Effects of spatial confinement on migratory properties of Dictyostelium discoideum cells.

Belotti, Y; McGloin, D; Weijer, CJ

Effects of spatial confinement on migratory properties of Dictyostelium discoideum cells.

Belotti, Y McGloin, D

Weijer, CJ

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Publisher:: Taylor & Francis Open Access
Publication Type:: Journal Article
Citation:: Communicative and Integrative Biology, 2021, 14, (1), pp. 5-14
Issue Date:: 2021-01-28

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Field	Value	Language
dc.contributor.author	Belotti, Y
dc.contributor.author	McGloin, D https://orcid.org/0000-0002-0075-4481
dc.contributor.author	Weijer, CJ
dc.date.accessioned	2022-03-18T05:18:41Z
dc.date.available	2022-03-18T05:18:41Z
dc.date.issued	2021-01-28
dc.identifier.citation	Communicative and Integrative Biology, 2021, 14, (1), pp. 5-14
dc.identifier.issn	1942-0889
dc.identifier.issn	1942-0889
dc.identifier.uri	http://hdl.handle.net/10453/155349
dc.description.abstract	Migratory environments of various eukaryotic cells, such as amoeba, leukocytes and cancer cells, typically involve spatial confinement. Numerous studies have recently emerged, aimed to develop experimental platforms that better recapitulate the characteristics of the cellular microenvironment. Using microfluidic technologies, we show that increasing confinement of Dictyostelium discoideum cells into narrower micro-channels resulted in a significant change in the mode of migration and associated arrangement of the actomyosin cytoskeleton. We observed that cells tended to migrate at constant speed, the magnitude of which was dependent on the size of the channels, as was the locomotory strategy adopted by each cell. Two different migration modes were observed, pseudopod-based and bleb-based migration, with bleb based migration being more frequent with increasing confinement and leading to slower migration. Beside the migration mode, we found that the major determinants of cell speed are its protrusion rate, the amount of F-actin at its leading edge and the number of actin foci. Our results highlighted the impact of the microenvironments on cell behavior. Furthermore, we developed a novel quantitative movement analysis platform for mono-dimensional cell migration that allows for standardization and simplification of the experimental conditions and aids investigation of the complex and dynamic processes occurring at the single-cell level.
dc.format	Electronic
dc.language	eng
dc.publisher	Taylor & Francis Open Access
dc.relation.ispartof	Communicative and Integrative Biology
dc.relation.isbasedon	10.1080/19420889.2021.1872917
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0601 Biochemistry and Cell Biology, 0603 Evolutionary Biology
dc.subject.classification	Developmental Biology
dc.title	Effects of spatial confinement on migratory properties of Dictyostelium discoideum cells.
dc.type	Journal Article
utslib.citation.volume	14
utslib.location.activity	United States
utslib.for	0601 Biochemistry and Cell Biology
utslib.for	0603 Evolutionary Biology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
utslib.copyright.status	open_access	*
pubs.consider-herdc	false
dc.date.updated	2022-03-18T05:18:37Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	14
utslib.citation.issue	1

Abstract:

Migratory environments of various eukaryotic cells, such as amoeba, leukocytes and cancer cells, typically involve spatial confinement. Numerous studies have recently emerged, aimed to develop experimental platforms that better recapitulate the characteristics of the cellular microenvironment. Using microfluidic technologies, we show that increasing confinement of Dictyostelium discoideum cells into narrower micro-channels resulted in a significant change in the mode of migration and associated arrangement of the actomyosin cytoskeleton. We observed that cells tended to migrate at constant speed, the magnitude of which was dependent on the size of the channels, as was the locomotory strategy adopted by each cell. Two different migration modes were observed, pseudopod-based and bleb-based migration, with bleb based migration being more frequent with increasing confinement and leading to slower migration. Beside the migration mode, we found that the major determinants of cell speed are its protrusion rate, the amount of F-actin at its leading edge and the number of actin foci. Our results highlighted the impact of the microenvironments on cell behavior. Furthermore, we developed a novel quantitative movement analysis platform for mono-dimensional cell migration that allows for standardization and simplification of the experimental conditions and aids investigation of the complex and dynamic processes occurring at the single-cell level.

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http://hdl.handle.net/10453/155349