Achieving complete nitrogen removal by coupling nitritation-anammox and methane-dependent denitrification: A model-based study.

Chen, X; Guo, J; Xie, G-J; Yuan, Z; Ni, B-J

Achieving complete nitrogen removal by coupling nitritation-anammox and methane-dependent denitrification: A model-based study.

Chen, X Guo, J Xie, G-J Yuan, Z Ni, B-J

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Publisher:: WILEY-BLACKWELL
Publication Type:: Journal Article
Citation:: Biotechnol Bioeng, 2016, 113, (5), pp. 1035-1045
Issue Date:: 2016-05

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Field	Value	Language
dc.contributor.author	Chen, X
dc.contributor.author	Guo, J
dc.contributor.author	Xie, G-J
dc.contributor.author	Yuan, Z
dc.contributor.author	Ni, B-J
dc.date.accessioned	2022-07-20T01:39:41Z
dc.date.available	2015-10-21
dc.date.available	2022-07-20T01:39:41Z
dc.date.issued	2016-05
dc.identifier.citation	Biotechnol Bioeng, 2016, 113, (5), pp. 1035-1045
dc.identifier.issn	0006-3592
dc.identifier.issn	1097-0290
dc.identifier.uri	http://hdl.handle.net/10453/159058
dc.description.abstract	The discovery of denitrifying anaerobic methane oxidation (DAMO) processes enables the complete nitrogen removal from wastewater by utilizing the methane produced on site from anaerobic digesters. This model-based study investigated the mechanisms and operational window for efficient nitrogen removal by coupling nitritation-anaerobic ammonium oxidation (Anammox) and methane-dependent denitrification in membrane biofilm reactors (MBfRs). A mathematical model was applied to describe the microbial interactions among Anammox bacteria, DAMO archaea, and DAMO bacteria. The model sufficiently described the batch experimental data from an MBfR containing an Anammox-DAMO biofilm with different feeding nitrogen compositions, which confirmed the validity of the model. The effects of process parameters on the system performance and microbial community structure could therefore be reliably evaluated. The impacts of nitritation produced NO2(-)/NH4(+) ratio, methane supply, biofilm thickness and total nitrogen (TN) surface loading were comprehensively investigated with the model. Results showed that the optimum NO2(-)/NH4(+) ratio produced from nitritation for the Anammox-DAMO biofilm system was around 1.0 in order to achieve the maximum TN removal (over 99.0%), independent on TN surface loading. The corresponding optimal methane supply increased while the associated methane utilization efficiency decreased with the increase of TN surface loading. The cooperation between DAMO organisms and Anammox bacteria played the key role in the TN removal. Based on these results, the proof-of-concept feasibility of a single-stage MBfR coupling nitritation-Anammox-DAMO for complete nitrogen removal was also tested through integrating the model with ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) processes whilst controlling the dissolved oxygen (DO) concentration in the simulated system. The maximum TN removal was found to be achieved at the bulk DO concentration of around 0.17 g m(-3) under the simulation conditions, with the AOB, Anammox bacteria and DAMO organisms coexisting in the biofilm.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	WILEY-BLACKWELL
dc.relation	http://purl.org/au-research/grants/arc/DE130100451
dc.relation	http://purl.org/au-research/grants/arc/DP130103147
dc.relation.ispartof	Biotechnol Bioeng
dc.relation.isbasedon	10.1002/bit.25866
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Biotechnology
dc.subject.mesh	Ammonium Compounds
dc.subject.mesh	Anaerobiosis
dc.subject.mesh	Archaea
dc.subject.mesh	Bacterial Physiological Phenomena
dc.subject.mesh	Biofilms
dc.subject.mesh	Bioreactors
dc.subject.mesh	Computer Simulation
dc.subject.mesh	Denitrification
dc.subject.mesh	Methane
dc.subject.mesh	Models, Biological
dc.subject.mesh	Nitrogen
dc.subject.mesh	Oxidation-Reduction
dc.subject.mesh	Waste Disposal, Fluid
dc.subject.mesh	Waste Water
dc.subject.mesh	Water Pollutants, Chemical
dc.subject.mesh	Biofilms
dc.subject.mesh	Archaea
dc.subject.mesh	Nitrogen
dc.subject.mesh	Ammonium Compounds
dc.subject.mesh	Methane
dc.subject.mesh	Water Pollutants, Chemical
dc.subject.mesh	Bioreactors
dc.subject.mesh	Waste Disposal, Fluid
dc.subject.mesh	Anaerobiosis
dc.subject.mesh	Oxidation-Reduction
dc.subject.mesh	Models, Biological
dc.subject.mesh	Computer Simulation
dc.subject.mesh	Bacterial Physiological Phenomena
dc.subject.mesh	Denitrification
dc.subject.mesh	Waste Water
dc.title	Achieving complete nitrogen removal by coupling nitritation-anammox and methane-dependent denitrification: A model-based study.
dc.type	Journal Article
utslib.citation.volume	113
utslib.location.activity	United States
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	closed_access	*
dc.date.updated	2022-07-20T01:39:39Z
pubs.issue	5
pubs.publication-status	Published
pubs.volume	113
utslib.citation.issue	5

Abstract:

The discovery of denitrifying anaerobic methane oxidation (DAMO) processes enables the complete nitrogen removal from wastewater by utilizing the methane produced on site from anaerobic digesters. This model-based study investigated the mechanisms and operational window for efficient nitrogen removal by coupling nitritation-anaerobic ammonium oxidation (Anammox) and methane-dependent denitrification in membrane biofilm reactors (MBfRs). A mathematical model was applied to describe the microbial interactions among Anammox bacteria, DAMO archaea, and DAMO bacteria. The model sufficiently described the batch experimental data from an MBfR containing an Anammox-DAMO biofilm with different feeding nitrogen compositions, which confirmed the validity of the model. The effects of process parameters on the system performance and microbial community structure could therefore be reliably evaluated. The impacts of nitritation produced NO2(-)/NH4(+) ratio, methane supply, biofilm thickness and total nitrogen (TN) surface loading were comprehensively investigated with the model. Results showed that the optimum NO2(-)/NH4(+) ratio produced from nitritation for the Anammox-DAMO biofilm system was around 1.0 in order to achieve the maximum TN removal (over 99.0%), independent on TN surface loading. The corresponding optimal methane supply increased while the associated methane utilization efficiency decreased with the increase of TN surface loading. The cooperation between DAMO organisms and Anammox bacteria played the key role in the TN removal. Based on these results, the proof-of-concept feasibility of a single-stage MBfR coupling nitritation-Anammox-DAMO for complete nitrogen removal was also tested through integrating the model with ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) processes whilst controlling the dissolved oxygen (DO) concentration in the simulated system. The maximum TN removal was found to be achieved at the bulk DO concentration of around 0.17 g m(-3) under the simulation conditions, with the AOB, Anammox bacteria and DAMO organisms coexisting in the biofilm.

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http://hdl.handle.net/10453/159058