Multiplexing surface anchored functionalized iron carbide nanoparticle: A low molecular weight proteome responsive nano-tracer.

Hasan, M; Gulzar, H; Zafar, A; Ul Haq, A; Mustafa, G; Tariq, T; Khalid, A; Mahmmod, A; Shu, X; Mahmood, N

Multiplexing surface anchored functionalized iron carbide nanoparticle: A low molecular weight proteome responsive nano-tracer.

Hasan, M Gulzar, H Zafar, A Ul Haq, A Mustafa, G Tariq, T Khalid, A Mahmmod, A Shu, X Mahmood, N

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Publisher:: ELSEVIER
Publication Type:: Journal Article
Citation:: Colloids Surf B Biointerfaces, 2021, 203, pp. 111746
Issue Date:: 2021-07

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Field	Value	Language
dc.contributor.author	Hasan, M
dc.contributor.author	Gulzar, H
dc.contributor.author	Zafar, A
dc.contributor.author	Ul Haq, A
dc.contributor.author	Mustafa, G
dc.contributor.author	Tariq, T
dc.contributor.author	Khalid, A
dc.contributor.author	Mahmmod, A
dc.contributor.author	Shu, X
dc.contributor.author	Mahmood, N
dc.date.accessioned	2022-07-21T23:13:46Z
dc.date.available	2021-04-01
dc.date.available	2022-07-21T23:13:46Z
dc.date.issued	2021-07
dc.identifier.citation	Colloids Surf B Biointerfaces, 2021, 203, pp. 111746
dc.identifier.issn	0927-7765
dc.identifier.issn	1873-4367
dc.identifier.uri	http://hdl.handle.net/10453/159097
dc.description.abstract	Harvesting the low molecular weight (LMW) proteins from the cellular exudates is a big challenge for early disease detection. Here, we introduce a unique probe composed of surface-functionalized Fe2C NPs with different functional groups to harvest, identify and profile differentially expressed biomarker proteins. Three different functionalization of Fe2C NPs with Fe2C@NH2, Fe2C@COOH and Fe2C@PEG enabled to harvest 119 differentially expressed proteins from HeLa cell exudates. Among these proteins, 57 were LMW which 82.46 % were up-regulated and 17.54 % were down-regulated. The Fe2C@NH2 were able to separate 60S ribosomal proteins L7a, and L11, and leucine-rich repeat-containing protein 59. These proteins play a vital role in the maturation of large subunit ribosomal ribonucleic acid, mRNA splicing via spliceosome and cancer cell inhibitor, respectively. While, Fe2C@COOH identifies the 60S ribosomal protein types L7, 40S ribosomal protein S11, and 60S ribosomal protein L24. These proteins were important for large ribosomal subunit biogenesis, translational initiation, and assembly of large subunit precursor of pre-ribosome. Finally, the Fe2C@PEG extracted 40S ribosomal protein S2, splicing factor, arginine/serine-rich and 40S ribosomal protein S4, X isoform which were responsible for nonsense-mediated decay, oligodendrocyte differentiation and multicellular organism development. Thus, these results help us in defining oncogenic biomarkers for early disease detection.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ELSEVIER
dc.relation.ispartof	Colloids Surf B Biointerfaces
dc.relation.isbasedon	10.1016/j.colsurfb.2021.111746
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0306 Physical Chemistry (incl. Structural), 0903 Biomedical Engineering, 0904 Chemical Engineering
dc.subject.classification	Chemical Physics
dc.subject.mesh	Carbon Compounds, Inorganic
dc.subject.mesh	HeLa Cells
dc.subject.mesh	Humans
dc.subject.mesh	Iron Compounds
dc.subject.mesh	Molecular Weight
dc.subject.mesh	Nanoparticles
dc.subject.mesh	Proteome
dc.subject.mesh	Saccharomyces cerevisiae Proteins
dc.subject.mesh	Hela Cells
dc.subject.mesh	Humans
dc.subject.mesh	Carbon Compounds, Inorganic
dc.subject.mesh	Iron Compounds
dc.subject.mesh	Saccharomyces cerevisiae Proteins
dc.subject.mesh	Proteome
dc.subject.mesh	Molecular Weight
dc.subject.mesh	Nanoparticles
dc.title	Multiplexing surface anchored functionalized iron carbide nanoparticle: A low molecular weight proteome responsive nano-tracer.
dc.type	Journal Article
utslib.citation.volume	203
utslib.location.activity	Netherlands
utslib.for	0306 Physical Chemistry (incl. Structural)
utslib.for	0903 Biomedical Engineering
utslib.for	0904 Chemical Engineering
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	closed_access	*
dc.date.updated	2022-07-21T23:13:42Z
pubs.publication-status	Published
pubs.volume	203

Abstract:

Harvesting the low molecular weight (LMW) proteins from the cellular exudates is a big challenge for early disease detection. Here, we introduce a unique probe composed of surface-functionalized Fe2C NPs with different functional groups to harvest, identify and profile differentially expressed biomarker proteins. Three different functionalization of Fe2C NPs with Fe2C@NH2, Fe2C@COOH and Fe2C@PEG enabled to harvest 119 differentially expressed proteins from HeLa cell exudates. Among these proteins, 57 were LMW which 82.46 % were up-regulated and 17.54 % were down-regulated. The Fe2C@NH2 were able to separate 60S ribosomal proteins L7a, and L11, and leucine-rich repeat-containing protein 59. These proteins play a vital role in the maturation of large subunit ribosomal ribonucleic acid, mRNA splicing via spliceosome and cancer cell inhibitor, respectively. While, Fe2C@COOH identifies the 60S ribosomal protein types L7, 40S ribosomal protein S11, and 60S ribosomal protein L24. These proteins were important for large ribosomal subunit biogenesis, translational initiation, and assembly of large subunit precursor of pre-ribosome. Finally, the Fe2C@PEG extracted 40S ribosomal protein S2, splicing factor, arginine/serine-rich and 40S ribosomal protein S4, X isoform which were responsible for nonsense-mediated decay, oligodendrocyte differentiation and multicellular organism development. Thus, these results help us in defining oncogenic biomarkers for early disease detection.

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http://hdl.handle.net/10453/159097