contributor.author	Lund, FE
dc.contributor.author	Baumgarth, N
dc.date.accessioned	2022-01-28T00:43:28Z
dc.date.available	2017-06-08
dc.date.available	2022-01-28T00:43:28Z
dc.date.issued	2017-09-04
dc.identifier.citation	J Exp Med, 2017, 214, (9), pp. 2777-2794
dc.identifier.issn	0022-1007
dc.identifier.issn	1540-9538
dc.identifier.uri	http://hdl.handle.net/10453/153713
dc.description.abstract	Natural antibodies contribute to tissue homeostasis and protect against infections. They are secreted constitutively without external antigenic stimulation. The differentiation state and regulatory pathways that enable continuous natural antibody production by B-1 cells, the main cellular source in mice, remain incompletely understood. Here we demonstrate that natural IgM-secreting B-1 cells in the spleen and bone marrow are heterogeneous, consisting of (a) terminally differentiated B-1-derived plasma cells expressing the transcriptional regulator of differentiation, Blimp-1, (b) Blimp-1+, and (c) Blimp-1neg phenotypic B-1 cells. Blimp-1neg IgM-secreting B-1 cells are not simply intermediates of cellular differentiation. Instead, they secrete similar amounts of IgM in wild-type and Blimp-1-deficient (PRDM-1ΔEx1A) mice. Blimp-1neg B-1 cells are also a major source of IgG3. Consequently, deletion of Blimp-1 changes neither serum IgG3 levels nor the amount of IgG3 secreted per cell. Thus, the pool of natural antibody-secreting B-1 cells is heterogeneous and contains a distinct subset of cells that do not use Blimp-1 for initiation or maximal antibody secretion.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ROCKEFELLER UNIV PRESS
dc.relation.ispartof	J Exp Med
dc.relation.isbasedon	10.1084/jem.20161122
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	11 Medical and Health Sciences
dc.subject.classification	Immunology
dc.subject.mesh	Animals
dc.subject.mesh	Antibody Formation
dc.subject.mesh	B-Lymphocytes
dc.subject.mesh	Bone Marrow
dc.subject.mesh	Female
dc.subject.mesh	Immunoglobulin G
dc.subject.mesh	Male
dc.subject.mesh	Mice
dc.subject.mesh	Mice, Inbred C57BL
dc.subject.mesh	Plasma Cells
dc.subject.mesh	Positive Regulatory Domain I-Binding Factor 1
dc.subject.mesh	Spleen
dc.subject.mesh	Transcription Factors
dc.subject.mesh	Spleen
dc.subject.mesh	B-Lymphocytes
dc.subject.mesh	Plasma Cells
dc.subject.mesh	Bone Marrow
dc.subject.mesh	Animals
dc.subject.mesh	Mice, Inbred C57BL
dc.subject.mesh	Mice
dc.subject.mesh	Immunoglobulin G
dc.subject.mesh	Transcription Factors
dc.subject.mesh	Antibody Formation
dc.subject.mesh	Female
dc.subject.mesh	Male
dc.subject.mesh	Positive Regulatory Domain I-Binding Factor 1
dc.title	Blimp-1-dependent and -independent natural antibody production by B-1 and B-1-derived plasma cells.
dc.type	Journal Article
utslib.citation.volume	214
utslib.location.activity	United States
utslib.for	11 Medical and Health Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Health
pubs.organisational-group	/University of Technology Sydney/Faculty of Health/IMPACCT
utslib.copyright.status	open_access	*
dc.date.updated	2022-01-28T00:43:26Z
pubs.issue	9
pubs.publication-status	Published
pubs.volume	214
utslib.citation.issue	9

Abstract:

Natural antibodies contribute to tissue homeostasis and protect against infections. They are secreted constitutively without external antigenic stimulation. The differentiation state and regulatory pathways that enable continuous natural antibody production by B-1 cells, the main cellular source in mice, remain incompletely understood. Here we demonstrate that natural IgM-secreting B-1 cells in the spleen and bone marrow are heterogeneous, consisting of (a) terminally differentiated B-1-derived plasma cells expressing the transcriptional regulator of differentiation, Blimp-1, (b) Blimp-1+, and (c) Blimp-1neg phenotypic B-1 cells. Blimp-1neg IgM-secreting B-1 cells are not simply intermediates of cellular differentiation. Instead, they secrete similar amounts of IgM in wild-type and Blimp-1-deficient (PRDM-1ΔEx1A) mice. Blimp-1neg B-1 cells are also a major source of IgG3. Consequently, deletion of Blimp-1 changes neither serum IgG3 levels nor the amount of IgG3 secreted per cell. Thus, the pool of natural antibody-secreting B-1 cells is heterogeneous and contains a distinct subset of cells that do not use Blimp-1 for initiation or maximal antibody secretion.

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http://hdl.handle.net/10453/153713